A5060869211- Analytical Chemistry and Chromatography
- Cancer Cells and Metastasis
- Surfactants and Colloidal Systems
- Mercury impact and mitigation studies
- Analytical chemistry methods development
- Heavy metals in environment
- Cancer-related molecular mechanisms research
- Ovarian cancer diagnosis and treatment
- Recycling and Waste Management Techniques
- Advanced Chemical Sensor Technologies
- Pesticide Residue Analysis and Safety
- RNA modifications and cancer
- Heavy Metal Exposure and Toxicity
- Toxic Organic Pollutants Impact
- Advanced Polymer Synthesis and Characterization
- Wnt/β-catenin signaling in development and cancer
- Mass Spectrometry Techniques and Applications
- Heavy Metals in Plants
- Extraction and Separation Processes
- Analytical Chemistry and Sensors
- Sarcoma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Cancer Genomics and Diagnostics
- Fermentation and Sensory Analysis
- Skin and Cellular Biology Research
University of Cambridge
2013-2025
KK Women's and Children's Hospital
2017-2023
National University of Singapore
1996-2018
National University Cancer Institute, Singapore
2013-2018
Bridge University
2013
The University of Melbourne
2007
Institute of Materials Research and Engineering
1999-2001
Vienna General Hospital
1992
University Clinic of Traumatology
1992
University of Southampton
1980
The phenotypic transformation of well-differentiated epithelial carcinoma into a mesenchymal-like state provides cancer cells with the ability to disseminate locally and metastasise. Different degrees epithelial–mesenchymal transition (EMT) have been found occur in carcinomas from breast, colon ovarian (OC), among others. Numerous studies focused on bona fide mesenchymal states but rarely intermediate states. In this study, we describe model system for appraising spectrum EMT using 43...
Abstract Epithelial-mesenchymal transition (EMT), a biological process by which polarized epithelial cells convert into mesenchymal phenotype, has been implicated to contribute the molecular heterogeneity of ovarian cancer (EOC). Here we report that transcription factor—Grainyhead-like 2 (GRHL2) maintains phenotype. EOC tumours with lower GRHL2 levels are associated Mes/Mesenchymal subtype and poorer overall survival. shRNA-mediated knockdown in an phenotype results EMT changes, increased...
Ovarian cancer (OC) can be classified into five biologically distinct molecular subgroups: epithelial-A (Epi-A), Epi-B, mesenchymal (Mes), Stem-A and Stem-B. Among them, expresses genes relating to stemness is correlated with poor clinical prognosis. In this study, we show that frizzled family receptor 7 (FZD7), a for Wnt signalling, overexpressed in the subgroup. To elucidate functional roles of FZD7, used an RNA interference gene knockdown approach three cell lines: CH1, PA1 OV-17R....
Clear cell sarcoma of the kidney (CCSK) is a rare paediatric renal malignant tumour. The majority CCSKs have internal tandem duplications (ITDs) BCOR gene, whereas minority YWHAE-NUTM2 gene fusion. A third 'double-negative' (DN) category comprises with neither ITDs nor aim this study was to characterise 11 histologically diagnosed immunohistochemically (with CCND1, and CCNB3 stains) genetically.By next-generation sequencing, 10 cases (90.9%) had exon 15 ITDs, positive immunoreactivity being...
Abstract Background Epithelial ovarian cancer is characterized by multiple genomic alterations; most are passenger alterations which do not confer tumor growth. Like many cancers, it a heterogeneous disease and can be broadly categorized into 4 main histotypes of clear cell, endometrioid, mucinous, serous. To date, histotype-specific copy number have been difficult to elucidate. The difficulty lies in having sufficient sample size each histotype for statistical analyses. Methods dissect the...
A modified ternary microemulsion polymerization was successfully used to prepare nanosized polystyrene (PS) microlatexes with high PS/surfactant weight ratios of up 14:1. Cationic surfactants were alkyltrimethylammonium bromides, CnTAB, where n is either 12, 14, or 16. water-soluble initiator for the at room temperature, moderate stirring. The latexes obtained are bluish-opaque and have been stable now about 8 months. process produced rather uniform (Dw/Dn ≅ 1.10) latex particles 40 nm...
Frizzled family receptor 7 (FZD7), a Wnt signaling receptor, is associated with the maintenance of stem cell properties and cancer progression. FZD7 has emerged as potential therapeutic target because it capable transducing both canonical noncanonical signals. In this study, we investigated regulatory pathway downstream its functional roles. We found that expression was crucial to mesenchymal phenotype, anoikis resistance, spheroid tumor formation in ovarian (OC). identified TWIST1 effector...
Three carcinogenic heterocyclic aromatic amines, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), and 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMelQx), have been detected by capillary zone electrophoresis in rain water accompanying suspended particulates collected during haze episodes originating from major forest fires Southeast Asia. The enveloped much of Asia, including Singapore, August through October 1994. These...
Aims We describe the clinical and pathological features novel genetic findings of a case CIC-DUX4 sarcoma occurring in thigh 35-year-old man. Methods Fusion gene detection using next-generation sequencing-based anchored multiplex PCR technique (Archer FusionPlex Sarcoma Panel) was used to identify fusion breakpoints this formalin-fixed paraffin-embedded tumour material. Results This has breakpoint between exon 20 CIC 1 DUX4 gene. Conclusions report describes an additional with breakpoint,...
Existing marker-based methods of minimal residual disease (MRD) determination in neuroblastoma do not effectively enrich for the circulating cell population. Given relative size differential tumor cells over normal hematogenous cells, we hypothesized that size-based separation could (CTCs) from blood samples and disseminated (DTCs) bone marrow aspirates (BMA) patients, their gene expression profiles vary dynamically with various states course treatment. Using a spiral microfluidic chip,...
Microemulsion polymerizations of styrene (St), butyl methacrylate (BMA), and methyl (MMA) via hollow-fiber feeding monomers have been studied for the preparation nanosized microlatexes ranging from 15 to 65 nm. A water-soluble redox initiator was used all at room temperature. The monomer-starved latex particles were first generated in initial microemulsion containing 0.5 wt % St or BMA 2.0 MMA stabilized by 1.5 SDS 1.0 CTAB. subsequent supply monomer prepolymerized proceeded external...