A5084856496- RNA Research and Splicing
- Nuclear Structure and Function
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Genetic Neurodegenerative Diseases
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- vaccines and immunoinformatics approaches
- Radioactive element chemistry and processing
- Fungal and yeast genetics research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Geotechnical Engineering and Underground Structures
- Mosquito-borne diseases and control
- Cancer-related gene regulation
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Health and Lifestyle Studies
- Ubiquitin and proteasome pathways
Rockefeller University
2012-2024
St. John's University
2021-2024
St. John's School
2021
University of Colorado Boulder
2015
King County Department of Natural Resources and Parks
2012
University of Washington
2003
University Hospitals of Cleveland
1999-2001
Case Western Reserve University
1999-2001
Center for Human Genetics
1999
Cajal bodies (CBs) are nuclear suborganelles involved in the biogenesis of small ribonucleoproteins (snRNPs). In addition to snRNPs, they highly enriched basal transcription and cell cycle factors, nucleolar proteins fibrillarin (Fb) Nopp140 (Nopp), survival motor neuron (SMN) protein complex, CB marker protein, p80 coilin. We report generation knockout mice lacking COOH-terminal 487 amino acids Northern Western blot analyses demonstrate that we have successfully removed full-length coilin...
Protein lysine methylation is one of the most widespread post-translational modifications in nuclei eukaryotic cells. Methylated lysines on histones and nonhistone proteins promote formation protein complexes that control gene expression DNA replication repair. In cytoplasm, however, role complex not well established. Here we report cytoplasmic chaperone Hsp90 methylated by methyltransferase Smyd2 various cell types. muscle, contributes to a containing Smyd2, Hsp90, sarcomeric titin....
The emergence of SARS-CoV-2 variants threatens current vaccines and therapeutic antibodies urgently demands powerful new therapeutics that can resist viral escape. We therefore generated a large nanobody repertoire to saturate the distinct highly conserved available epitope space spike, including S1 receptor binding domain, N-terminal S2 subunit, identify sites may reflect novel mechanisms neutralization. Structural mapping functional assays show indeed these stable monovalent nanobodies...
Coiled bodies (CBs) are nuclear organelles involved in the metabolism of small RNAs (snRNAs) and histone messages. Their structural morphology molecular composition have been conserved from plants to animals. CBs preferentially specifically associate with genes that encode U1, U2, U3 snRNAs as well cell cycle–regulated loci. A common link among these previously identified CB-associated is they either clustered or tandemly repeated human genome. In an effort identify additional loci CBs, we...
Picornaviruses are small RNA viruses that hijack host cell machinery to promote their replication. During infection, these express two proteases, 2Apro and 3Cpro, which process viral proteins. They also subvert a number of functions, including innate immune responses, protein synthesis, intracellular transport, by utilizing poorly understood mechanisms for rapidly specifically targeting critical Here, we used proteomic tools characterize interacting partners, mechanisms. Our data indicate...
U small nuclear RNAs (snRNAs) and mRNAs are both transcribed by RNA polymerase II (Pol II), but the snRNAs have unusual TATA-less promoters neither spliced nor polyadenylated; instead, 3′ processing is directed a highly conserved end formation signal that requires initiation from an snRNA promoter. Here we show C-terminal domain (CTD) of Pol required for efficient U2 transcription, as it mRNA transcription. However, CTD kinase inhibitors, such 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole...
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SUMMARY Despite the great promise of vaccines, COVID-19 pandemic is ongoing and future serious outbreaks are highly likely, so that multi-pronged containment strategies will be required for many years. Nanobodies smallest naturally occurring single domain antigen binding proteins identified to date, possessing numerous properties advantageous their production use. We present a large repertoire high affinity nanobodies against SARS-CoV-2 Spike protein with excellent kinetic viral...
King County and the City of Bellevue (WA) each routinely deal with construction new replacement sewers in their respective systems. The "Bellevue Influent Trunk West CBD Sewer Replacement Project" represents a collaboration two agencies to replace existing trunk lines that serve Bellevue's Central Business District pass through high-density residential neighborhood. pipelines were badly deteriorated reaching capacity, potential for overflows nearby creek. This project was undertaken meet...