A5100397656- Immune cells in cancer
- Epigenetics and DNA Methylation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Bone Tissue Engineering Materials
- Phagocytosis and Immune Regulation
- Nanoplatforms for cancer theranostics
- Reproductive System and Pregnancy
- Ferroptosis and cancer prognosis
- Cancer Immunotherapy and Biomarkers
- Inflammation biomarkers and pathways
- Fibroblast Growth Factor Research
- interferon and immune responses
- Protease and Inhibitor Mechanisms
- Extracellular vesicles in disease
- Inflammatory Biomarkers in Disease Prognosis
- S100 Proteins and Annexins
- Protein Tyrosine Phosphatases
- Mosquito-borne diseases and control
- Multiple Myeloma Research and Treatments
- Adipose Tissue and Metabolism
Sun Yat-sen University
2016-2025
Third Affiliated Hospital of Sun Yat-sen University
2016-2025
Stomatology Hospital
2021-2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
Suzhou Research Institute
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2024
Sichuan University
2019-2023
State Key Laboratory of Biotherapy
2019-2023
Shaanxi University of Chinese Medicine
2023
The peritoneal cavity (PerC) is a unique compartment within which variety of immune cells reside, and from macrophages (MØ) are commonly drawn for functional studies. Here we define two MØ subsets that coexist in PerC adult mice. One, provisionally called the large (LPM), contains approximately 90% unstimulated animals but disappears rapidly following lipopolysaccharide (LPS) or thioglycolate stimulation. These express high levels canonical surface markers, CD11b F4/80. second subset,...
Abstract Emerging evidence indicate that cancer-associated fibroblasts (CAFs) affect tumor progression by reshaping the microenvironment. Neutrophils are prominent components of solid tumors and important in cancer progression. Whether phenotype function neutrophils hepatocellular carcinoma (HCC) influenced CAFs is not well understood. Herein, we investigated effect HCC-derived (HCC-CAFs) on explored biological role this effect. We found HCC-CAFs induced chemotaxis protected them from...
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) infection, is currently the deadliest infectious disease in human that can evolve to severe forms. A comprehensive immune landscape for Mtb infection critical achieving TB cure, especially patients. We performed single-cell RNA transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing of 213,358 cells from 27 samples, including 6 healthy donors 21 active patients with varying severity (6 mild, moderate 9 cases). Two published...
Purpose: Elevated levels of neutrophils have been associated with poor survival in various cancers, but direct evidence supporting a role for the immunopathogenesis human cancers is lacking.Experimental Design: A total 573 patients gastric cancer were enrolled this study. Immunohistochemistry and real-time PCR performed to analyze distribution clinical relevance different microanatomic regions. The regulation function assessed both vitro vivoResults: Increased neutrophil counts peripheral...
Modulation of tumor-associated macrophages (TAMs) holds promise for cancer treatment, mainly relying on M1 signaling activation and pro-inflammatory promotion. Nevertheless, the antitumor activity is often limited by anti-inflammatory factors in tumor microenvironment. Moreover, metabolic function TAMs also critical to progression. However, there are a few strategies that can simultaneously regulate both inflammatory functions achieve safe potent TAMs. Herein, we demonstrate an iron-based...
Abstract Tumor-associated macrophages (TAM) play pivotal roles in tumor progression and metastasis, but the contribution regulation of different macrophage populations remain unclear. Here we show that Notch signaling plays distinct regulating TAM subsets hepatocellular carcinoma (HCC). Myeloid-specific NOTCH blockade by conditional disruption recombination signal binding protein Jκ (RBPj cKO) significantly delayed growth subcutaneously inoculated Lewis lung (LLC), accelerated orthotopically...
Nitric oxide (NO) has many important biological functions; however, it been a long-standing challenge to utilize the exogenous NO donor itself in activation of macrophages for cancer immunotherapy. Herein, we report synthesis nanoparticle-based delivery platform with rational design effective and macrophage activation. S-Nitrosothiol (SNO) modified organosilica nanoparticles tetrasulfide-containing composition produced higher level intracellular than their bare silica counterparts...
Abstract Breast cancer can metastasize to various organs, including the lungs. The immune microenvironment of organs be metastasized plays a crucial role in metastasis breast cancer. Infection with pathogens such as viruses and bacteria alter status lung. However, effect chronic inflammation caused by on formation premetastatic niche within lung is unclear, contribution specific mediators tumor also remains largely undetermined. Here, we used mouse model revealing that pulmonary bacterial...
Abstract Background Micro/nano-textured hierarchical titanium topography is more bioactive and biomimetic than smooth, micro-textured or nano-textured topographies. Bone marrow mesenchymal stem cells (BMSCs) exosomes derived from BMSCs play important roles in the osseointegration of implants, but effects mechanisms on BMSCs-derived exosome secretion are still unclear. This study determined whether behavior differently affected by different topographies both vitro vivo. Results We found that...
Recent studies have shown that tumor-associated macrophages (TAMs) play an important role in cancer invasion and metastasis. Our previous reported TAMs promote the metastasis of gastric (GC) cells through Kindlin-2 pathway. However, mechanism needs to be clarified.THP-1 monocytes were induced by PMA/interleukin (IL)-4/IL-13 establish efficient TAM model vitro M2 isolated via flow cytometry. A dual luciferase reporter system chromatin immunoprecipitation (ChIP) assay used investigate...
Background and Aims: Nicotinamide N-methyltransferase (NNMT), an enzyme responsible for the methylation of nicotinamide, is involved in many metabolic pathways adipose tissue liver. However, role NNMT editing tumor immune microenvironment not well understood. Approach Results: Here, we identified that can promote IL6 granulocyte-macrophage colony-stimulating factor (GM-CSF) expression by decreasing tri-methyl-histone H3 levels on promoters CSF2 (encoding GM-CSF) CCAAT/Enhancer Binding...
Abstract Activation of Kupffer cells (KCs) plays a central role in the pathogenesis alcoholic liver disease (ALD). C57BL/6 mice fed EtOH-containing diet showed mixed induction hepatic classical (M1) and alternative (M2) macrophage markers. Since telomerase activation occurs at critical stages myeloid lymphoid cell activation, we herein investigated reverse transcriptase (TERT), determining factor telomerase, during ALD. In our study, TERT expression activity (TA) were remarkably increased...
Background and Aims Recent studies reveal the accumulation of myeloid derived suppressor cells (MDSCs) in human peripheral blood mononuclear (PBMCs) following HCV infection, which may facilitate maintain persistent infection. The mechanisms by induces MDSCs are poorly understood. In present study, we investigated that lead to suppression T cell proliferation expansion CD4+Foxp3+ regulatory cells. Methods Purified monocytes from healthy donors were cultured with core protein (HCVc) or...
Hepatitis C virus (HCV) establishes persistent infection in most infected patients, and eventually causes chronic hepatitis, cirrhosis, hepatocellular carcinoma some patients. Monocytes macrophages provide the first line of defense against pathogens, but their roles HCV remains unclear. We have reported that core protein (HCVc) manipulates human blood-derived dendritic cell development. In present study, we tested whether HCVc affects monocyte differentiating into macrophages. Results showed...
Macrophages exhibit an M2-pattern cytoskeleton and promote angiogenesis on the micro/nano-net.
Summary The tumour microenvironment (TME) plays a critical role in disease progression multiple myeloma (MM). This study aimed to present an atlas of MM‐TME and explore TME‐directed therapeutic strategies. We performed single‐cell RNA sequencing (scRNAseq) samples from different stages. validated the findings by bulk RNAseq, flow cytometry (FCM) vitro vivo functional experiments. delineated compromised TME during progression, characterized enrichment exhausted NK cells CD8 + T reprogramming...
Ovarian cancer is the tumor with highest mortality among gynecological malignancies. Studies have confirmed that paclitaxel chemoresistance associated increased infiltration of tumor-associated macrophages (TAMs) in microenvironment. Colony-stimulating factor 1 (CSF-1) receptor (CSF-1R) plays a key role regulating number and differentiation certain solid tumors. There are few reports on effects targeted inhibition CSF-1R combination chemotherapy ovarian Here, we explored antitumor efficacy...
Acute lung injury (ALI)/severe acute respiratory distress syndrome (ARDS) is a serious clinical characterized by high mortality rate. The pathophysiological mechanisms underlying ALI/ARDS remain incompletely understood. Considering the crucial role of immune infiltration and macrophage polarization in pathogenesis ALI/ARDS, this study aims to identify key genes associated with both M1 polarization, employing combination bioinformatics experimental approaches. findings could potentially...
Multiple myeloma is a plasma cell malignancy that thrives in the bone marrow (BM), with frequent progression to new local and distant sites. Our previous studies demonstrated multiple cells at primary sites secrete soluble factors suppress osteoblastogenesis via inhibition of Runt-related transcription factor 2 (Runx2) pre- immature osteoblasts (OB) sites, prior arrival metastatic tumor cells. However, it unknown whether OB-Runx2 suppression feeds back promote dissemination these areas....