Xiaowen Feng
A5000405667- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- Chromosomal and Genetic Variations
- Advanced Power Amplifier Design
- Radio Frequency Integrated Circuit Design
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Machine Learning in Bioinformatics
- Genomic variations and chromosomal abnormalities
- Immunotherapy and Immune Responses
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Parallel Computing and Optimization Techniques
- Genetic diversity and population structure
- Molecular Sensors and Ion Detection
- Algorithms and Data Compression
- Biochemical effects in animals
- Lipid metabolism and disorders
- Lipid metabolism and biosynthesis
- Advanced DC-DC Converters
- Acute Ischemic Stroke Management
Anhui Medical University
2021-2024
Ministry of Education of the People's Republic of China
2011-2024
Dana-Farber Cancer Institute
2020-2024
Harvard University
2017-2024
Lanzhou University
2011-2023
Lanzhou University Second Hospital
2023
Lemuel Shattuck Hospital
2023
Zhejiang University
2008-2022
First Affiliated Hospital Zhejiang University
2008-2022
Xi'an University of Architecture and Technology
2022
Abstract Here the Human Pangenome Reference Consortium presents a first draft of human pangenome reference. The contains 47 phased, diploid assemblies from cohort genetically diverse individuals 1 . These cover more than 99% expected sequence in each genome and are accurate at structural base pair levels. Based on alignments assemblies, we generate that captures known variants haplotypes reveals new alleles structurally complex loci. We also add 119 million pairs euchromatic polymorphic...
Abstract The recent advances in sequencing technologies enable the assembly of individual genomes to quality reference genome. How integrate multiple from same species and make integrated representation accessible biologists remains an open challenge. Here, we propose a graph-based data model associated formats represent while preserving coordinate linear We implement our ideas minigraph toolkit demonstrate that can efficiently construct pangenome graph compactly encode tens thousands...
Abstract The short arms of the human acrocentric chromosomes 13, 14, 15, 21 and 22 (SAACs) share large homologous regions, including ribosomal DNA repeats extended segmental duplications 1,2 . Although resolution these regions in first complete assembly a genome—the Telomere-to-Telomere Consortium’s CHM13 (T2T-CHM13)—provided model their homology 3 , it remained unclear whether patterns were ancestral or maintained by ongoing recombination exchange. Here we show that contain...
Abstract The Human Pangenome Reference Consortium (HPRC) presents a first draft human pangenome reference. contains 47 phased, diploid assemblies from cohort of genetically diverse individuals. These cover more than 99% the expected sequence and are accurate at structural base-pair levels. Based on alignments assemblies, we generated that captures known variants haplotypes, reveals novel alleles structurally complex loci, adds 119 million base pairs euchromatic polymorphic 1,529 gene...
Abstract Single-nucleotide variants (SNVs) in segmental duplications (SDs) have not been systematically assessed because of the limitations mapping short-read sequencing data 1,2 . Here we constructed 1:1 unambiguous alignments spanning high-identity SDs across 102 human haplotypes and compared pattern SNVs between unique duplicated regions 3,4 We find that are elevated 60% to estimate at least 23% this increase is due interlocus gene conversion (IGC) with up 4.3 megabase pairs SD sequence...
Tertiary lymphoid structures (TLSs) have been proposed to assess the prognosis of patients with cancer. Here, we investigated prognostic value and relevant mechanisms TLSs in colorectal cancer liver metastases (CRCLM).603 CRCLM treated by surgical resection from three centers were included. The categorized according their anatomic subregions quantified, a TLS scoring system was established for intratumor region (T score) peritumor (P score). Differences relapse-free survival (RFS) overall...
Alzheimer's disease (AD) is characterized by the accumulation of β-amyloid peptide (Aβ) and loss neurons. Recently, a growing body evidences have indicated that as herbal compound naturally derived from grapes, resveratrol modulates pathophysiology AD, however, with largely unclear mechanism. Therefore, we aimed to investigate protection against neurotoxicity 25–35 (Aβ25–35) further explore its underlying mechanism in present study. PC12 cells were injuried Aβ25–35, at different...
Objective: Neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte (PLR) have been emerging as the novel inflammatory biomarkers for determining prognosis of various diseases. This study aimed to investigate individual joint effects NLR PLR on functional outcomes acute ischemic stroke (AIS). Methods: Our involved 448 eligible patients with first-ever AIS. Clinical laboratory data were collected admission within 72 h from onset. Unfavorable outcome was defined a modified Rankin Scale...
Background Recent studies have shown that high expression levels of class I histone deacetylases (HDACs) correlate with malignant phenotype and poor prognosis in some human tumors. However, the patterns prognostic role HDAC isoforms hepatocellular carcinoma (HCC) remain unclear. Methodology/Principal Findings The clinical significance were assessed by immunohistochemistry a cohort 43 hepatitis B virus-associated HCC patients treated liver transplantation. In addition, effects inhibition on...
TLR4 (Toll-like receptor 4) and B7-H1, which were known to be restricted immune cells in the past, found aberrantly expressed a majority of tumor cells, facilitating evasion from surveillance. Our study demonstrated that activation signaling bladder cancer up-regulated B7-H1 expression. Furthermore, this regulation was significantly attenuated by ERK or JNK inhibitor. results elucidated molecule mechanism expression through may suggest new strategies down-regulating cancer-associated for treatment.
Curcumin has been shown to have high cytotoxicity towards various cancer cell lines, but its water insolubility and instability make bioavailability exceedingly low and, thus, it is generally inactive in vivo anticancer tests. Here, we report an intracellular-labile amphiphilic surfactant-like curcumin prodrug – conjugated with two short oligo(ethylene glycol) (Curc-OEG) chains via β-thioester bonds that are labile the presence of intracellular glutathione esterase. Curc-OEG formed stable...
Hepatocellular carcinoma (HCC) is a highly aggressive malignancy. Nanosecond pulsed electric field (nsPEF) new technology destroying tumor cells with non-thermal high voltage using ultra-short pulses. The study's aim was to evaluate the ablation efficacy of nsPEFs human HCC cell lines and metastatic potential xenograft model on BALB/c nude mice. in vivo study showed induced death dose dependent manner. On hepatocellular line (HCCLM3) mice model, growth inhibited significantly nsPEF-treated-...
Experimental cholestatic liver fibrosis was performed by bile duct ligation (BDL) in mice, and significant injury observed 15 days. Administration of baicalin mice significantly ameliorates fibrosis. associated with induced gene expression fibrotic markers such as collagen I, fibronectin, alpha smooth muscle actin (SMA), connective tissue growth factor (CTGF); increased inflammatory cytokines (TNF α , MIP1 IL1 β MIP2); oxidative stress reactive oxygen species‐ (ROS‐) inducing enzymes (NOX2...
Only a small fraction of patients with cancer receiving immune checkpoint therapy (ICT) respond, which is associated tumor microenvironment (TIME) subtypes and tumor-infiltrating lymphocytes (TILs).To examine whether germline variants natural killer (NK) cells, key component the system, are TIME subtypes, abundance TILs, response to ICT, clinical outcomes, risk.This genetic association study explored examined genomic information prognosis, risk. Clinical information, RNA sequencing,...
Abstract Background In the metagenomic assembly of a microbial community, abundant species are often thought to assemble well given their deeper sequencing coverage. This conjuncture is rarely tested or evaluated in practice. We do not know how many missing and have an approach recover them. Results Here, we propose k -mer based 16S RNA methods measure completeness metagenome assembly. show that even with PacBio high-fidelity (HiFi) reads, assembled, as high strain diversity may lead...
Abstract Resveratrol (3,5,4'‐tihydroxy‐trans‐stilbene), a polyphenolic phytoalexin found in the skin and seeds of grapes, has been reported to possess wide range biological pharmacological activities including antioxidant, anti‐inflammatory, antimutagenic effects. The present study intended explore neuroprotective effects resveratrol against Aβ 25–35 ‐induced neurotoxicity cultured mouse cortical neurons possible mechanisms involved. For this purpose, were exposed 30 μM absence or presence...
Magneto-structural correlation studies of mononuclear intermediate S = 3/2 Fe(III) complexes, (PMe3)2FeCl3 (1) and (PMe2Ph)2FeCl3 (2), demonstrate the influence local symmetry on magnetic anisotropy. Symmetric compound 1 is characterized by a zero-field splitting (ZFS) parameter D −50(2) cm–1, leading to observation slow relaxation with an energy barrier 81 cm–1 along hysteresis up 4 K, whereas symmetrically perturbed 2 displays much reduced ZFS −17(1) Ueff 46 cm–1.