A5100652718- Genomics, phytochemicals, and oxidative stress
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Pharmacological Effects of Medicinal Plants
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Radiopharmaceutical Chemistry and Applications
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Circular RNAs in diseases
- Bone Metabolism and Diseases
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Glutathione Transferases and Polymorphisms
- Tissue Engineering and Regenerative Medicine
- Bioactive Compounds and Antitumor Agents
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Drug Transport and Resistance Mechanisms
- CRISPR and Genetic Engineering
- Infant Nutrition and Health
- Cancer Mechanisms and Therapy
Shenzhen Children's Hospital
2024-2025
Southern Medical University
2015-2024
Ministry of Agriculture and Rural Affairs
2024
Nanjing Agricultural University
2015-2024
Chinese Academy of Sciences
1999-2024
Guangdong Provincial People's Hospital
2024
China Tobacco
2024
Institute of Psychology, Chinese Academy of Sciences
2024
University of Chinese Academy of Sciences
2024
Wenzhou Medical University
2016-2024
Genomic findings underscore the heterogeneity of head and neck squamous cell carcinoma (HNSCC). Identification mutations that predict therapeutic response would be a major advance. We determined mutationally altered, targetable mitogenic pathways in large HNSCC cohort. Analysis whole-exome sequencing data from 151 tumors revealed phosphoinositide 3-kinase (PI3K) pathway to most frequently mutated oncogenic (30.5%). PI3K pathway-mutated harbored significantly higher rate known cancer genes....
We have shown previously that sulforaphane (SFN), a constituent of many edible cruciferous vegetables including broccoli, suppresses growth prostate cancer cells in culture as well vivo by causing apoptosis, but the sequence events leading to cell death is poorly defined. Using PC-3 and DU145 human model, we now demonstrate, for first time, initial signal SFN-induced apoptosis derived from reactive oxygen species (ROS). Exposure growth-suppressive concentrations SFN resulted ROS generation,...
Frequent gene amplification of the receptor-activated calcium-dependent chloride channel TMEM16A (TAOS2 or ANO1) has been reported in several malignancies. However, its involvement human tumorigenesis not previously studied. Here, we show a functional role for tumor growth. We found overexpression 80% head and neck squamous cell carcinoma (SCCHN), which correlated with decreased overall survival patients SCCHN. significantly promoted anchorage-independent growth vitro, loss resulted...
The present study shows that oral gavage of 6 mumol d,l-sulforaphane (SFN), a synthetic analogue cruciferous vegetable-derived L isomer, thrice per week beginning at weeks age, significantly inhibits prostate carcinogenesis and pulmonary metastasis in TRAMP mice without causing any side effects. incidence the prostatic intraepithelial neoplasia well-differentiated (WD) carcinoma were approximately 23% to 28% lower (P < 0.05 compared with control by Mann-Whitney test) dorsolateral (DLP)...
Phenethyl isothiocyanate (PEITC), a constituent of edible cruciferous vegetables such as watercress, not only affords significant protection against chemically induced cancer in experimental rodents but also inhibits growth human cells by causing apoptotic and autophagic cell death. However, the underlying mechanism PEITC-induced death is fully understood. Using LNCaP PC-3 prostate model, we demonstrate that initiated production reactive oxygen species (ROS) resulting from inhibition...
Abstract Epidemiologic studies have revealed an inverse correlation between dietary intake of cruciferous vegetables and the risk breast cancer. We now show that vegetable constituent benzyl isothiocyanate (BITC) effectively suppresses growth cultured human cancer cells (MDA-MB-231 MCF-7) by causing G2-M phase cell cycle arrest apoptosis induction. On other hand, a normal mammary epithelial line (MCF-10A) is significantly more resistant to BITC compared with cells. The BITC-mediated was...
Benzyl isothiocyanate (BITC), a dietary cancer chemopreventive agent, causes apoptosis in MDA-MB-231 and MCF-7 human breast cells, but the mechanism of cell death is not fully understood. We now demonstrate that BITC-induced cells initiated by reactive oxygen species (ROS) due to inhibition complex III mitochondrial respiratory chain. The ROS production were significantly inhibited overexpression catalase Cu,Zn-superoxide dismutase pharmacological DNA-deficient Rho-0 variant was nearly...
Phenethyl isothiocyanate (PEITC) is a promising cancer chemopreventive agent but the mechanism of its anticancer effect not fully understood. We now show, for first time, that PEITC treatment triggers Atg5-dependent autophagic and apoptotic cell death in human prostate cells. Exposure PC-3 (androgen independent, p53 null) LNCaP responsive, wild-type p53) cells to resulted several specific features characteristic autophagy, including appearance membranous vacuoles, formation acidic vesicular...
Abstract Previous studies, including those from our laboratory, have revealed that phenethyl isothiocyanate (PEITC), a constituent of many edible cruciferous vegetables, not only affords significant protection against chemically induced cancer in animal models but also inhibits growth cells culture and vivo by causing cell cycle arrest apoptosis induction. We now report novel response to PEITC involving inhibition angiogenesis vitro ex at pharmacologically achievable concentrations. The...
The loss of microRNA-122 (miR-122) expression is strongly associated with increased invasion and metastasis, poor prognosis hepatocellular carcinoma (HCC), however, the underlying mechanisms remain poorly understood. In present study, we observed that miR-122 over-expression in HCC cell lines Sk-hep-1 Bel-7402 triggered mesenchymal-epithelial transition (MET), as demonstrated by epithelial-like morphological changes, up-regulated epithelial proteins (E-cadherin, ZO-1, α-catenin, occludin,...
Herpes simplex virus type 1 strain 129 (H129) has represented a promising anterograde neuronal circuit tracing tool, which complements the existing retrograde tracers. However, current H129 derived tracers are multisynaptic, neither bright enough to label details of neurons nor capable determining direct projection targets as monosynaptic tracer.Based on bacterial artificial chromosome H129, we have generated serial recombinant viruses for tracing. Among them, H129-G4 was obtained by...
miR-155 plays critical roles in numerous physiological and pathological processes, however, its function the regulation of blood glucose homeostasis insulin sensitivity underlying mechanisms remain unknown. Here, we reveal that levels are downregulated serum from type 2 diabetes (T2D) patients, suggesting might be involved control diabetes. Gain-of-function loss-of-function studies mice demonstrate has no effects on pancreatic β-cell proliferation function. Global transgenic overexpression...
D,L-Sulforaphane (SFN), a synthetic analogue of cruciferous vegetable-derived isomer l-SFN, suppresses proliferation cancer cells by causing apoptosis but the mechanism cell death is not fully understood. We used LNCaP (wild-type p53) and PC-3 (p53 deficient) human prostate to gain further insights into SFN-induced apoptosis. The line was relatively more sensitive compared with PC-3. SFN treatment caused stabilization p53 protein in cells, SFN-mediated attenuated knockdown protein. Instead,...
The present study was undertaken to gain insights into the molecular mechanism of apoptosis induction by phenethyl isothiocyanate (PEITC), which is a cancer chemopreventive constituent cruciferous vegetables, using PC-3 human prostate cells as model. PEITC-induced cell death in associated with disruption mitochondrial membrane potential, release apoptogenic molecules (cytochrome c and Smac/DIABLO) from mitochondria cytosol generation reactive oxygen species (ROS), were blocked presence...
Abstract Guggulsterone, a constituent of Indian Ayurvedic medicinal plant Commiphora mukul, causes apoptosis in cancer cells but the sequence events leading to cell death is poorly understood. We now show that guggulsterone-induced human prostate caused by reactive oxygen intermediate (ROI)–dependent activation c-Jun NH2-terminal kinase (JNK). Exposure PC-3 and LNCaP inducing concentrations guggulsterone resulted JNK p38 mitogen-activated protein (p38 MAPK) both lines extracellular...
Abstract Our previous studies have shown that z-guggulsterone, a constituent of Indian Ayurvedic medicinal plant Commiphora mukul, inhibits the growth human prostate cancer cells by causing apoptosis. We now report novel response to z-guggulsterone involving inhibition angiogenesis in vitro and vivo. The treatment inhibited capillary-like tube formation (in neovascularization) umbilical vein endothelial (HUVEC) migration HUVEC DU145 concentration- time-dependent manner. z- E-isomers...
Tumor metastasis is the leading cause of death in patients with cancer. However, mechanisms that underlie metastatic progression remain unclear. We examined TMEM16A (ANO1) expression as a key factor shifting tumors between growth and metastasis.We evaluated 26 pairs primary lymph node (LN) tissue from squamous cell carcinoma head neck (SCCHN) for differential TMEM16A. In addition, we identified by which influences tumor motility via proteomic screens lines vivo mouse studies...
The miR-17-92 cluster and its 6 different mature microRNAs, including miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1, miR-92a, play important roles in embryo development, immune system, kidney heart adipose differentiation, aging, tumorigenicity. Currently, increasing evidence indicates that some members of may be critical players spermatogenesis, miR-20a. However, the underlying mechanisms spermatogenesis remain largely unknown. Our results showed targeted disruption testes adult mice...
Phenethyl isothiocyanate (PEITC), a constituent of many cruciferous vegetables, offers significant protection against cancer in animals induced by variety carcinogens. The present study demonstrates that PEITC suppresses proliferation PC-3 cells dose-dependent manner causing G(2)-M-phase cell cycle arrest and apoptosis. Interestingly, phenyl (PITC), which is structural analogue but lacks the -CH(2) spacers link aromatic ring to -N=C=S group, neither inhibited viability nor caused or These...
Abstract The present study was undertaken to gain insights into the molecular mechanism of cell death (apoptosis) by guggulsterone, a constituent Ayurvedic medicinal plant Commiphora mukul, using PC-3 human prostate cancer cells as model. viability cells, but not normal epithelial line (PrEC), reduced significantly on treatment with guggulsterone in concentration-dependent manner. Guggulsterone-mediated suppression proliferation due perturbation cycle progression caused apoptosis induction...
Abstract Benzyl isothiocyanate (BITC), a constituent of cruciferous vegetables such as garden cress, inhibits growth human breast cancer cell lines in culture. The present study was undertaken to determine vivo efficacy BITC against MDA‐MB‐231 xenografts. administration retarded cells subcutaneously implanted female nude mice without causing weight loss or any other side effects. BITC‐mediated suppression xenograft correlated with reduced proliferation revealed by immunohistochemical...