A5101896952- Genomics, phytochemicals, and oxidative stress
- DNA and Nucleic Acid Chemistry
- Reproductive System and Pregnancy
- DNA Repair Mechanisms
- interferon and immune responses
- Bacterial Genetics and Biotechnology
- Inflammasome and immune disorders
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- Bacteriophages and microbial interactions
- Nitrogen and Sulfur Effects on Brassica
- RNA regulation and disease
- Neurological Disease Mechanisms and Treatments
- Biochemical and Molecular Research
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- Cancer therapeutics and mechanisms
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Synthesis and Biological Evaluation
- Traumatic Brain Injury and Neurovascular Disturbances
- T-cell and Retrovirus Studies
- Metabolism and Genetic Disorders
- Neurogenesis and neuroplasticity mechanisms
- Drug-Induced Hepatotoxicity and Protection
Changsha Central Hospital
2024-2025
University of South China
2024-2025
Southern Medical University
2021
Shaanxi Normal University
2020
Harbin Medical University
2014
Second Affiliated Hospital of Harbin Medical University
2014
UPMC Hillman Cancer Center
2005-2007
Hearst (United States)
1995
Cornell University
1993-1995
We have shown previously that sulforaphane (SFN), a constituent of many edible cruciferous vegetables including broccoli, suppresses growth prostate cancer cells in culture as well vivo by causing apoptosis, but the sequence events leading to cell death is poorly defined. Using PC-3 and DU145 human model, we now demonstrate, for first time, initial signal SFN-induced apoptosis derived from reactive oxygen species (ROS). Exposure growth-suppressive concentrations SFN resulted ROS generation,...
D,L-Sulforaphane (SFN), a synthetic analogue of cruciferous vegetable-derived isomer l-SFN, suppresses proliferation cancer cells by causing apoptosis but the mechanism cell death is not fully understood. We used LNCaP (wild-type p53) and PC-3 (p53 deficient) human prostate to gain further insights into SFN-induced apoptosis. The line was relatively more sensitive compared with PC-3. SFN treatment caused stabilization p53 protein in cells, SFN-mediated attenuated knockdown protein. Instead,...
The present study was undertaken to gain insights into the molecular mechanism of apoptosis induction by phenethyl isothiocyanate (PEITC), which is a cancer chemopreventive constituent cruciferous vegetables, using PC-3 human prostate cells as model. PEITC-induced cell death in associated with disruption mitochondrial membrane potential, release apoptogenic molecules (cytochrome c and Smac/DIABLO) from mitochondria cytosol generation reactive oxygen species (ROS), were blocked presence...
Abstract Previous studies have indicated that d,l-sulforaphane (SFN), a synthetic cancer chemopreventive analogue of cruciferous vegetable-derived isomer (−)-1-isothiocyanato-(4R)-(methylsulfinyl)-butane, activates checkpoint kinase 2 (Chk2)–dependent G2-M phase cell cycle arrest in p53-deficient human prostate cells. Because p53 is downstream target Chk2 and known to regulate transition by transcriptional regulation cyclin-dependent (Cdk) inhibitor p21Cip1/Waf1 (p21), the present study was...
The gamma complex (gamma delta delta' chi psi) subassembly of the Escherichia coli replicase initiates processive replication upon assembling ring-shaped beta subunit around DNA. ring acts as a clamp to hold down DNA for highly synthesis. In this report we characterize and psi subunits complex. Both are monomeric, they associate form 1:1 does not with gamma, but binds tightly thereby acting bridge assimilate into structure. stimulated ATPase activities gamma. only when was also present thus...
The γ complex (γδδ′χψ) and τ (τδδ′χψ) clamp loaders require ATP hydrolysis to load β sliding clamps onto DNA. tethers the polymerase (Pol) III∗ replicase DNA for processive synthesis. Pol contains both τ, but only one each of δ, δ′, χ, ψ subunits. Hence, there is ambiguity with respect which loader, or complex, exists in structure. In this study, ATP-binding site mutants have been prepared, these mutants, when assembled into either are inactive loading. These used as a tool determine...
Background and Objective. Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Streptococcus Haemophilus influenzae, Staphylococcus aureus, Pseudomonas aeruginosa, Mycobacterium tuberculosis are primary respiratory bacterial pathogens contributing to morbidity mortality in developing countries. This study evaluated the diagnostic performance of multiplex real-time PCR with fluorescence melting curve analysis (MCA) assay, which was used detect eight simultaneously.A total 157...
We have shown previously that wild-type p53 renders H460 human lung cancer cells more sensitive to apoptosis induction by environmental carcinogen benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), but the mechanism of cell death is not fully understood. The present study provides insights into which BPDE causes in cells. Exposure resulted a concentration-dependent apoptotic characterized cleavage poly(ADP-ribose)polymerase, DNA condensation, and histone-associated fragments released cytosol....
The present study aimed to explore the expression and molecular mechanisms of lncRNA SIX3OS1 in post-stroke cognitive impairment (PSCI). Middle cerebral artery occlusion (MCAO) oxygen-glucose deprivation/reoxygenation (OGD/R) were applied establish an vitro vivo model PSCI. RT-qPCR was conducted examine mRNA levels SIX3OS1, miR-511-3p, RBP4. Morris water maze test used evaluate spatial learning memory ability. Cell viability apoptosis examined by CCK-8 flow cytometry. secretion level...
Various solutions are utilized widely for the isolation, harvesting, sorting, testing and transplantation of neural stem cells (NSCs), whereas effects harvesting media on biological characteristics repair potential NSCs remain unclear. To examine some these effects, were isolated from cortex E14.5 mice exposed to conventional [0.9% saline (Saline), phosphate-buffered (PBS) or artificial cerebrospinal fluid (ACSF)] proliferation culture medium (PCM) different durations at 4°C. Treated grafted...
Objective To investigate the effect of transcription factor E2F1 on proliferation and differentiation adipose mesenchymal stem cells (ADSCs) its underlying mechanism. Methods The primary culture ADSCs were isolated from WT or E2F1-/-mice (6-8 weeks) tissues. After 3rd generation (P4), cell morphologies observed surface markers identified by flow cytometry. The compared between two groups counting kit-8 (CCK-8) Oil-Red O staining assay. expression levels peroxisome proliferator...