A5105407717- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Angiogenesis and VEGF in Cancer
- Platelet Disorders and Treatments
- Cancer Research and Treatments
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Drug Transport and Resistance Mechanisms
- Cancer therapeutics and mechanisms
- Adipose Tissue and Metabolism
- Lung Cancer Treatments and Mutations
- HER2/EGFR in Cancer Research
- Gene expression and cancer classification
- PI3K/AKT/mTOR signaling in cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Carcinogens and Genotoxicity Assessment
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
Central Institute for Experimental Animals
2011-2025
Nippon Chemiphar (Japan)
2023
BioScience Laboratories (United States)
2014-2015
Sekisui Medical (Japan)
2014
Nara Institute of Science and Technology
2007
University of Tsukuba
2003-2006
Nissan Chemical Corporation (Japan)
2006
Tokyo Metropolitan Institute of Medical Science
2006
Keio University
2006
Tokai University
1993-2005
The adipocyte-derived hormone adiponectin has been shown to play important roles in the regulation of energy homeostasis and insulin sensitivity. In this study, we analyzed globular domain (gAd) transgenic (Tg) mice crossed with leptin-deficient ob/ob or apoE-deficient mice. Interestingly, despite an unexpected similar body weight, gAd Tg showed amelioration resistance β-cell degranulation as well diabetes, indicating that leptin appeared have both distinct overlapping functions....
Adipose tissue expression and circulating concentrations of monocyte chemoattractant protein-1 (MCP-1) correlate positively with adiposity. To ascertain the roles MCP-1 overexpression in adipose, we generated transgenic mice by utilizing adipocyte P2 (aP2) promoter (aP2-MCP-1 mice). These had higher plasma increased macrophage accumulation adipose tissues, as confirmed immunochemical, flow cytometric, gene analyses. Tumor necrosis factor-α interleukin-6 mRNA levels white non-esterified fatty...
Abstract Unlike protein-coding genes, the majority of human long non-coding RNAs (lncRNAs) are considered non-conserved. Although lncRNAs have been shown to function in diverse pathophysiological processes mice, it remains largely unknown whether such vivo functions. Here, we describe an integrated pipeline define non-conserved lncRNAs. We first identify with high potential using multiple indicators derived from genetic data related cardiometabolic traits, then lncRNA’s and specific target...
The successful establishment of human embryonic stem cell (hESC) lines has inaugurated a new era in regenerative medicine by facilitating the transplantation differentiated ESCs to specific organs. However, problems with safety and efficacy hESC therapy vivo remain be resolved. Preclinical studies using animal model systems, including nonhuman primates, are essential evaluate therapies. Previously, we demonstrated that common marmosets suitable laboratory models for preclinical hematopoietic...
Hundreds of inbred mouse strains are established for use in a broad spectrum basic research fields, including genetics, neuroscience, immunology, and cancer. Inbred mice exhibit identical intra-strain genetics divergent inter-strain phenotypes. The cognitive behavioral divergences must be controlled by the variances structure function their brains; however, underlying morphological features strain-to-strain difference remain obscure. Here, vivo microscopic magnetic resonance imaging was...
Background: Amplification and rearrangement of the epidermal growth factor receptor (EGFR) gene is frequently associated with malignant gliomas. One type EGFR mutation in primary gliomas results overexpression an aberrant messenger RNA (mRNA) that lacks sequences exons II through VI human gene. We observed aberrantly spliced mRNA contains a ribozyme cleavable sequence (5*-AAG GUA AUU-3*) created by joining exon I to VII. hypothesized appropriately designed could mediate site-specific...
We developed a reliable new model system for assaying liver metastasis using NOD/SCID/gamma(c)(null) (NOG) mice. Seven human pancreatic cancer cell lines were examined their ability to form diverse metastatic foci in the livers of NOD/SCID and NOG Capan-2 PL45 showed no when seeded at up 10(5) cells both strains, BxPC-3 was observed The mouse detected only AsPC-1 line inoculated with >10(3) cells. In contrast, 10(2) MIA PaCa-2, PANC-1 cells, evident 71.4% (5/7), 57.1% (4/7) 37.5% (3/8) mice,...
Human tumor tissue line models established in the severely immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Sug/Jic (NOD/Shi-scid, IL-2Rγnull or NOG) mouse are important tools for oncology research. During establishment process, a lymphoproliferative lesion (LPL) that replaces original cells site of transplantation occurs. In present study, we studied impact LPL on process and characteristics lesion, investigated systemic distribution mouse, evaluated potential simple identification method. The...
Eight lines of human breast tumors implanted in nude mice were treated with various antitumor agents at two different doses, maximum tolerated doses (MTD) and rational (RD) that pharmacokinetically equivalent to the clinical doses; response rates both compared. With MTD, mitomycin C vinblastine 100%, those other including cyclophosphamide, nimustine (a water-soluble nitrosourea), vincristine, Adriamycin (doxorubicin; Adria Laboratories, Columbus, OH), 5-fluorouracil (5-FU), methotrexate...
Abstract Background Epidemiological studies have suggested that the encounter with commensal microorganisms during neonatal period is essential for normal development of host immune system. Basic research involving gnotobiotic mice has demonstrated colonization at age 5 weeks too late to reconstitute function. In this study, we examined transcriptome profiles large intestine (LI), small (SI), liver (LIV), and spleen (SPL) 3 bacterial models—specific pathogen - free (SPF), ex-germ-free...
To examine the drug efficacy of a novel farnesyltransferase inhibitor (FTI), CH4512600, in vivo, we developed reliable liver metastasis model human colon cancer using NOD/Shi-scid IL2Rγnull (NOG) mice. Eleven cell lines were examined for their ability to form diverse metastatic foci livers NOG When inoculated with 104 COLO320DM, HCT 116, HT-29, WiDr, LoVo and LS174T cells, was evident 100% (6/6), 88.9% (8/9), 87.5% (7/8), 83.3% (5/6) 50.0% (3/6) mice, respectively. CaCo2, COLO201, LS123,...
The purpose of tumorigenicity testing, as applied not only to cell substrates used for viral vaccine manufacture but also stem cells cell-based therapy, is discriminate between that have the capacity form tumors and do not. Therefore, testing essential in assessing safety these biological materials. Recently developed NOD/Shi-scid IL2Rgnull (NOG) mice been shown be superior (SCID) xenotransplantation both normal cancerous cells. To select a suitable mouse strain xenogenic host we compared...
3'-Hydroxy-4'-methoxydiclofenac (VI) is a human-specific metabolite known to accumulate in the plasma of patients after repeated administration diclofenac sodium. Diclofenac also produces glutathione-conjugated metabolites, some which are human-specific. In present study, we investigated whether these metabolites could be generated humanized chimeric mice produced from TK-NOG mice. After single oral mice, unchanged drug peaked at 0.25 hour and then declined with half-life (t1/2) 2.4 hours....
Thrombocytopenia is a common problem in the management of patients with cancer and other conditions that affect hematopoietic cells. In previous clinical trials, polyethylene-glycol-conjugated recombinant human megakaryocyte growth development factor increased platelet counts idiopathic thrombocytopenic purpura solid tumors undergoing chemotherapy. However, antibodies to develop healthy volunteers chemotherapy cross-react endogenous thrombopoietin. As result, was discontinued 1998. The aim...
The NOD/Shi-scid, IL-2Rgamma(null) (NOG) mouse is a severely immunodeficient used for the engraftment of human tissues and cells. In this study, 2406 mice (8-62 weeks old, 503 males 1903 females) were subcutaneously engrafted with tissues. 16 (12-26 1 male 15 females), mass was seen in anteroventralis thorax on gross examination an incidence 0.7%. Histologically, masses composed sheets lymphoblastic A 'starry sky' pattern observed numerous mitoses. Immunohistochemically cells positive Thy 1....
Citation Velayuthaprabhu S, Matsubayashi H, Sugi T, Nakamura M, Ohnishi Y, Ogura Tomiyama Archunan G. A unique preliminary study on placental apoptosis in mice with passive immunization of anti-phosphatidylethanolamine antibodies and anti-factor XII antibodies. Am J Reprod Immunol 2011; 66: 373–384 Problem Antiphospholipid have been investigated both humans animal models. In contrast, there are fewer reports describing (aPE) humans, no studies aPE till date. Clinically, FXII deficiency or...