A5039283945- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
- Fibroblast Growth Factor Research
- RNA modifications and cancer
- Developmental Biology and Gene Regulation
- Gene expression and cancer classification
- Lung Cancer Treatments and Mutations
- Cancer, Hypoxia, and Metabolism
- Cancer Mechanisms and Therapy
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Bladder and Urothelial Cancer Treatments
- Immune cells in cancer
- 3D Printing in Biomedical Research
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Glycosylation and Glycoproteins Research
- Melanoma and MAPK Pathways
- Phagocytosis and Immune Regulation
- CAR-T cell therapy research
- Congenital heart defects research
Loewe Center for Synthetic Microbiology
2025
Centre National de la Recherche Scientifique
2007-2024
Agency for Science, Technology and Research
2015-2024
Institute of Molecular and Cell Biology
2015-2024
Guangzhou Experimental Station
2021-2024
Laboratoire Matière et Systèmes Complexes
2017-2024
Université Paris Cité
1973-2024
National University Cancer Institute, Singapore
2013-2023
National University of Singapore
2013-2023
Institut Gustave Roussy
2013-2023
Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view current methodologies precludes their systematic application analyze relatively large three-dimensional mid- late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays in situ RNA capture create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied...
Abstract Motivation: Genomic DNA regions are frequently lost or gained during tumor progression. Array Comparative Hybridization (array CGH) technology makes it possible to assess these changes in cancers, by comparison with a normal reference. The identification of systematically deleted amplified genomic set tumors enables biologists identify genes involved cancer progression because suppressor thought be located and oncogenes, regions. CGH profiles should also improve the classification...
Epithelial–mesenchymal transition (EMT) is an essential morphogenetic process during embryonic development. It can be induced in vitro by hepatocyte growth factor/scatter factor (HGF/SF), or FGF-1 our NBT-II cell model for EMT. We tested a central role EMT of zinc-finger protein called Slug. Slug mRNA and levels were increased transiently FGF-1–treated cells. Transient stable transfection cDNA cells resulted striking disappearance the desmosomal markers desmoplakin desmoglein from cell–cell...
The importance of mesenchymal-epithelial interactions for the proper development pancreas has been acknowledged since early 1960s, even though molecule(s) mediating this process have remained unknown. We demonstrate here that Fgf10, a member fibroblast growth factor family (FGFs), plays an essential role in process. show Fgf10 is expressed mesenchyme directly adjacent to dorsal and ventral pancreatic epithelial buds. In Fgf10–/– mouse embryos, evagination epithelium initial formation buds...
The aggregation of cells from dissociated neural retinas chick embryos can be inhibited by antibodies prepared against whole retinal cells. In order to identify the antigens involved, substances released tissues in culture were tested for their ability neutralize specifically inhibition antibody cell adhesion. Using this assay, three active polypeptides supernatant purified 500-fold gel filtration and polyacrylamide electrophoresis. Rabbit these activities adhesion reacted only with...
Understanding household behavior and its macro consequences for society is pivotal climate change adaptation. Yet, traditional policy decision-support models nature–society systems oversimplify human behavior. Using original modeling ...Despite the growing calls to integrate realistic in sustainability science models, representative rational agent prevails. This especially problematic adaptation that relies on actions at various scales: from ...
We describe a new method for analyzing embryonic events dependent on specific peptide recognition signal. A short, amino acid sequence in fibronectin has been implicated as site fibronectin-mediated interactions. Fibroblast adhesion to is competitively inhibited by certain synthetic peptides, including the decapeptide Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro, which appears contain cell sequence. found that this both amphibian gastrulation and avian neural crest migration vivo, well attachment...
N-CAM, the neural cell adhesion molecule, has been found at a number of regions in early (1-5 days) chicken embryo by fluorescent antibody techniques. These appear to be those concerned with induction primary developmental axis (neural plate, tube, notochord, somites) or which later inductive events occur crest cells, optic, otic, and pharyngeal placodes, cardiac mesoderm, mesonephric primordium, limb buds). The staining patterns latter group are highly dynamic transient limited epithelial...
We have used a modified, dual pipette assay to quantify the strength of cadherin-dependent cell–cell adhesion. The force required separate E-cadherin–expressing paired cells in suspension was measured as an index intercellular Separation depended on homophilic interaction functional cadherins at cell surface, increasing with duration contact and cadherin levels. Severing link between actin cytoskeleton or disrupting polymerization did not affect initiation cadherin-mediated adhesion, but...
The phenotypic transformation of well-differentiated epithelial carcinoma into a mesenchymal-like state provides cancer cells with the ability to disseminate locally and metastasise. Different degrees epithelial–mesenchymal transition (EMT) have been found occur in carcinomas from breast, colon ovarian (OC), among others. Numerous studies focused on bona fide mesenchymal states but rarely intermediate states. In this study, we describe model system for appraising spectrum EMT using 43...
Cells of the neural crest participate in a major class cell migratory events during embryonic development. From indirect evidence, it has been suggested that fibronectin (FN) might be involved these events. We have directly tested role FN adhesion and migration using several vitro model systems. Avian trunk cells adhered readily to purified plasma substrates extracellular matrices containing cellular FN. Their was inhibited by antibodies cell-binding fragment In contrast, did not adhere...