A5100330326- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Toxoplasma gondii Research Studies
- Pancreatic and Hepatic Oncology Research
- Cytomegalovirus and herpesvirus research
- Lung Cancer Diagnosis and Treatment
- Nerve injury and regeneration
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Neuroendocrine Tumor Research Advances
- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Lung Cancer Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Valve Diseases and Treatments
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- Cellular Mechanics and Interactions
- Nanofabrication and Lithography Techniques
- RNA Interference and Gene Delivery
- Pediatric Urology and Nephrology Studies
Xuzhou Medical College
2018-2025
Inner Mongolia Electric Power (China)
2024
Gold Coast Hospital
2021-2024
Fujian Provincial Hospital
2024
Shantou University
2024
Merck (Singapore)
2019-2024
Nanjing Medical University
2020-2024
Zhejiang University
2012-2024
The Fourth People's Hospital
2024
Chongqing University
2024
Cell-free circulating tumor DNA (ctDNA) in plasma has been used as a potential noninvasive biomarker for various tumors. Our study was performed to evaluate the clinical implications of ctDNA detection patients with metastatic pancreatic cancer. First, we attempted prospectively screen panel 60 genes cell-free (cfDNA) from ten cancer via exome sequencing. Second, droplet digital PCR (ddPCR) identify mutations cohort 188 Finally, preliminary role monitoring responses following chemotherapy,...
Abstract The detection of circulating tumor cells (CTCs) is crucial to early cancer diagnosis and the evaluation metastasis. However, it remains challenging due scarcity CTCs in blood. Herein, we report an ultrasensitive platform for direct using luminescent lanthanide nanoprobes. These were designed recognize epithelial cell adhesion molecules on cells, allowing signal amplification through dissolution‐enhanced time‐resolved photoluminescence (TRPL) elimination short‐lived autofluorescence...
Metastasis is responsible for at least 90% of colon cancer (CC)-related deaths. Lipid metabolism a critical factor in metastasis, yet the underlying mechanism requires further investigation. Herein, through utilisation single-cell sequencing and proteomics, we identified sulfotransferase SULT2B1 as novel metastatic tumour marker CC, which was associated with poor prognosis. CC orthotopic model vitro assays showed that promoted lipid metastasis. Moreover, directly interacted SCD1 to...
Inhibition of protein neddylation, particularly cullin has emerged as a promising anticancer strategy, evidenced by the antitumor activity in preclinical studies Nedd8-activating enzyme (NAE) inhibitor MLN4924. This small molecule can block neddylation pathway and is now clinical trials. We others have previously shown that MLN4924 mediated its ability to induce apoptosis, autophagy senescence cell context-dependent manner. However, whether any effect on tumor angiogenesis remains...
Background Chronic infection with the neurotropic parasite Toxoplasma gondii (T. gondii) can cause anxiety and gut microbiota dysbiosis in hosts. However, potential role of induced by remains unclear.
Activation of heterotrimeric GTP-binding (G) proteins by their coupled receptors, causes dissociation the G protein α and βγ subunits. Gβγ subunits interact directly with protein-gated inwardly rectifying K+ (GIRK) channels to stimulate activity. In addition, free subunits, resulting from agonist-independent can account for a major component basal channel activity.Using series chimeric constructs between GIRK4 Gβγ-insensitive channel, IRK1, we have identified critical site interaction GIRK...
ABSTRACT 16S rRNA sequence data have been used to provide a molecular basis for an accurate system identification of members the genus Mycobacterium . Previous studies shown that species demonstrate high levels (>94%) similarity and this method cannot differentiate between all species, i.e., M. gastri kansasii In present study, we recA gene as alternative sequencing target in order complement sequence-based genetic identification. The genes 30 were amplified by PCR, sequenced, compared...
Metal assisted chemical etching (MACE) of Si has been used to fabricate both simple and complex nanostructures, through the relatively straightforward process noble metal deposition patterning followed by immersion in a suitable solution. Under appropriate conditions, is catalyzed occurs only at metal–silicon interface. MACE therefore requires that force be present keeps silicon close proximity during etching. The geometrical characteristics etched nanostructures depend not on solution...
Pancreatic cancer is characterized by extremely poor prognosis because of early recurrence and metastasis, increasing evidence supports the critical role microRNA in progression. Here we identified that microRNA-34b functioned as a tumor-suppressing targeting oncogenic Smad3 pancreatic cancer. As hypovascular tumor with potential endoplasmic reticulum stress microenvironment, miR-34b was silenced after ER inducer thapsigargin (Tg) treatment negatively regulated chaperone glucose protein 78...
Accumulating evidence indicates that microRNAs (miRNAs) have great potential as tumor biomarkers and therapeutic agents owing to their functions in tumorigenesis cancer progression. Aberrant expression of miR-1247 has been found several cancers is predicted play an important role the pathological processes pancreatic by miRNA-regulated network analysis. We investigated profile tissue microarray situ hybridization was significantly down-regulated tissues compared matched benign tissues. High...
The Lewis ( FUT 3 ) and Secretor 2 genes, corresponding to secretion of ABO (H) histo‐blood group antigen CA 19‐9, are highly polymorphic with differences between populations. In this study, the genes in 316 Chinese participants were sequenced detect polymorphisms, associated 19‐9 was also measured. total, 14 genotypes 10 verified. Le / , le 59,508 59 main frequencies 53.2%, 10.7% 3.5%, respectively. Se se 385 21.4%, 18.6% 16.2%, alleles 1067 508 found extensively population, frequency...
Abstract Gemcitabine (GEM) is the standard chemotherapy drug for pancreatic cancer. Because of widespread resistance, effect limited. Therefore, it urgent to reveal underlying mechanism. Glycolysis most remarkable character tumor aberrant metabolism, which plays vital roles on resistance. Hexokinase 2 (HK2), as key enzyme regulating first‐step reaction glycolysis, overexpressed in many kinds tumors. The putative role HK2 resisting GEM therapy was investigated this study. We found that cancer...
Toxoplasma gondii (T. gondii) induces gut barrier integrity impairment, which is crucial to the establishment of long-term infection in hosts. Cellular senescence an imperative event that drives disease progression. Several studies have indicated T. oxidative stress and cell cycle blockade tissues hosts, suggesting cellular induced by parasite. Here, we explored whether involved gondii-mediated colonic damage mice. C57BL/6J mice were infected with 10 cysts gondii. Senolytic therapy...
The folding ability of chromatin fractions containing approximately identical nucleosome numbers and the same linker histone composition, but with different extents core acetylation, were analyzed by analytical ultracentrifugation. It was found that acetylated consistently exhibited a relatively small significantly lower extent compaction than their native nonacetylated counterparts. This regardless size distribution heterogeneity analyzed. Furthermore fibers an enhanced solubility in both...
Abstract The Singapore Integrative Omics Study provides valuable insights on establishing population reference measurement in 364 Chinese, Malay, and Indian individuals. These measurements include > 2.5 millions genetic variants, 21,649 transcripts expression, 282 lipid species quantification, 284 clinical, lifestyle, dietary variables. This concept paper introduces the depth of data resource, investigates extent ethnic variation at these omics non-omics biomarkers. It is evident that...
Abstract The actin‐binding protein profilin1 (PFN1) plays a central role in actin dynamics, which is essential for cytotoxic T lymphocyte (CTL) functions. functional of PFN1 CTLs, however still remains elusive. Here, we identify as the only member profilin family expressed primary human CD8 + cells. Using vitro assays, find that negative regulator CTL‐mediated elimination target Furthermore, involved activation‐induced lytic granule (LG) release, CTL migration and modulation structures at...
Background: Direct reprogramming of astrocytes into neurons opens up a new avenue for neuroregenerative medicine. However, the poor understanding molecular mechanisms underpinning latent neurogenic program in has largely restricted this strategy towards safe and effective clinical therapies. Methods: Immunocytochemistry, immunohistochemistry, western blotting, qRT-PCR, gene knockdown fate-mapping are performed to analyze role NOTCH1 signaling regulation reactive after spinal cord injury....