A5078146127- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Lung Cancer Treatments and Mutations
- Genomics and Phylogenetic Studies
- Mechanisms of cancer metastasis
- Genetic factors in colorectal cancer
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Immune Cell Function and Interaction
- Traditional Chinese Medicine Studies
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Cancer-related gene regulation
- Genomics and Rare Diseases
- COVID-19 epidemiological studies
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Lung Cancer Research Studies
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
Nagoya University
2002-2025
Aichi Cancer Center
2019-2025
The University of Tokyo
2015-2024
Tokyo University of Science
2012-2024
Kumamoto Health Science University
2016-2021
Kyoto University
2014-2021
Kumamoto University
2016-2017
Kagoshima University
2009-2015
Tokyo Metropolitan Institute of Medical Science
2015
Keio University
2014
Abstract Activation of the EGFR, KRAS, and ALK oncogenes defines 3 different pathways molecular pathogenesis in lung adenocarcinoma. However, many tumors lack activation any pathway (triple-negative adenocarcinomas) posing a challenge for prognosis treatment. Here, we report an extensive genome-wide expression profiling 226 primary human stage I–II adenocarcinomas that elucidates characteristics harbor mutations or mutations, is, triple-negative adenocarcinomas. One hundred seventy-four...
Triple negative breast cancer (TNBC) has a poor outcome due to the lack of beneficial therapeutic targets. To clarify molecular mechanisms involved in carcinogenesis TNBC and identify target molecules for novel anticancer drugs, we analyzed gene expression profiles 30 TNBCs as well 13 normal epithelial ductal cells that were purified by laser-microbeam microdissection. We identified 301 321 transcripts significantly upregulated downregulated TNBC, respectively. In particular, profile...
Removal of senescent cells (senolysis) has been proposed to be beneficial for improving age-associated pathologies, but the molecular pathways such senolytic activity have not yet emerged. Here, we identified glutaminase 1 (GLS1) as an essential gene survival human cells. The intracellular pH in was lowered by lysosomal membrane damage, and this induced kidney-type (KGA) expression. resulting enhanced glutaminolysis ammonia production, which neutralized lower improved Inhibition...
Abstract Purpose: KRAS is among the most commonly mutated oncogene in cancer including non–small cell lung (NSCLC). In early clinical trials, inhibitors targeting G12C-mutant have achieved responses some patients with NSCLC. Possible intrinsic and acquired resistance mechanisms to G12C are not fully elucidated will likely become important identify. Experimental Design: To identify potential mechanisms, we defined sensitivity of a panel G12C–mutant lines inhibitor, AMG510. Gene set enrichment...
Abstract Background To understand the molecular mechanisms underlying important biological processes, a detailed description of gene products networks involved is required. In order to define and such networks, some statistical methods are proposed in literature estimate regulatory from time-series microarray data. However, several problems still need be overcome. Firstly, information flow inferred, addition correlation between genes. Secondly, we usually try identify large number genes...
Purpose To identify stage I lung adenocarcinoma patients with a poor prognosis who will benefit from adjuvant therapy. Patients and Methods Whole gene expression profiles were obtained at 19 time points over 48-hour course human primary epithelial cells that stimulated epidermal growth factor (EGF) in the presence or absence of clinically used EGF receptor tyrosine kinase (RTK)-specific inhibitor, gefitinib. The data subjected to mathematical simulation using State Space Model (SSM)....
A primary tumor can create a premetastatic niche in distant organs to facilitate the development of metastasis. The mechanism by which cells communicate with host develop niches is unclear. We focused on role microRNA (miR) signaling promoting Here, we identified miR-203 as molecule between tumors and monocytes metastatic colorectal cancer (CRC) patients. Notably, high expression serum exosomal miR-203, major form circulation, was associated metastasis an independent poor prognostic factor,...
Allogeneic hematopoietic stem cell transplantation (HSCT) is one of curative treatment options for patients with hematologic malignancies. Although GVHD mediated by the donor’s T lymphocytes remains most challenging toxicity allo-HSCT, graft-versus-leukemia (GVL) effect targeting leukemic cells, has an important role in affecting overall outcome AML. Here we comprehensively characterized TCR repertoire who underwent matched donor or haplo-cord HSCT using next-generation sequencing approach....
Immune checkpoint inhibitors and adoptive transfer of gene-engineered T cells have emerged as novel therapeutic modalities for hard-to-treat solid tumors; however, many patients are refractory to these immunotherapies, the mechanisms underlying tumor immune resistance not been fully elucidated. By comparing microenvironment inhibition–sensitive –resistant murine tumors, we observed that resistant tumors had low immunogenicity. We identified antigen presentation by CD11b+F4/80+...
Abstract Background There are several types of pancreatic mass, so it is important to distinguish between them before treatment. Artificial intelligence (AI) a mathematical technique that automates learning and recognition data patterns. This study aimed investigate the efficacy our AI model using endoscopic ultrasonography (EUS) images multiple mass (pancreatic ductal adenocarcinoma [PDAC], adenosquamous carcinoma [PASC], acinar cell [ACC], metastatic tumor [MPT], neuroendocrine [NEC],...
Small cell lung cancer (SCLC) is the most aggressive type of cancer. Only 15% SCLC patients survive beyond 2 years after diagnosis. Therefore, for improvement patients’ outcome in this disease, it necessary to identify genetic alterations applicable as therapeutic targets cells. The purpose study identification genes frequently mutated and expressed SCLCs that will be targetable therapy patients. Exome sequencing was performed 28 primary tumors 16 metastatic from 38 with SCLCs. Expression...
The acquisition of endocrine resistance is a common obstacle in therapy patients with oestrogen receptor-α (ERα)-positive breast tumours. We previously demonstrated that the BIG3–PHB2 complex has crucial role modulation oestrogen/ERα signalling cancer cells. Here we report cell-permeable peptide inhibitor, called ERAP, regulates multiple ERα-signalling pathways associated tamoxifen cells by inhibiting interaction between BIG3 and PHB2. Intrinsic PHB2 released from ERAP directly binds to both...
Immune responses play a critical role in various disease conditions including cancer and autoimmune diseases. However, to date, there has not been rapid, sensitive, comprehensive, quantitative analysis method examine T-cell or B-cell immune responses. Here, we report new approach characterize T cell receptor (TCR) repertoire by sequencing millions of cDNA TCR α β chains combination with newly-developed algorithm. Using samples from lung patients treated peptide vaccines as model, demonstrate...
Abstract The genomic characteristics of dedifferentiated liposarcoma (DDLPS) that are associated with clinical features remain to be identified. Here, we conduct integrated whole exome and RNA sequencing analysis in 115 DDLPS tumors perform comparative well-differentiated components from eight samples. Several somatic copy-number alterations (SCNAs), including the gain 12q15, identified as frequent alterations. CTDSP1/2-DNM3OS fusion genes a subset tumors. Based on association SCNAs...
Although recent cancer genomics studies have identified a large number of genes that were mutated in human cancers, p53 remains as the most frequently gene. To further elucidate p53-signalling network, we performed transcriptome analysis on 24 tissues p53+/+ or p53-/- mice after whole-body X-ray irradiation. Here found transactivation total 3551 one more only mice, while 2576 downregulated. mRNA expression level each tissue was significantly associated with upregulated by Annotation using...
This study identifies signaling pathways essential for maintaining the stemness and metastatic potential of colorectal cancer cells proposes CREB as a therapeutic target in cancer.
Background A better understanding of the tumor immune microenvironment (TIME) will facilitate development prognostic biomarkers and more effective therapeutic strategies in patients with lung cancer. However, little has been reported on comprehensive evaluation complex interactions among cancer cells, local immunosuppressive elements TIME. Methods Whole-exome sequencing RNA were carried out 113 cancers. We performed single sample gene set enrichment analysis TIME-related sets to develop a...
Abstract Current KRAS G12C (OFF) inhibitors that target inactive GDP-bound cause responses in less than half of patients and these are not durable. A class RAS (ON) targets active GTP-bound blocks ERK signaling more potently the inactive-state inhibitors. Sensitivity to either agents is strongly correlated with inhibition mTORC1 activity. We have previously shown PI3K/mTOR ERK-signaling pathways converge on key cellular processes both required for significant antitumor find here combination...
Abstract Motivation: Statistical inference of gene networks by using time-course microarray expression profiles is an essential step towards understanding the temporal structure regulatory mechanisms. Unfortunately, most current studies have been limited to analysing a small number genes because length fairly short. One promising approach overcome such limitation infer exploring potential transcriptional modules which are sets sharing common function or involved in same pathway. Results: In...