A5086745660- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Retinal Diseases and Treatments
- Fungal and yeast genetics research
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Retinopathy of Prematurity Studies
- Retinal and Macular Surgery
- Advanced biosensing and bioanalysis techniques
- Glaucoma and retinal disorders
- Virus-based gene therapy research
- Plant Reproductive Biology
- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- Fibroblast Growth Factor Research
- Endoplasmic Reticulum Stress and Disease
- Phagocytosis and Immune Regulation
- Plant Surface Properties and Treatments
- Pancreatic function and diabetes
Fraternal Order of Eagles
2025
University of Iowa
2025
Indiana University – Purdue University Indianapolis
2020-2024
Hypertension Institute
2021-2024
Indiana University School of Medicine
2021-2023
Neurosciences Institute
2023
Indiana University
2023
Johns Hopkins University
2016-2020
Johns Hopkins Medicine
2019-2020
National Institutes of Health
2014-2016
Cell-cell contact inhibition and the mechanical environment of cells have both been shown to regulate YAP nuclear localization modulate cell proliferation. Changes in cellular contractility by genetic, pharmacological, matrix stiffness perturbations localization. However, because F-actin organization are interconnected cytoskeletal properties, it remains unclear which these distinctly regulates Here we show that absence cell-cell contact, actomyosin suppresses phosphorylation at Ser(112),...
Abstract Mitochondrial dysfunctions are widely afflicted in central nervous system (CNS) disorders with minimal understanding on how to improve mitochondrial homeostasis promote neuroprotection. Here we have used human stem cell differentiated retinal ganglion cells (hRGCs) of the CNS, which highly sensitive towards due their unique structure and function, identify mechanisms for improving quality control (MQC). We show that hRGCs efficient maintaining through rapid degradation biogenesis...
Retinal ganglion cell (RGC) degeneration is the root cause for vision loss in glaucoma as well other forms of optic neuropathy. A variety studies have implicated abnormal mitochondrial quality control (MQC) contributing to RGC damage and neuropathies. The ability differentiate human pluripotent stem cells (hPSCs) into RGCs provides an opportunity study MQC great detail. Degradation damaged mitochondria a critical step MQC, here we used hPSC-derived (hRGCs) analyze how altered degradation...
Abstract Cationic liposome-mediated delivery of drugs, DNA, or RNA plays a pivotal role in small molecule therapy, gene editing, and immunization. However, our current knowledge regarding the cellular structures that facilitate this process remains limited. Here, we used human pluripotent stem cells (hPSCs), which form compact colonies consisting dynamically active at periphery epithelial-like core. We discovered colony edges selectively got transfected by cationic liposomes through...
ABSTRACT Retinal ganglion cell (RGC) degeneration is the root cause for vision loss in glaucoma as well other forms of optic neuropathies. Genetic analysis indicated abnormal mitochondrial quality control (MQC) a major risk factor However, nothing known on how MQC regulates human retinal (hRGC) health and survival. Human pluripotent stem cells (hPSCs) provide opportunity to differentiate hRGCs understand associated hRGC great detail. Degradation damaged mitochondria very critical step MQC,...
Background:Retinal ganglion cells (RGCs) are highly energy dependent due to their continuous action potential firing requirements and long unmyelinated axons hence susceptible mitochondrial dysfunctions, observed in glaucoma. Dr. Das’s lab recently had identified Tank-binding kinase 1 (TBK1) inhibition by BX795 drug activates biogenesis promotes RGC protection with glaucomatous Optineurin (OPTN-E50K) mutation. OPTN is a critical player for mitophagy. It still not clear if activation of...
Mitochondrial dysfunctions are widely afflicted in central nervous system (CNS) disorders with minimal understanding on how to improve mitochondrial homeostasis promote neuroprotection. Here we used human stem cell differentiated retinal ganglion cells (hRGCs) of the CNS, which highly sensitive towards due their unique structure and function, identify mechanisms for improving quality control (MQC). We found that hRGCs efficient maintaining through rapid degradation biogenesis mitochondria...
Abstract Cationic liposome-mediated delivery of drugs, DNA, or RNA plays a pivotal role in small molecule therapy, gene editing, and immunization. However, our current knowledge regarding the cellular structures that facilitate this process remains limited. Here, we used human pluripotent stem cells (hPSCs), which form compact colonies consisting dynamically active at periphery epithelial-like core. We discovered colony edges selectively got transfected by cationic liposomes through Arp2/3...
CRISPR-Cas9 mediated genome editing of human pluripotent stem cells (hPSCs) provides strong avenues for disease modeling, drug discovery and cell replacement therapy. Genome hPSCs is an extremely inefficient process requires complex gene delivery selection methods to improve edit efficiency which are not ideal clinical applications. Here, we have shown a free simple lipofectamine based transfection method where single plasmid encoding guide RNA (gRNA) Cas9 selectively transfected at the...