A5067328486- Cancer Genomics and Diagnostics
- Salivary Gland Tumors Diagnosis and Treatment
- Breast Lesions and Carcinomas
- Breast Cancer Treatment Studies
- Thyroid Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Ear and Head Tumors
- Cervical Cancer and HPV Research
- Cancer and Skin Lesions
- Genetic factors in colorectal cancer
- Epigenetics and DNA Methylation
- Head and Neck Anomalies
- Oral and Maxillofacial Pathology
- Neuroblastoma Research and Treatments
- Molecular Biology Techniques and Applications
- Sarcoma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Global Cancer Incidence and Screening
- Metastasis and carcinoma case studies
- AI in cancer detection
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- Head and Neck Cancer Studies
- Prostate Cancer Treatment and Research
Hôpital américain de paris
2020-2025
The University of Texas MD Anderson Cancer Center
1998-2024
Beth Israel Deaconess Medical Center
2023-2024
Massachusetts General Hospital
2015-2024
Università Cattolica del Sacro Cuore
2017-2024
Institut Gustave Roussy
2012-2023
Faculty of Media
2023
Université Paris-Saclay
2009-2023
John Wiley & Sons (United States)
2006-2021
Florida College
2019-2021
Abstract The National Cancer Institute (NCI) sponsored the NCI Thyroid Fine‐needle Aspiration (FNA) State of Science Conference on October 22–23, 2007 in Bethesda, MD. two‐day meeting was accompanied by a permanent informational website and several on‐line discussion periods between May 1 December 15, ( http://thyroidfna.cancer.gov ). This document summarizes matters regarding diagnostic terminology/classification scheme for thyroid FNA interpretation cytomorphologic criteria diagnosis...
High-throughput genomic analyses may improve outcomes in patients with advanced cancers. MOSCATO 01 is a prospective clinical trial evaluating the benefit of this approach. Nucleic acids were extracted from fresh-frozen tumor biopsies and analyzed by array comparative hybridization, next-generation sequencing, RNA sequencing. The primary objective was to evaluate as measured percentage presenting progression-free survival (PFS) on matched therapy (PFS2) 1.3-fold longer than PFS prior (PFS1)....
Circulating tumour cells (CTCs) can provide information on patient prognosis and treatment efficacy. However, there is no universal method to detect CTC currently available. Here, we compared the performance of two detection systems based expression EpCAM antigen (CellSearch assay) or cell size (ISET assay). were enumerated in 60 patients with metastatic carcinomas breast, prostate lung origins using CellSearch according manufacturer's protocol ISET by studying cytomorphology immunolabelling...
Circulating tumour cells (CTC) have a crucial role in metastasis formation and can consistently provide information on patient prognosis. Epithelial-mesenchymal transition (EMT) is considered as an essential process the metastatic cascade, but there currently very few data demonstrating directly existence of EMT CTCs.CTCs were enriched by blood filtration using ISET (isolation size epithelial cells), triply labelled with fluorescent anti-vimentin, anti-pan-keratin antibodies SYTOX orange...
We propose a unique method for cell sorting, “Ephesia,” using columns of biofunctionalized superparamagnetic beads self-assembled in microfluidic channel onto an array magnetic traps prepared by microcontact printing. It combines the advantages notably application well controlled, flow-activated interaction between cells and beads, those immunomagnetic use batch-prepared, characterized antibody-bearing beads. On lines mixtures, we demonstrated capture yield better than 94%, possibility to...
Pathologic TNM staging is currently the best prognostic factor for non-small cell lung carcinoma (NSCLC). However, even in early-stage NSCLC, recurrence rates after surgery range from 25% to 50%. The preoperative detection of circulating tumor cells (CTC) could be useful tailor new therapeutic strategies NSCLC. We assessed presence CTC NSCLC patients undergoing surgery, using cytologic analyses, their isolation by size epithelial (ISET method). and number CTCs were considered correlated with...
Abstract Comparison of the efficacy different enrichment methods for detection circulating tumor cells (CTCs) before radical surgery is lacking in non‐small‐cell lung carcinoma (NSCLC) patients. Detection and enumeration CTCs 210 consecutive patients undergoing NSCLC were evaluated with CellSearch Assay™ (CS), using Epithelial Cell Kit, by isolation size epithelial (ISET) method, double immunolabeling anti‐cytokeratin anti‐vimentin antibodies. detected 144 (69%) CS and/or ISET 104 (50%) 82...
Epithelial-to-mesenchymal transition (EMT) mediates cancer cell invasion, metastasis, and drug resistance, but its impact on immune surveillance has not been explored. In this study, we investigated the functional consequences of mode epithelial plasticity targeted lysis by cytotoxic T lymphocytes (CTL). Acquisition EMT phenotype in various derivatives MCF-7 human breast cells was associated with dramatic morphologic changes actin cytoskeleton remodeling, CD24(-)/CD44(+)/ALDH(+) stem...
Purpose The diagnostic test for ALK rearrangement in non–small-cell lung cancer (NSCLC) crizotinib treatment is currently done on tumor biopsies or fine-needle aspirations. We evaluated whether diagnosis could be performed by using circulating cells (CTCs). Patients and Methods presence of an was examined CTCs 18 ALK-positive 14 ALK-negative patients a filtration enrichment technique filter-adapted fluorescent situ hybridization (FA-FISH), FISH method optimized filters. ALK-rearrangement...
An international panel of experts in the field salivary gland cytology (SGC), supported by American Society Cytopathology (ASC) and International Academy Cytology, conducted a survey to seek evidence practice patterns regarding SGC. Results were used provide focus for proposed Milan System Reporting Salivary Gland Cytopathology.The study group, formed during 2015 European Congress Cytology held Milan, Italy, generated that included 49 specific questions related taxonomies, practices,...
Since the publication of first edition in 2010, The Bethesda System for Reporting Thyroid Cytopathology has allowed cytopathologists to use a standardized, category-based reporting system thyroid fine needle aspirations. third builds on success 2 earlier editions and offers several key updates. most important is assignment single name each 6 diagnostic categories: (i) nondiagnostic; (ii) benign; (iii) atypia undetermined significance; (iv) follicular neoplasm; (v) suspicious malignancy; (vi)...
PURPOSE: To determine the incidence and prognostic significance of eradication cytologically proven axillary lymph node metastases in breast cancer patients treated with primary chemotherapy. PATIENTS AND METHODS: Between January 1985 December 1994, 152 invasive T1 to T3 tumors by fine-needle sampling underwent chemotherapy followed lumpectomy or mastectomy, level I II dissection, irradiation. We studied pathologic complete responses (pCRs) nodes tumors, as well predictors distant...
Microscopic extrascleral involvement and of optic nerve resection line are accepted risk factors for orbital and/or metastatic disease from retinoblastoma. Conversely, choroidal retrolaminar questionable factors. The aim this retrospective study was to define the histopathologic in patients treated by first-line enucleation.Histopathologic review 172 evaluable at Institut Curie between 1977 1990 determined degree (minimal or massive), scleral (intra- extrascleral), (prelaminar, with without...
Low doses of the alkylating agent cyclophosphamide (CTX) mediate antiangiogenic and immunostimulatory effects, leading to potent tumoricidal activity in association with various immunotherapeutic strategies. Here, we show rodents cancer patients that CTX markedly promotes differentiation CD4(+) T helper 17 (Th17) cells can be recovered both blood tumor beds. However, does not convert regulatory into Th17 cells. Because are inducers tissue inflammation autoimmunity, these results suggest...
Recurrence rates after surgery for non-small cell lung cancer (NSCLC) range from 25 to 50% and 5-year survival is only 60-70%. Because no biomarkers are predictive of recurrence or the onset metastasis, pathological TNM (pTNM) staging currently best prognostic factor. Consequently, preoperative detection circulating tumour cells (CTCs) might be useful in tailoring therapy. The aim this study was characterize morphologically any non-haematological (CNHCs) patients undergoing NSCLC using...
Detection of circulating tumor cells (CTCs) morphologically may be a promising new approach in clinical oncology. We tested the reliability cytomorphologic to identify CTCs: 808 blood samples from patients with benign and malignant diseases healthy volunteers were examined using isolation by size epithelial cell (ISET) method. Cells having nonhematologic features (so-called [CNHCs]) classified into 3 categories: CNHCs features, uncertain features. found 11.1% 48.9% nonmalignant pathologies,...
Abstract Purpose: This single-institutional feasibility study prospectively characterized genomic alterations in recurrent or refractory solid tumors of pediatric patients to select a targeted therapy. Experimental Design: Following treatment failure, with signed consent and ages above 6 months, underwent tumor biopsy surgical resection primary metastatic site. These newly acquired samples were analyzed by comparative hybridization array, next-generation sequencing for 75 target genes,...
Genetic aberrations affecting the c-ros oncogene 1 (ROS1) tyrosine kinase gene have been reported in a small subset of patients with non-small-cell lung cancer (NSCLC). We evaluated whether ROS1-chromosomal rearrangements could be detected circulating tumor cells (CTCs) and examined heterogeneity CTCs biopsies ROS1-rearranged NSCLC patients.Using isolation by size epithelial (ISET) filtration filter-adapted-fluorescence situ hybridization (FA-FISH), ROS1 rearrangement was from four treated...