A5067830488- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Fungal and yeast genetics research
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- DNA Repair Mechanisms
- Genetic Neurodegenerative Diseases
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Protein Structure and Dynamics
- Prion Diseases and Protein Misfolding
- Chromosomal and Genetic Variations
- Enzyme Structure and Function
- Heat shock proteins research
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Microbial Metabolic Engineering and Bioproduction
Stowers Institute for Medical Research
2015-2024
North Carolina State University
2023
University of Kansas Medical Center
2023
The Open University
2023
University of Cambridge
2023
University of Alabama at Birmingham
2023
University of Kansas
2003-2009
Baylor University
2009
University of California, Irvine
2007-2008
Fluor (United States)
2008
The Arp2/3 complex nucleates the formation of dendritic actin network at leading edge motile cells, but it is still unclear if plays a critical role in lamellipodia protrusion and cell motility. Here, we differentiated fibroblast cells from isogenic mouse embryonic stem with or without disruption ARPC3 gene, which encodes p21 subunit complex. ARPC3−/− fibroblasts were unable to extend generated dynamic edges composed primarily filopodia-like protrusions, formin proteins (mDia1 mDia2)...
Nuclear pore complexes (NPCs) mediate the nucleocytoplasmic transport of macromolecules. Here we provide a structure isolated yeast NPC in which inner ring is resolved by cryo-EM at sub-nanometer resolution to show how flexible connectors tie together different structural and functional layers. These may be targets for phosphorylation regulated disassembly cells with an open mitosis. Moreover, some nucleoporin pairs factors have similar interaction motifs, suggests evolutionary mechanistic...
A rapid, homogeneous aptamer-based bioanalysis is reported for the sensitive detection of immunoglobulin E (IgE) using fluorescence polarization (FP). 5‘-End-labeled D17.4 DNA aptamer was used IgE based on anisotropy differences labeled ligand. Two different fluorophores, fluorescein and Texas Red, were to analyze in low-nanomolar range with high specificity. Measurable changes observed a short equilibration time. Analysis binding data reveals possible cooperative process solution. The...
Ribosomal DNA is one of the most variable regions in human genome with respect to copy number. Despite importance rDNA for cellular function, we know virtually nothing about what governs its number, stability, and sequence mammalian due challenges associated mapping analysis. We applied computational droplet digital PCR approaches measure number normal cancer states mouse genomes. find that can change Counterintuitively, genomes show a loss copies, accompanied by global co-variation. The...
Significance Because inaccurate chromosome segregation during meiosis is a leading cause of miscarriage in humans, we seek to understand how homologous chromosomes segregate properly. Meiotic occurs with fidelity only the presence synaptonemal complex (SC), protein structure that assembles between homologs and facilitates occurrence crossing over. Although some functions SC are evolutionarily conserved, mechanisms underlying its multiple roles meiosis, as well organizational variances among...
Regulation of hematopoietic stem cells (HSCs) by bone marrow (BM) niches has been extensively studied; however, whether and how HSC subpopulations are distinctively regulated BM remain unclear. Here, we functionally distinguished reserve HSCs (rHSCs) from primed (pHSCs) based on their response to chemotherapy examined they dichotomously niches. Both pHSCs rHSCs supported long-term hematopoiesis in homeostasis; were sensitive but resistant chemotherapy. Surviving restored the pool...
Polar body extrusion during oocyte maturation is critically dependent on asymmetric positioning of the meiotic spindle, which established through migration meiosis I (MI) spindle/chromosomes from interior to a subcortical location. In this study, we show that MI chromosome biphasic and driven by consecutive actin-based pushing forces regulated two actin nucleators, Fmn2, formin family protein, Arp2/3 complex. Fmn2 was recruited endoplasmic reticulum structures surrounding where it nucleated...
Understanding the protein composition of inner nuclear membrane (INM) is fundamental to elucidating its role in normal function and disease; however, few tools exist examine INM living cells, INM-specific proteome remains poorly characterized. Here, we adapted split green fluorescent (split-GFP) systematically localize known predicted integral proteins Saccharomyces cerevisiae as opposed outer membrane. Our data suggest that components endoplasmic reticulum (ER) well other organelles are...
Amyloids are ordered protein aggregates that typically associated with neurodegenerative diseases and cognitive impairment. By contrast, the amyloid-like state of neuronal RNA binding Orb2 in Drosophila was recently implicated memory consolidation, but it remains unclear what features this functional give rise to such diametrically opposed behaviour. Here, using an array biophysical, cell biological behavioural assays we have characterized structural from monomer amyloid state. Surprisingly,...
Ribosomal RNA (rRNA) transcription by polymerase I (Pol I) is a critical rate-limiting step in ribosome biogenesis, which essential for cell survival. Despite its global function, disruptions biogenesis cause tissue-specific birth defects called ribosomopathies, frequently affect craniofacial development. Here, we describe cellular and molecular mechanism underlying the susceptibility of development to Pol transcription. We show that subunits are highly expressed neuroepithelium neural crest...
Ribosome biogenesis is a vital and highly energy-consuming cellular function occurring primarily in the nucleolus. Cancer cells have an elevated demand for ribosomes to sustain continuous proliferation. This study evaluated impact of existing anticancer drugs on nucleolus by screening library compounds that induce nucleolar stress. For readout, novel parameter termed 'nucleolar normality score' was developed measures ratio fibrillar center granular component proteins nucleoplasm. Multiple...
Although Lepidopteran females build a synaptonemal complex (SC) in pachytene, homologs do not crossover, necessitating an alternative method of homolog conjunction. In Bombyx mori oocytes, the SC breaks down at end and associations are maintained by large oocyte-specific structure, which we call bivalent bridge (BB), connecting paired homologs. The BB is derived from least some components lateral elements (LEs). It contains HORMAD protein HOP1 LE SYCP2 formed fusion two derivatives. As...
The centromere is a specialized chromosomal structure that regulates chromosome segregation. Centromeres are marked by histone H3 variant. In budding yeast, the variant Cse4 present in single centromeric nucleosome. Experimental evidence supports several different models for of nucleosomes. To investigate copy number live we developed method coupling fluorescence correlation spectroscopy and calibrated imaging. We find nucleosomes have one during most cell cycle, whereas two copies detected...
Cohesin is a protein complex known for its essential role in chromosome segregation. However, cohesin and associated factors have additional functions transcription, DNA damage repair, condensation. The human cohesinopathy diseases are thought to stem not from defects segregation but gene expression. of expression well understood. We used budding yeast strains bearing mutations analogous the disease alleles under control their native promoter study These do significantly affect...