A5088972365- Cancer-related molecular mechanisms research
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- PI3K/AKT/mTOR signaling in cancer
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Sphingolipid Metabolism and Signaling
- Endoplasmic Reticulum Stress and Disease
- Cell Adhesion Molecules Research
- Lymphoma Diagnosis and Treatment
- Artificial Intelligence in Healthcare and Education
- DNA Repair Mechanisms
- COVID-19 diagnosis using AI
- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Glioma Diagnosis and Treatment
- Calcium signaling and nucleotide metabolism
National University of Singapore
2016-2025
National University Cancer Institute, Singapore
2014-2025
National University Health System
2016-2025
Taipei Medical University
2025
Duke-NUS Medical School
2016
Agency for Science, Technology and Research
2016
Institute of Molecular and Cell Biology
2016
Semmelweis University
2016
University of North Texas
2016
University of South Australia
2016
Objective An unmet need exists for a non-invasive biomarker assay to aid gastric cancer diagnosis. We aimed develop serum microRNA (miRNA) panel identifying patients with all stages of from high-risk population. Design conducted three-phase, multicentre study comprising 5248 subjects Singapore and Korea. Biomarker discovery verification phases were done through comprehensive miRNA profiling multivariant analysis 578 candidates in retrospective cohorts 682 subjects. A clinical was developed...
Mitophagy is an important type of selective autophagy for specific elimination damaged mitochondria. PTEN-induced putative kinase protein 1 (PINK1)-catalyzed phosphorylation ubiquitin (Ub) plays a critical role in the onset PINK1–Parkin-mediated mitophagy. Phosphatase and tensin homolog (PTEN)-long (PTEN-L) newly identified isoform PTEN, with addition 173 amino acids to its N-terminus. Here we report that PTEN-L novel negative regulator mitophagy via phosphatase activity against...
During sepsis, activation of phagocytes leads to the overproduction proinflammatory cytokines, causing systemic inflammation. Despite substantial information regarding underlying molecular mechanisms that lead several elements in pathway remain be elucidated. We found enzyme sphingosine kinase 1 (SphK1) is up-regulated stimulated human and peritoneal patients with severe sepsis. Blockade SphK1 inhibited phagocyte production endotoxin-induced cytokines. observed protection against sepsis mice...
Despite advancement in breast cancer treatment, 30% of patients with early cancers experience relapse distant metastasis. It is a challenge to identify at risk for relapse; therefore, the identification markers and therapeutic targets metastatic imperative. Here, we identified DP103 as biomarker metastasis-driving oncogene human determined that elevates matrix metallopeptidase 9 (MMP9) levels, which are associated metastasis invasion through activation NF-κB. In turn, NF-κB signaling...
Histone deacetylases (HDACs) are enzymes involved in transcriptional repression. We aimed to examine the significance of HDAC1 and HDAC2 gene expression prediction recurrence survival 156 patients with hepatocellular carcinoma (HCC) among a South East Asian population who underwent curative surgical resection Singapore. found that were upregulated majority HCC tissues. The presence tumor tissues was correlated poor differentiation. Notably, adjacent non-tumor hepatic satellite nodules...
Triple-negative breast cancer (TNBC) is characterized by unique aggressive behavior and lack of targeted therapies. Among the various molecular subtypes cancer, it was observed that TNBCs express elevated levels sphingosine kinase 1 (SPHK1) compared to other tumor subtypes. High SPHK1 gene expression correlated with poor overall progression- free survival, as well response Doxorubicin-based treatment. Inhibition found attenuate ERK1/2 AKT signaling reduce growth TNBC cells in vitro a...
Generative AI will become an integral part of education in future. The potential this technology different disciplines should be identified to promote effective adoption. This study evaluated the performance ChatGPT tutorial and case-based learning questions physiology biochemistry for medical undergraduates. Our mainly focused on GPT-3.5 version while a subgroup was comparatively assessed GPT-4 performances.
Gelsolin is a cytoskeletal protein which participates in actin filament dynamics and promotes cell motility plasticity. Although initially regarded as tumor suppressor, gelsolin expression certain tumors correlates with poor prognosis therapy-resistance. In vitro, has anti-apoptotic pro-migratory functions critical for invasion of some types cells. We found that was highly expressed at borders infiltrating into adjacent liver tissues, examined by immunohistochemistry. contributes to...
Epithelial-mesenchymal transition (EMT) is characterized by the acquisition of invasive fibroblast-like morphology epithelial cells that are highly polarized. EMT recognized as a crucial mechanism in cancer progression and metastasis. In this study, we sought to assess involvement manganese superoxide dismutase (MnSOD) during switch between epithelial-like mesenchymal-like phenotypes breast carcinoma.Analysis carcinomas from The Cancer Genome Atlas database revealed strong positive...
The invasive behaviour of tumour cells has been attributed in part to dysregulated cell motility. Members the ADF/Cofilin family actin-binding proteins are known increase microfilament dynamics by increasing rate at which actin monomers leave pointed end filament and a filament-severing activity. As depolymerisation is rate-limiting step dynamics, ADF/Cofilins suspected facilitate motility cells. To test this, we investigated influence cofilin on transient stably overexpressing human...
One partner of a fusion gene found in gastric cancer, CD44-SLC1A2 , may contribute to the tumor’s abnormal metabolism.
Abstract Antibody-drug conjugates (ADCs) are "biological missiles" that utilize a chemical linker to connect monoclonal antibodies (mAbs) with cytotoxic drugs, which induces the precise killing of targeted tumor cells and causes death surrounding untargeted cancer through bystander effect. However, current ADCs too specific certain subtypes not enough accurately target cancer-specific protein, limits therapeutic populations unwanted side effects. We developed heavy-chain antibody (HCAb) from...