A5041474561- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Plant biochemistry and biosynthesis
- Circular RNAs in diseases
- Microbial Metabolic Engineering and Bioproduction
- Molecular Biology Techniques and Applications
- Helicobacter pylori-related gastroenterology studies
- Microbial Natural Products and Biosynthesis
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Radiomics and Machine Learning in Medical Imaging
- Metabolomics and Mass Spectrometry Studies
- Glioma Diagnosis and Treatment
- Cardiovascular and exercise physiology
- Molecular Communication and Nanonetworks
- Ferroptosis and cancer prognosis
- Heart Failure Treatment and Management
- Biochemical and biochemical processes
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Gastric Cancer Management and Outcomes
- Genetic Associations and Epidemiology
Shanghai Institute of Hypertension
2025
Shanghai Jiao Tong University
2025
Ruijin Hospital
2025
Ningbo University
2024
AbMax AntibodyChina (China)
2024
Agency for Science, Technology and Research
2014-2020
Bioprocessing Technology Institute
2014-2020
Singapore-MIT Alliance for Research and Technology
2011-2017
Tianjin University
2010
Objective An unmet need exists for a non-invasive biomarker assay to aid gastric cancer diagnosis. We aimed develop serum microRNA (miRNA) panel identifying patients with all stages of from high-risk population. Design conducted three-phase, multicentre study comprising 5248 subjects Singapore and Korea. Biomarker discovery verification phases were done through comprehensive miRNA profiling multivariant analysis 578 candidates in retrospective cohorts 682 subjects. A clinical was developed...
Significance Lung cancer is the most prevalent and deadly worldwide. Minimally invasive testing for early detection of lung key improving prognosis. In this study, we carried out a multicenter, multiethnic study to compare serum miRNA profiles between 744 NSCLC 944 healthy controls. We discovered 35 candidate biomarkers, verified 22 them, panel 5 miRNAs was optimized stage with AUC > 0.90 in three independent validation cohorts. The sensitivity specificity five-miR I were 83.0% 90.7%,...
Isoprenoids are natural products that all derived from isopentenyl diphosphate (IPP) and dimethylallyl (DMAPP). These precursors synthesized either by the mevalonate (MVA) pathway or 1-Deoxy-D-Xylulose 5-Phosphate (DXP) pathway. Metabolic engineering of microbes has enabled overproduction various isoprenoid DXP including lycopene, artemisinic acid, taxadiene levopimaradiene. To date, there is no method to accurately measure metabolic intermediates simultaneously so as enable identification...
Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is promising microbial host this purpose because its fast growth rate and GRAS (generally regarded safe) status. To date, development has impaired by lack genetic tools modulating expression multiple genes. In study, we present novel two-promoter system which can be used to independently control levels two gene cassettes over large dynamic range....
Aberrant miRNA expression has been associated with many diseases, and extracellular miRNAs that circulate in the bloodstream are remarkably stable. Recently, there growing interest identifying cell-free circulating can serve as non-invasive biomarkers for early detection of disease or selection treatment options. However, quantifying levels biofluids is technically challenging due to their low abundance. Using reference samples, we performed a cross-platform evaluation which profiling was on...
Abstract Background Mammography is widely used for breast cancer screening but suffers from a high false-positive rate. Here, we perform the largest comprehensive, multi-center study to date involving diverse ethnic groups, identification of circulating miRNAs screening. Methods This had discovery phase ( n = 289) and two validation phases 374 379). Quantitative PCR profiling 324 was performed on serum samples (all stages) healthy subjects identify miRNA biomarkers. Two-fold cross-validation...
Isoprenoids are a large and diverse class of compounds that includes many high value natural products thus in great demand. To meet the increasing demand for isoprenoid compounds, metabolic engineering microbes has been used to produce isoprenoids an economical sustainable manner. achieve yields using this technology, availability precursors feeding deoxyxylulose phosphate (DXP) pathway, responsible biosynthesis, be optimized. In study, phosphoenolpyruvate, vital DXP pathway precursor, was...
The ability to assemble multiple fragments of DNA into a plasmid in single step is invaluable studies metabolic engineering and synthetic biology. Using phosphorothioate chemistry for high efficiency site specific cleavage sequences, novel ligase independent cloning method (cross-lapping vitro assembly, CLIVA) was systematically rationally optimized E. coli. A series 16 constructs combinatorially expressing genes encoding enzymes the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway were...
Objective The aim of this study was to evaluate the accuracy CONTEC08A oscillometric upper-arm blood pressure (BP) monitor for BP measurement in adults according International Organization Standardization (ISO) 81060-2:2018 and amendment (Amd)1:2020 standard. Methods Eighty-five subjects (male 40 female 45) were recruited fulfill age, sex, BP, cuff distribution criteria ISO standard general population, had a mean age 40.2 years. same arm sequential method used with three differently sized...
Microbial ecosystems have been widely used in industrial production, but the inter-relationships of organisms within them haven't completely clarified due to complex composition and structure natural microbial ecosystems. So it is challenging for ecologists get deep insights on how function interplay with surrounding environments. But recent progresses synthetic biology show that construction artificial where relationships species are comparatively clear could help us further uncover meadow...
In vitro synthesis of chemicals and pharmaceuticals using enzymes is considerable interest as these biocatalysts facilitate a wide variety reactions under mild conditions with excellent regio-, chemo- stereoselectivities. A significant challenge in multi-enzymatic reaction the need to optimize various steps involved simultaneously so obtain high-yield product. this study, statistical experimental design was used guide optimization total amorpha-4,11-diene (AD) multienzymes mevalonate...
Abstract The Singapore Integrative Omics Study provides valuable insights on establishing population reference measurement in 364 Chinese, Malay, and Indian individuals. These measurements include > 2.5 millions genetic variants, 21,649 transcripts expression, 282 lipid species quantification, 284 clinical, lifestyle, dietary variables. This concept paper introduces the depth of data resource, investigates extent ethnic variation at these omics non-omics biomarkers. It is evident that...
Recombinant proteins are routinely overexpressed in metabolic engineering. It is well known that some over-expressed heterologous recombinant enzymes insoluble with little or no enzymatic activity. This study examined the solubility of homologous deoxyxylulose phosphate pathway (DXP) and impact inclusion body formation on engineering microbes.Four this (DXS, ISPG, ISPH ISPA), but not all, were highly insoluble, regardless expression systems used. Insoluble dxs (the committed enzyme DXP...
As miRNAs emerge as potential circulating biomarkers for the diagnosis or prognosis of a wide variety diseases, quantification miRNA necessitates careful preanalytic considerations and sample quality control becomes crucial. This study comprehensively analyzed profiles 356 by quantitative RT-PCR in various blood types, with processing protocols. The comprehensive analysis investigated correlations individual certain confounding factors. On basis these profiles, panel 7 was established...
In vitro metabolic engineering is an alternative approach to cell-based biosynthesis. It offers unprecedented flexibility for the study of biochemical pathways, thus providing useful information rational design and assembly reaction modules. Herein, we took advantage in initially gain insight into regulatory network a reconstituted amorpha-4,11-diene (AD) synthetic pathway. Guided by lin-log approximation, rapidly identified hitherto unrecognized inhibition adenosine triphosphate (ATP) on...
Mammography is extensively used for breast cancer screening but has high false-positive rates. Here, prospectively collected blood samples were to identify circulating microRNA (miRNA) biomarkers discriminate between malignant and benign lesions among women with abnormal mammograms. The Discovery cohort comprised 72 patients 197 lesions, while the Validation had 73 196 cases, respectively. Absolute expression levels of 324 miRNAs determined using RT-qPCR. miRNA biomarker panels identified...
Abstract Background Germinomas (IG) account for up to 50% of all intracranial germ cell tumours. These tumours are reputed be more prevalent in Oriental populations comparison Western cohorts. Biological characteristics IG other ethnic groups unknown. Singapore is a multi-ethnic country with diverse cultures. Owing inter-racial heterogeneity, the authors hypothesize there molecular differences between paediatric patients our local population. The aims this study exploratory: firstly,...