A5100462435- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Nicotinic Acetylcholine Receptors Study
- Neurotransmitter Receptor Influence on Behavior
- Anesthesia and Neurotoxicity Research
- Ion Transport and Channel Regulation
- Hearing, Cochlea, Tinnitus, Genetics
- Biochemical Analysis and Sensing Techniques
- Neuroscience and Neural Engineering
- Sleep and Wakefulness Research
- Neurobiology and Insect Physiology Research
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Atherosclerosis and Cardiovascular Diseases
- Seismic Waves and Analysis
- Protein Kinase Regulation and GTPase Signaling
- Earthquake Detection and Analysis
- Nerve injury and regeneration
- Ionosphere and magnetosphere dynamics
- Mitochondrial Function and Pathology
Hebei Medical University
2016-2025
Ministry of Education of the People's Republic of China
2013-2025
China University of Petroleum, East China
2025
Anhui Medical University
2022-2024
First Affiliated Hospital of Hebei Medical University
2023-2024
Unilever (China)
2024
Yancheng Third People's Hospital
2024
Nanjing Medical University
2024
Nantong University
2024
Xintai People's Hospital
2023
Bradykinin (BK) is an inflammatory mediator and one of the most potent endogenous pain-inducing substances. When released at sites tissue damage or inflammation, applied exogenously, BK produces acute spontaneous pain causes hyperalgesia (increased sensitivity to potentially painful stimuli). The mechanisms underlying induced by are poorly understood. Here we report that in small nociceptive neurons from rat dorsal root ganglia, BK, acting through its B2 receptors, PLC, release calcium...
The integration of somatosensory information is generally assumed to be a function the central nervous system (CNS). Here we describe fully functional GABAergic communication within rodent peripheral sensory ganglia and show that it can modulate transmission pain-related signals from nerves CNS. We found neurons express major proteins necessary for GABA synthesis release released in response depolarization. In vivo focal infusion or reuptake inhibitor dramatically reduced acute peripherally...
We identified major ion channels influencing the resting membrane potential of nociceptive sensory neurons and demonstrated that changes somatic/perisomatic these can strongly influence peripheral transmission. Peripheral ganglia contain somata afferent fibres conveying somatosensory inputs to central nervous system. Growing evidence suggests region Control (Erest) is an important mechanism regulating excitability, but surprisingly little known about how Erest regulated in neuron or affect...
Accumulating observations suggest that peripheral somatosensory ganglia may regulate nociceptive transmission, yet direct evidence is sparse. Here, in experiments on rats and mice, we show the afferent information undergoes dynamic filtering within dorsal root ganglion (DRG) this occurs at axonal bifurcations (t-junctions). Using synchronous vivo electrophysiological recordings from central processes of sensory neurons (in spinal nerve root), ganglionic transplantation GABAergic progenitor...
Abstract Calcium‐activated chloride channels (CaCCs) are ion with key roles in physiological processes. They abnormally expressed various cancers, including esophageal squamous cell cancer, head and neck carcinoma, colorectal gastrointestinal stromal tumors. The CaCC component TMEM16A/ANO1 was recently shown to be overexpressed lung adenocarcinoma cells may serve as a tumorigenic protein. In this study, we determined that matrine is potent TMEM16A inhibitor exerts anti‐lung effects. Patch...
Small interfering RNA (siRNA) is utilized as a potent agent for cancer therapy through regulating the expression of genes associated with tumors. While widely application siRNAs in treatment severely limited by their insufficient biological stability and its poor ability to penetrate cell membranes. Targeted delivery systems hold great promise selectively deliver loaded drug tumor site reduce toxic side effect. However, elevated interstitial fluid pressure efficient cytoplasmic release are...
The transmembrane channel-like (TMC) protein family comprises eight members, with TMC1 and TMC2 being extensively studied. This study demonstrates substantial co-expression of TMC7 the mechanosensitive channel Piezo2 in somatosensory neurons. Genetic deletion primary sensory ganglia neurons vivo enhances sensitivity both physiological pathological mechanosensory transduction. leads to an increase proportion rapidly adapting (RA) currents conducted by dorsal root ganglion (DRG) accelerates RA...
Activation of heterotrimeric GTP-binding (G) proteins by their coupled receptors, causes dissociation the G protein α and βγ subunits. Gβγ subunits interact directly with protein-gated inwardly rectifying K+ (GIRK) channels to stimulate activity. In addition, free subunits, resulting from agonist-independent can account for a major component basal channel activity.Using series chimeric constructs between GIRK4 Gβγ-insensitive channel, IRK1, we have identified critical site interaction GIRK...
Vascular smooth muscle cells (VSMCs) can switch between differentiated and dedifferentiated phenotypes, this phenotype is believed to be essential for repair of vascular injury. We studied the inhibitory effect 22 alpha (SM22 alpha) on VSMC proliferation in vitro vivo explored potential molecular mechanisms effect.By using coimmunoprecipitation glutathione S-transferase pull-down assays, we have shown that SM22 binds Ras alpha-overexpressed VSMCs presence or absence platelet-derived growth...
Depression symptoms are often found in patients suffering from chronic pain, a phenomenon that is yet to be understood mechanistically. Here, we systematically investigate the cellular mechanisms and circuits underlying chronic-pain-induced depression behavior. We show development of pain accompanied by depressive-like behaviors mouse model trigeminal neuralgia. In parallel, observe increased activity dopaminergic (DA) neuron midbrain ventral tegmental area (VTA), inhibition this elevated...
G protein-sensitive inwardly rectifying potassium (GIRK) channels are activated through direct interactions of their cytoplasmic N- and C-terminal domains with the βγ subunits proteins. By using a combination biochemical electrophysiological approaches, we identified minimal Gβγ-binding responsible for stimulation GIRK4 channel activity. Within these one N-terminal residue, His-64, Leu-268, proved critical Gβγ-mediated Moreover, mutations at sites reduced significantly binding to Gβγ. The...
Confocal laser scanning microscopy (CLSM) and whole-cell patch-clamp were used to investigate the role of Ca2+ influx in maintaining cytosolic concentration ([Ca2+]c) features pathway germinating pollen grains Lilium davidii D. [Ca2+]c decreased when was inhibited by EGTA or channel blockers. A hyperpolarization-activated Ca2+-permeable channel, which can be suppressed trivalent cations, verapamil, nifedipine diltiazem, identified on plasma membrane protoplasts with recording. Calmodulin...
Rabies virus (RABV) invades the central nervous system and nearly always causes fatal disease in humans. RABV enters cells via clathrin-mediated endocytosis upon receptor binding. The detailed mechanism of this process how it is regulated are not fully understood. Here, we carried out a high-through-put RNAi analysis identified AP2-associated kinase 1 (AAK1), serine/threonine kinase, as an important cellular component regulating entry RABV. AAK1 knock-down greatly inhibits infection cells,...
Fibroblast growth factor homologous factors (FHFs), among other fibroblast factors, are increasingly found to be important regulators of ion channel functions. Although FHFs have been link several neuronal diseases and arrhythmia, its role in inflammatory pain still remains unclear. This study aimed investigate the mechanism FGF13 pain. Fgf13 conditional knockout mice were generated CFA-induced chronic model was established measure threshold. Immunostaining, western blot quantitative...
Abstract Considering recent breakthroughs in the field of optogenetics, a powerful tool is established present study to modulate activities target neurons through application light‐based methods. Near‐infrared (NIR) light enables penetration deep‐tissue. As result, it can be used functions proteins/cells. Herein, aimed develop NIR light‐sensitive drug delivery system spatially and temporally control activation loaded at stimulation sites its release from nanoparticle sensitive NIR. Owing...
Lung cancer has the highest mortality among cancers worldwide due to its high incidence and lack of effective cures. We have previously demonstrated that membrane ion channel TMEM16A is a potential drug target for treatment lung adenocarcinoma identified pocket inhibitor binding provides basis screening promising new inhibitors. However, conventional discovery strategies are lengthy costly, unpredictable side effects lead failure rate in development. Therefore, finding therapeutic directions...
Prior studies indicate that applying low-intensity transcranial ultrasound stimulation (TUS) to the hippocampus can suppress epileptic seizures. Nevertheless, it is unclear how TUS regulates hippocampal neural activity, and whether discharges in motor cortex are suppressed by modulating activity. To explore answers of above questions, was utilized investigate responses aforementioned inquiries stimulating mice with penicillin-induced epilepsy, while simultaneously recording local field...