A5102958211- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Gastric Cancer Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Metastasis and carcinoma case studies
- Virus-based gene therapy research
- Hematopoietic Stem Cell Transplantation
- Gastrointestinal Tumor Research and Treatment
- RNA Interference and Gene Delivery
- Lung Cancer Treatments and Mutations
- Reproductive System and Pregnancy
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Organ Transplantation Techniques and Outcomes
- Vascular Tumors and Angiosarcomas
- Colorectal Cancer Treatments and Studies
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Diagnosis and Treatment
Nagasaki University
2021-2025
Nagasaki University Hospital
2022-2025
Fukuoka Dental College
2019-2022
Kyoto first Red Cross hospital
2021
Kyushu University
2010-2020
Cleveland Clinic
2017-2018
Cleveland Clinic Lerner College of Medicine
2018
National Sagamihara Hospital
2016
National Hospital Organization Kyushu Cancer Center
2016
Kyushu University Hospital
2013-2015
ObjectivesThe programmed death ligand 1(PD-L1)/programmed cell protein 1 (PD-1) pathway is one of the most important checkpoint pathways for mediating tumor-induced immune suppression through T-cell exhaustion. Recently, targeted therapies using monoclonal antibodies against components this have been shown to reduce tumor burden in patients with non–small lung cancer (NSCLC). However, prognostic significance PD-L1 expression controversial and precise mechanisms gene activation yet be...
Programmed death‐ligand 1 ( PD ‐L1) plays a crucial role in the host immune system cancer progression. The gene promoter region of ‐L1 also contains binding site for ZEB 1, transcription factor related to epithelial‐mesenchymal transition EMT ). purpose this study was clarify relationship between and its clinical importance esophageal squamous cell carcinoma ESCC expression at tumor invasive front examined by immunohistochemistry resected specimens from 90 patients with who underwent surgery...
Autophagy is a homeostatic mechanism that regulates protein and organelle turnover uses the amino acids from degraded proteins to produce adenosine 5′-triphosphate (ATP). We investigated activity of autophagy-associated pathways in liver regeneration after partial hepatectomy (PHx) liver-specific autophagy-related gene 5 (Atg5) knockout (KO) mice. Liver was severely impaired by 70% PHx, with reduction postoperative mitosis, but compensating increase hepatocyte size. PHx induced intracellular...
Immunotherapy is reportedly effective in colorectal cancers (CRCs) with high microsatellite instability (MSI-H); however, the specific cell types that respond to immune checkpoint therapy are unclear. Herein, we aimed examine expression of programmed death-ligand 1 (PD-L1) and related proteins MSI-H microsatellite-stable (MSS) CRCs investigate microenvironment at tumor's invasive front. The MSI status was retrospectively assessed 499 patients undergoing surgical resection primary CRC; these,...
Rheumatoid arthritis (RA), a systemic inflammatory disease of unknown etiology, mainly affects synovial joints. Although angiogenic growth factors, including fibroblast factor-2 (FGF-2) and vascular endothelial factor (VEGF), may play critical role in the development progression RA joint disease, little information is now available regarding their exact initiation and/or RA. In this study, we show that both polypeptides were up-regulated rat tissue an adjuvant-induced model (AIA), as well...
Abstract It is widely accepted that angiogenesis required for tumor progression. Vascular endothelial growth factor (VEGF) a key molecule angiogenesis; however, its expressional regulation not well understood during all stages of tumorigenesis. Using cell lines and surgical specimens human non–small lung cancers (NSCLCs), we here show platelet-derived factor-AA (PDGF-AA) an essential autocrine regulator VEGF expression. To directly assess the expression PDGF-AA–dependent roles in...
Abstract Background Although radiotherapy is commonly used to treat head and neck cancer, it may lead radiation-associated dysphagia (RAD). There are various causes of RAD, however, the mechanism has not yet been fully identified. Currently, only effective treatment for RAD rehabilitation. Additionally, there few available animal models necessitating development new establish evaluate treatments. We hypothesize that radiation-induced muscle fibrosis could be one due impairment laryngeal...
Desmoid tumors are rare, locally invasive, non-metastasizing intermediate grade neoplasms. According to the organ procurement and transplantation network report, approximately one million solid transplants have been performed globally, from which only three desmoid reported. We present case of a 61-year-old male who developed an asymptomatic abdominal mass 3 years after kidney transplantation. Computed tomography revealed 50-mm attached Hem-o-lok clip beneath right rectus abdominis muscle. A...
Hepatocyte growth factor (HGF) is a potent angiogenic polypeptide that stimulates angiogenesis. Transcriptional regulation of HGF, however, has not been fully defined, with the exception hypoxia-mediated downregulation in cultured cells. In present study, we report factors, including were upregulated murine model critical limb ischemia vivo, finding was conflict previous vitro data. Mice deficient basic fibroblast factor-2 (FGF-2) showed reduced induction HGF protein ischemic muscles, and...
Abstract TRAIL and agonistic death receptor-specific antibodies can induce apoptosis in cancer cells with little cytotoxicity to normal cells. To improve TRAIL-induced antitumor effects, we tested its effectiveness combination pifithrin (PFT)-μ, which has the potential inhibit HSP70 function autophagy, both of participate resistance Among four human pancreatic cell lines tested, MiaPaca-2, Panc-1, BxPC-3 showed varying sensitivities TRAIL. In MiaPaca-2 Panc-1 cells, knockdown or beclin-1,...
Programmed death 1 (PD-1) is an immunoinhibitory receptor and has been identified as a new target for immunotherapy in cancer. Here we report the expression of PD-1 ligand (PD-L1) surgically resected gastric cancer.We examined formalin-fixed tumor samples from 144 cancer patients with primary diagnosis carcinoma. Immunohistochemistry was used to detect PD-L1. Human epidermal growth factor 2 (HER2) phosphatase tensin homolog (PTEN) loss heterozygosity were investigated these patients. RNA...
Abstract Immunosuppressive therapy for organ transplantation is essential controlling rejection. When liver performed as a hepatocellular carcinoma (HCC), recurrent HCC one of the most fatal complications. In this study, we show that intratumoral murine IL-12 (mIL-12) gene has potential to be an effective treatment malignancies under immunosuppression. C3H mice (H-2k), injected with FK506 (3 mg/kg) i.p., were s.c. implanted 2.5 × 106 MH134 cells (H-2k) and treated established...
Diabetic foot is caused by microangiopathy and suggested to be a result of impaired angiogenesis. Using severe hindlimb ischemia model streptozotocin-induced diabetic mice (STZ-DM), we show that disease solely the disturbance platelet-derived growth factor B-chain homodimer (PDGF-BB) expression but not responses angiogenic factors. STZ-DM frequently lost their hindlimbs after induced ischemia, whereas non-DM did not. Screening angiogenesis-related factors revealed only PDGF-BB was in on...
Discovery of the common and ubiquitous molecular targets for disruption angiogenesis, that are independent characteristics malignant tumors, is desired to develop more effective antitumor drugs. In this study, we propose platelet-derived growth factor receptor-α (PDGFRα)-p70S6K signal transduction pathway in mesenchymal cells, which required functional angiogenesis induced by fibroblast factor-2, potent candidate. Using murine limb ischemia as a tumor-free assay system, demonstrated p70S6K...
Abstract Dendritic cell (DC)-based cancer immunotherapy has been paid much attention as a new and cell-specific therapeutic in the last decade; however, little clinical outcome reported. Current limitations of DC-based include sparse information about which DC phenotype should be administered. We here report unique, representative, powerful method to activate DCs, namely recombinant Sendai virus-modified DCs (SeV/DC), for immunotherapy. In vitro treatment SeV without any bioactive gene...