A5012262880- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Histone Deacetylase Inhibitors Research
- Bladder and Urothelial Cancer Treatments
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Tissue Engineering and Regenerative Medicine
- S100 Proteins and Annexins
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
- Chromatin Remodeling and Cancer
- Immune Cell Function and Interaction
- Ferroptosis and cancer prognosis
- Pancreatic and Hepatic Oncology Research
- Phagocytosis and Immune Regulation
- Genomics and Chromatin Dynamics
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
National University Cancer Institute, Singapore
2016-2025
National University of Singapore
2016-2025
Guangzhou Medical University
2025
Guangzhou Institutes of Biomedicine and Health
2025
University of Hong Kong
2025
National University Health System
2016-2024
Second Affiliated Hospital of Xi'an Jiaotong University
2023
Bioinformatics Institute
2017
University of South Australia
2016
South Australia Pathology
2016
The phenotypic transformation of well-differentiated epithelial carcinoma into a mesenchymal-like state provides cancer cells with the ability to disseminate locally and metastasise. Different degrees epithelial–mesenchymal transition (EMT) have been found occur in carcinomas from breast, colon ovarian (OC), among others. Numerous studies focused on bona fide mesenchymal states but rarely intermediate states. In this study, we describe model system for appraising spectrum EMT using 43...
Epithelial-to-mesenchymal transition (EMT) mediates cancer cell invasion, metastasis, and drug resistance, but its impact on immune surveillance has not been explored. In this study, we investigated the functional consequences of mode epithelial plasticity targeted lysis by cytotoxic T lymphocytes (CTL). Acquisition EMT phenotype in various derivatives MCF-7 human breast cells was associated with dramatic morphologic changes actin cytoskeleton remodeling, CD24(-)/CD44(+)/ALDH(+) stem...
PD-L1 expression and regulation by mesenchymal tumor cells remain largely undefined. Here, we report that among different EMT-activated MCF7 human breast cancer cell clones, was differentially upregulated in sh-WISP2, MCF7–1001/2101, MDA-MB-231 but not SNAI1 SNAI1–6SA cells. Mechanistic investigations revealed siRNA silencing of ZEB-1, SNAI1, TWIST, or SLUG overexpression miR200 family members sh-WISP2 strongly decreased expression. Thus, propose depends on the EMT-TF involved EMT...
The enumeration of EpCAM-positive circulating tumor cells (CTCs) has allowed estimation overall metastatic burden in breast cancer patients. However, a thorough understanding CTCs associated with brain metastasis (BCBM) is necessary for early identification and evaluation treatment response to BCBM. Here we report that BCBM enriched distinct sub-population identifiable by their biomarker expression mutational content. Deriving from comprehensive analysis CTC transcriptomes, discovered unique...
Abstract Purpose: KRAS is among the most commonly mutated oncogene in cancer including non–small cell lung (NSCLC). In early clinical trials, inhibitors targeting G12C-mutant have achieved responses some patients with NSCLC. Possible intrinsic and acquired resistance mechanisms to G12C are not fully elucidated will likely become important identify. Experimental Design: To identify potential mechanisms, we defined sensitivity of a panel G12C–mutant lines inhibitor, AMG510. Gene set enrichment...
Knudson's "two-hit" paradigm posits that carcinogenesis requires inactivation of both copies an autosomal tumor suppressor gene. Here, we report the glycolytic metabolite methylglyoxal (MGO) transiently bypasses by inactivating breast cancer protein BRCA2 to elicit a cancer-associated, mutational single-base substitution (SBS) signature in nonmalignant mammary cells or patient-derived organoids. Germline monoallelic mutations predispose these changes. An analogous SBS signature, again...
// Bee Luan Khoo 1, * , Soo Chin Lee 2, 3, Prashant Kumar 4,* Tuan Zea Tan 3 Majid Ebrahimi Warkiani 5, 6 Samuel GW. Ow 2 Sayantani Nandi 4 Chwee Teck Lim 4, 7, 8, Jean Paul Thiery 9, 1 Mechanobiology Institute, National University of Singapore, Singapore Department Hematology-Oncology, Cancer Hospital, Science Institute Molecular and Cell Biology, A*STAR (Agency for Science, Technology Research), 5 BioSystems Micromechanics (BioSyM) IRG, Singapore-MIT Alliance Research (SMART) Centre,...
Abstract Epithelial-mesenchymal transition (EMT), a biological process by which polarized epithelial cells convert into mesenchymal phenotype, has been implicated to contribute the molecular heterogeneity of ovarian cancer (EOC). Here we report that transcription factor—Grainyhead-like 2 (GRHL2) maintains phenotype. EOC tumours with lower GRHL2 levels are associated Mes/Mesenchymal subtype and poorer overall survival. shRNA-mediated knockdown in an phenotype results EMT changes, increased...
Despite advancement in breast cancer treatment, 30% of patients with early cancers experience relapse distant metastasis. It is a challenge to identify at risk for relapse; therefore, the identification markers and therapeutic targets metastatic imperative. Here, we identified DP103 as biomarker metastasis-driving oncogene human determined that elevates matrix metallopeptidase 9 (MMP9) levels, which are associated metastasis invasion through activation NF-κB. In turn, NF-κB signaling...
Ovarian cancer (OC) can be classified into five biologically distinct molecular subgroups: epithelial-A (Epi-A), Epi-B, mesenchymal (Mes), Stem-A and Stem-B. Among them, expresses genes relating to stemness is correlated with poor clinical prognosis. In this study, we show that frizzled family receptor 7 (FZD7), a for Wnt signalling, overexpressed in the subgroup. To elucidate functional roles of FZD7, used an RNA interference gene knockdown approach three cell lines: CH1, PA1 OV-17R....
AXL inhibitors may prolong survival in a subset of patients with advanced ovarian cancer.
Abstract Metastasis is a significant health issue. The standard mode of care combination chemotherapy and targeted therapeutics but the 5-year survival rate remains low. New/better drug targets that can improve outcomes patients with metastatic disease are needed. complex process, each step conferred by set genetic aberrations. Mapping molecular changes associated metastasis improves our understanding etiology this contributes to pipeline therapeutics. Here, phosphoproteomics...
Although cancer-associated fibroblasts (CAFs) are viewed as a promising therapeutic target, the design of rational therapy has been hampered by two key obstacles. First, attempts to ablate CAFs have resulted in significant toxicity because currently used biomarkers cannot effectively distinguish activated from non-cancer associated and mesenchymal progenitor cells. Second, it is unclear whether different organs molecular functional properties that necessitate organ-specific designs. Our...
Article15 July 2020Open Access Source DataTransparent process Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity Claudia Koch Department Tumor Biology, Center Experimental Medicine, University Medical Hamburg-Eppendorf, Hamburg, Germany Search for more papers by this author Andra Kuske Simon A Joosse orcid.org/0000-0002-4296-5615 Gökhan Yigit Institute Human Genetics, Göttingen, George Sflomos orcid.org/0000-0003-2972-0549 ISREC – Swiss Cancer...
IntroductionAcquired cancer therapy resistance evolves under selection pressure of immune surveillance and favors mechanisms that promote drug through cell survival evasion. AXL receptor tyrosine kinase is a mediator phenotypic plasticity suppression tumor immunity, expression associated with diminished long-term in wide range malignancies, including NSCLC.MethodsWe aimed to investigate the underlying AXL-mediated acquired first- third-generation small molecule EGFR inhibitors (EGFRi)...