A5029003002- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Nonlinear Dynamics and Pattern Formation
- Fluid Dynamics and Thin Films
- Fungal and yeast genetics research
- Microfluidic and Bio-sensing Technologies
- Advanced Mathematical Identities
- Advanced Combinatorial Mathematics
- Micro and Nano Robotics
- Theoretical and Computational Physics
- Solidification and crystal growth phenomena
- Mathematical functions and polynomials
- Genomics and Chromatin Dynamics
- Acoustic Wave Resonator Technologies
- Granular flow and fluidized beds
- Neural Networks and Applications
- Chromosomal and Genetic Variations
- nanoparticles nucleation surface interactions
- 3D Printing in Biomedical Research
- Gene Regulatory Network Analysis
- Reproductive Biology and Fertility
- Plant Reproductive Biology
- Particle Dynamics in Fluid Flows
- Pickering emulsions and particle stabilization
- Cardiomyopathy and Myosin Studies
Stowers Institute for Medical Research
2016-2025
University of Chicago
2023
Kansas City Kansas Community College
2021
New York University
2017
Technion – Israel Institute of Technology
1992-2015
Ural Federal University
2014
University of California, Davis
2003-2007
Northwestern University
2001-2003
Applied Mathematics (United States)
2000-2003
Michigan Technological University
2003
The Arp2/3 complex nucleates the formation of dendritic actin network at leading edge motile cells, but it is still unclear if plays a critical role in lamellipodia protrusion and cell motility. Here, we differentiated fibroblast cells from isogenic mouse embryonic stem with or without disruption ARPC3 gene, which encodes p21 subunit complex. ARPC3−/− fibroblasts were unable to extend generated dynamic edges composed primarily filopodia-like protrusions, formin proteins (mDia1 mDia2)...
Abstract The short arms of the human acrocentric chromosomes 13, 14, 15, 21 and 22 (SAACs) share large homologous regions, including ribosomal DNA repeats extended segmental duplications 1,2 . Although resolution these regions in first complete assembly a genome—the Telomere-to-Telomere Consortium’s CHM13 (T2T-CHM13)—provided model their homology 3 , it remained unclear whether patterns were ancestral or maintained by ongoing recombination exchange. Here we show that contain...
Polar body extrusion during oocyte maturation is critically dependent on asymmetric positioning of the meiotic spindle, which established through migration meiosis I (MI) spindle/chromosomes from interior to a subcortical location. In this study, we show that MI chromosome biphasic and driven by consecutive actin-based pushing forces regulated two actin nucleators, Fmn2, formin family protein, Arp2/3 complex. Fmn2 was recruited endoplasmic reticulum structures surrounding where it nucleated...
Neurons have a membrane periodic skeleton (MPS) composed of actin rings interconnected by spectrin. Here, combining chemical and genetic gain- loss-of-function assays, we show that in rat hippocampal neurons the MPS is an actomyosin network controls axonal expansion contraction. Using super-resolution microscopy, analyzed localization non-muscle myosin II (NMII). We active NMII light chains are colocalized with organized circular manner throughout axon shaft. In contrast, heavy mostly...
Actuation powered by a rotating magnetic field is promising method of controlled steering micro(nano)metric synthetic propellers through fluids. Such actuation relies on torque, which product the driving and dipolar moment possessed micro-/nanopropeller nontrivial shape allowing for rotation–translation coupling. While ferromagnetic (permanently magnetized) microbots have been studied extensively, superparamagnetic (susceptible to magnetization, not possessing remanent magnetization) did get...
Abstract Despite its widespread existence, the adaptive role of aneuploidy (the abnormal state having an unequal number different chromosomes) has been a subject debate. Cellular associated with enhanced resistance to stress, whereas on organismal level it is detrimental multicellular species. Certain aneuploid karyotypes are deleterious for specific environments, but karyotype diversity in population potentiates evolution. To reconcile these paradoxical observations, this review...
The ability to break symmetry and polarize through self-organization is a fundamental feature of cellular systems. A prevailing theory in yeast posits that breaking occurs via positive feedback loop, wherein the adaptor protein Bem1 promotes local activation accumulation Cdc42 by directly tethering Cdc42GTP with its guanine nucleotide exchange factor (GEF) Cdc24. In this paper, we find neither nor bind required for cell polarization. Instead, functions primarily boosting GEF activity, role...
We investigate computationally the self-organization and contraction of an initially random actomyosin ring. In framework a detailed physical model for ring cross-linked actin filaments myosin-II clusters, we derive force balance equations solve them numerically. find that to contract, have treadmill be sufficiently cross linked, myosin has processive. The simulations reveal how scales with mechanochemical parameters. For example, they show made longer generates greater but contracts slower....
The kinetochore is a large, evolutionarily conserved protein structure that connects chromosomes with microtubules. During chromosome segregation, outer components track depolymerizing ends of microtubules to facilitate the separation into two cells. In budding yeast, each has point centromere upon which single built, attaches microtubule. This defined architecture facilitates quantitative examination kinetochores during cell cycle. Using three independent measures—calibrated imaging, FRAP,...
Cells employ protrusive leading edges to navigate and promote their migration in diverse physiological environments. Classical models of leading-edge protrusion rely on a treadmilling dendritic actin network that undergoes continuous assembly nucleated by the Arp2/3 complex, forming ruffling lamellipodia. Recent work demonstrated, however, that, absence fibroblast cells adopt edge with filopodia-like protrusions (FLPs) maintain an ability move, albeit altered responses different...