A5017716828- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Research and Treatments
- Hematopoietic Stem Cell Transplantation
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Erythrocyte Function and Pathophysiology
- Pluripotent Stem Cells Research
- Acute Myeloid Leukemia Research
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Immunotherapy and Biomarkers
- Respiratory viral infections research
- Blood groups and transfusion
- Cytomegalovirus and herpesvirus research
- Chronic Myeloid Leukemia Treatments
- Blood disorders and treatments
- Neonatal Health and Biochemistry
- Immune Response and Inflammation
- Chronic Lymphocytic Leukemia Research
- Renal and related cancers
The University of Tokyo
2015-2024
Kyushu University Hospital
2010-2021
University of Tokyo Hospital
2020
Kyushu University
2010-2019
Fukuoka University
2019
Pharmaceuticals and Medical Devices Agency
2018
Tokyo University of Science
1993-2017
St. Petersburg Institute of Bioregulation and Gerontology
2017
In-Q-Tel
2014
ID Pharma (Japan)
2014
Transfusion of red blood cells (RBCs) is a standard and indispensable therapy in current clinical practice. In vitro production RBCs offers potential means to overcome shortage transfusable some situations also provide source free from possible infection or contamination by microorganisms. Thus, may become procedure the future. We previously reported successful establishment immortalized mouse erythroid progenitor cell lines that were able produce mature very efficiently. Here, we have...
Abstract Although oncolytic virotherapy is a promising anticancer therapy, antitumor efficacy hampered by low tumor selectivity. To identify potent and selective virotherapy, we carried out large-scale two-step screening of 28 enteroviral strains found that coxsackievirus B3 (CVB3) possessed specific activity against nine human non–small cell lung cancer (NSCLC) lines. CVB3-mediated cytotoxicity was positively correlated with the expression viral receptors, adenovirus receptor,...
Partial cDNA clones encoding human cytosolic aldehyde dehydrogenase (ALDH1) and mitochondrial (ALDH2) were isolated from a liver library constructed in phage lambda gt11. The expression was screened by using rabbit antibodies against ALDH1 ALDH2. Positive thus obtained subsequently with mixed synthetic oligonucleotides compatible peptide sequences of One the positive for contained an insertion 1.6 kilobase pairs (kbp). insert encoded 340 amino acid residues had 3' noncoding region 538 bp...
The Pebpb2 gene encodes a non-DNA binding subunit of the heterodimeric transcription factor, polyomavirus enhancer protein 2/core factor (PEBP2/CBF), and is rearranged in inversion chromosome 16 associated with human acute myeloid leukemia. To investigate its physiological function, was mutated by targeting strategy to generate null mutant. homozygous mutation mice proved lethal embryos around embryonic day 12.5, apparently due massive hemorrhaging central nervous system. In addition,...
The successful establishment of human embryonic stem cell (hESC) lines has inaugurated a new era in regenerative medicine by facilitating the transplantation differentiated ESCs to specific organs. However, problems with safety and efficacy hESC therapy vivo remain be resolved. Preclinical studies using animal model systems, including nonhuman primates, are essential evaluate therapies. Previously, we demonstrated that common marmosets suitable laboratory models for preclinical hematopoietic...
tob is a member of antiproliferative family genes. Mice lacking are prone to spontaneous formation tumors. The occurrence rate diethylnitrosamine-induced liver tumors higher in −/− mice than wild-type mice. p53 show accelerated tumor comparison with single null Expression cyclin D1 mRNA increased the absence Tob and reduced by Tob. acts as transcriptional corepressor suppresses promoter activity through an interaction histone deacetylase. Levels often decreased human cancers, implicating...
ABSTRACT In order to determine the parameters that govern activity of a ribozyme in vivo, we made systematic analysis chimeric tRNA Val ribozymes by measuring their cleavage activities vitro as well steady-state levels transcripts, half-lives transcribed ribozymes, and both HeLa H9 cells. These analyses were conducted use transient expression systems cells stable transformants express ribozymes. Localization transcripts appeared be determined higher-order structure each ribozyme. Since...
We produced lethally irradiated retrovirally GM-CSF-transduced autologous renal tumor cell vaccines (GVAX) from six Japanese patients with stage IV cancer (RCC). Four received GVAX ranging 1.4 × 108 to 3.7 cells on 6–17 occasions. Throughout a total of 48 vaccinations, there were no severe adverse events. After vaccination, DTH skin tests became positive RCC (auto-RCC) in all patients. The vaccination sites showed significant infiltration by CD4+ T cells, eosinophils, and HLA-DR-positive...
Abstract We successfully established a human gastric cancer cell line, HuGC‐OOHIRA, from the ascites of 60‐year‐old patient with advanced (poorly differentiated adenocarcinoma) complicated by peritonitis carcinomatosa and leukocytosis unknown origin. Morphologically, cells were polygonal adhered weakly to culture flask. They tended pile up upon reaching confluence. Chromosome analysis revealed that line has two modes chromosome number, namely near diploidy tetraploidy. Double minutes (DMs)...
A CD30 ligand (CD30L; CD153) and its receptor, CD30, is a membrane-associated glycoprotein belonging to the TNF superfamily TNFR superfamily. These were expressed preferentially by activated CD4(+)T cells. In this paper, we show that CD44(low)CD62(hi)CD4(+) T cells from CD30L(-/-) or CD30(-/-) mice exhibited impaired differentiation into Th17 but an increased ability produce IL-2 after in vitro culture under Th17-polarizing conditions. Neutralization with anti-IL-2 mAb partly restored of...
Although various therapies are available to treat cancers, including surgery, chemotherapy, and radiotherapy, cancer has been the leading cause of death in Japan for last 30 years, new therapeutic modalities urgently needed. As a modality, there recently great interest oncolytic virotherapy, with measles virus being candidate expected show strong antitumor effects. The efficacy however, was strongly limited by host immune response previous clinical trials. To enhance prolong activity we...
Abstract Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Innovative treatment required to improve overall survival rates for advanced NSCLC. Oncolytic virotherapy using enteroviruses has emerged as a promising anticancer strategy. To identify novel, potent with an improved safety profile, we assessed oncolytic activity 28 enteroviral strains and focused on coxsackievirus A11 (CVA11). CVA11 infection caused extensive in all three examined human...
Summary. Cytomegalovirus (CMV) infection in 28 adult patients after cord blood transplantation (CBT) from unrelated donors was compared with that bone marrow HLA (human leucocyte antigen)‐matched related (R‐BMT) and (U‐BMT) donors. Positive CMV antigenaemia seen 19 (79%) of 24 CMV‐seropositive at a median 42 d (range 29–85 d) CBT, but zero four CMV‐seronegative patients. This did not differ significantly values observed R‐BMT U‐BMT (66%, P = 0·22, 60%, 0·15 respectively). Based on the...