A5100344259- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Lung Cancer Research Studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Head and Neck Cancer Studies
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- HER2/EGFR in Cancer Research
- Lung Cancer Treatments and Mutations
- Advanced Biosensing Techniques and Applications
- T-cell and B-cell Immunology
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Aquaculture disease management and microbiota
- Corneal Surgery and Treatments
- Gene expression and cancer classification
- Circular RNAs in diseases
Shanghai Jiao Tong University
2016-2025
Shandong Eye Hospital
2022-2025
Shandong First Medical University
2019-2025
Foshan University
2017-2025
Fujian Medical University
2020-2025
Xingtai People's Hospital
2018-2025
Women's Hospital, School of Medicine, Zhejiang University
2024-2025
Fuzhou Maternity and Child Health Care Hospital
2025
Hebei Medical University
2018-2025
Stowers Institute for Medical Research
2015-2024
Genomic findings underscore the heterogeneity of head and neck squamous cell carcinoma (HNSCC). Identification mutations that predict therapeutic response would be a major advance. We determined mutationally altered, targetable mitogenic pathways in large HNSCC cohort. Analysis whole-exome sequencing data from 151 tumors revealed phosphoinositide 3-kinase (PI3K) pathway to most frequently mutated oncogenic (30.5%). PI3K pathway-mutated harbored significantly higher rate known cancer genes....
MicroRNAs (miRNAs) regulate gene expression. It has been suggested that obtaining miRNA expression profiles can improve classification, diagnostic, and prognostic information in oncology. Here, we sought to comprehensively identify the miRNAs are overexpressed lung cancer by conducting microarray profiling on normal versus adjacent cancers from transgenic mice. We found miR-136, miR-376a, miR-31 were each prominently murine cancers. Real-time RT-PCR situ hybridization (ISH) assays confirmed...
Abstract We still know very little about how the human immune system responds to SARS-CoV-2. Here we construct a SARS-CoV-2 proteome microarray containing 18 out of 28 predicted proteins and apply it characterization IgG IgM antibodies responses in sera from 29 convalescent patients. find that all these patients had specifically bind proteins, particularly N protein S1 protein. Besides significant antibody ORF9b NSP5 are also identified. show specific signal positively correlates with age...
Response to trastuzumab in metastatic breast cancer correlates with expression of the high binding variant (158V) activating Fcγ receptor IIIA (CD16A). We engineered MGAH22, a chimeric anti-HER2 monoclonal antibody specificity and affinity similar trastuzumab, an Fc domain for increased both alleles human CD16A. MGAH22 was compared identical mAb except wild type domain. Antibody-dependent cell cytotoxicity (ADCC) assays were performed HER2-expressing cells as targets PBMC or purified NK...
Tebotelimab, a bispecific PD-1×LAG-3 DART molecule that blocks both PD-1 and LAG-3, was investigated for clinical safety activity in phase 1 dose-escalation cohort-expansion trial patients with solid tumors or hematologic malignancies disease progression on previous treatment. Primary endpoints were maximum tolerated dose of tebotelimab when administered as single agent (n = 269) combination the anti-HER2 antibody margetuximab 84). Secondary included anti-tumor activity. In advanced cancer...
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) infection, is currently the deadliest infectious disease in human that can evolve to severe forms. A comprehensive immune landscape for Mtb infection critical achieving TB cure, especially patients. We performed single-cell RNA transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing of 213,358 cells from 27 samples, including 6 healthy donors 21 active patients with varying severity (6 mild, moderate 9 cases). Two published...
Abstract Monoclonal antibodies (mAb) are widely used in the treatment of non-Hodgkin's lymphoma and autoimmune diseases. Although mechanism action vivo is not always known, therapeutic activity several approved mAbs depends on binding Fcγ regions to low-affinity receptors (FcγR) expressed effector cells. We did functional genetic screens identify IgG1 Fc domains with improved activating receptor CD16A (FcγRIIIA) reduced inhibitory receptor, CD32B (FcγRIIB). Identification new amino acid...
NODAL and its signaling pathway are known to play a key role in specification patterning of vertebrate embryos. Mutations several genes encoding components the have previously been implicated pathogenesis human left–right (LR) defects. Therefore, NODAL, member TGF-β superfamily developmental regulators, is strong candidate be functionally involved congenital LR axis defects or heterotaxy. Here we investigated whether variants present patients with heterotaxy and/or isolated cardiovascular...
Summary Human CD32B (FcγRIIB), the low‐affinity inhibitory Fcγ receptor (FcγR), is highly homologous in its extracellular domain to CD32A (FcγRIIA), an activating FcγR. Available monoclonal antibodies (mAb) against region of recognize both receptors. Through immunization mice transgenic for human CD32A, we generated a set specific with no cross‐reactivity as determined by enzyme‐linked immunosorbent assay and surface plasmon resonance recombinant CD32B, fluorescence‐activated cell sorting...
Radiotherapy is used in locally advanced pancreatic cancers which it can improve survival combination with gemcitabine. However, prognosis still poor this setting more effective therapies remain needed. MLN4924 an investigational small molecule currently phase I clinical trials. inhibits NAE (NEDD8 Activating Enzyme), a pivotal regulator of the E3 ubiquitin ligase SCF (SKP1, Cullins, and F-box protein), that has been implicated recently DNA damage repair. In study, we provide evidence be as...
Ribosomal DNA is one of the most variable regions in human genome with respect to copy number. Despite importance rDNA for cellular function, we know virtually nothing about what governs its number, stability, and sequence mammalian due challenges associated mapping analysis. We applied computational droplet digital PCR approaches measure number normal cancer states mouse genomes. find that can change Counterintuitively, genomes show a loss copies, accompanied by global co-variation. The...
Our previous study indicated that GPC3-targeted chimeric antigen receptor (CAR) T cell therapy has a high safety profile in patients with hepatocellular carcinoma (HCC). However, the response rate requires further improvement. Here, we analyzed combined effect of GPC3-CAR cells and sorafenib both immunocompetent immunodeficient mouse models carcinoma. In model, CAR (mCAR) induced regression small tumors (approximately 130 mm3 tumor volume) but had no on large, established 400 volume)....
EZH2, the catalytic component of polycomb repressive complex 2 (PRC2), is frequently overexpressed in human cancers and contributes to tumor initiation progression, part through transcriptional silencing suppressor genes. A number noncoding RNAs (ncRNAs) recruit EZH2 specific chromatin loci, where they modulate gene expression. Here, we used RNA immunoprecipitation sequencing (RIP-seq) profile EZH2-associated transcripts gastric cancer cell lines. We identified 8,256 transcripts, including...