A5051135981- Genomics and Chromatin Dynamics
- Tuberculosis Research and Epidemiology
- Nuclear Structure and Function
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Sperm and Testicular Function
- Developmental Biology and Gene Regulation
- Fungal and yeast genetics research
- Hormonal and reproductive studies
- Pneumonia and Respiratory Infections
- Prenatal Screening and Diagnostics
- Chromosomal and Genetic Variations
- Neuroendocrine regulation and behavior
- Mesenchymal stem cell research
- Pregnancy and preeclampsia studies
- Estrogen and related hormone effects
- Respiratory Support and Mechanisms
- Anesthesia and Pain Management
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Emergency and Acute Care Studies
- Hemoglobinopathies and Related Disorders
- Cancer Cells and Metastasis
- Marine Ecology and Invasive Species
- Sexual Differentiation and Disorders
Stowers Institute for Medical Research
2015-2024
Mayo Clinic in Arizona
2024
Alaska Native Medical Center
2024
University of Newcastle Australia
2022-2024
Massachusetts General Hospital
2023-2024
Harvard University
2023-2024
Florida Institute of Technology
2024
Washington University in St. Louis
2021-2023
Medical University of South Carolina
2017-2023
Clemson University
2020-2022
High density (HD) SNP genotyping arrays are an important tool for genetic analyses of animals and plants. Although the chicken is one most farm animals, no HD array yet available high resolution analysis this species. We report here development a 600 K Affymetrix® Axiom® designed using SNPs segregating in wide variety populations. In order to generate large catalogue SNPs, we re-sequenced 243 chickens from 24 lines derived diverse sources (experimental, commercial broiler layer lines) by...
Several authors have proposed that soils are made up of aggregates various sizes, stabilised by different organic and inorganic materials. Fungal hyphae been shown to bind microaggregates (<250 µm diameter) into macroaggregates (>250 diameter). This paper examines the aggregation soil clay saprophytic (Rhizoctonia solani Hyalodendron sp.) mycorrhizal (Hymenoscyphus ericae Hebeloma fungi. The results support hypothesis fungal bring mineral particles materials together form stable at...
To develop an effective and sustainable cell therapy for sickle disease (SCD), we investigated the feasibility of targeted disruption BCL11A gene, either within exon 2 or at GATAA motif in intronic erythroid-specific enhancer, using zinc finger nucleases human bone marrow (BM) CD34+ hematopoietic stem progenitor cells (HSPCs). Both targeting strategies upregulated fetal globin expression erythroid to levels predicted inhibit hemoglobin S polymerization. However, complete inactivation...
Background. Increasing access to drugs for the treatment of multidrug-resistant (MDR) tuberculosis is crucial but could lead increasing resistance these same drugs. In 2000, international Green Light Committee (GLC) initiative began increase while attempting prevent acquired resistance.
Regulation of hematopoietic stem cells (HSCs) by bone marrow (BM) niches has been extensively studied; however, whether and how HSC subpopulations are distinctively regulated BM remain unclear. Here, we functionally distinguished reserve HSCs (rHSCs) from primed (pHSCs) based on their response to chemotherapy examined they dichotomously niches. Both pHSCs rHSCs supported long-term hematopoiesis in homeostasis; were sensitive but resistant chemotherapy. Surviving restored the pool...
Abstract Background. Resistance to second-line drugs develops during treatment of multidrug-resistant (MDR) tuberculosis, but the impact on outcome has not been determined. Methods. Patients with MDR tuberculosis starting drug were enrolled in a prospective cohort study. Sputum cultures analyzed at central reference laboratory. We compared subjects successful and poor outcomes terms (1) initial acquired resistance fluoroquinolones injectable (SLIs) (2) regimens. Results. Of 1244 patients 973...
Understanding the protein composition of inner nuclear membrane (INM) is fundamental to elucidating its role in normal function and disease; however, few tools exist examine INM living cells, INM-specific proteome remains poorly characterized. Here, we adapted split green fluorescent (split-GFP) systematically localize known predicted integral proteins Saccharomyces cerevisiae as opposed outer membrane. Our data suggest that components endoplasmic reticulum (ER) well other organelles are...
The contractile ring is a complex molecular apparatus which physically divides many eukaryotic cells. Despite knowledge of its protein composition, the architecture not known. Here we have applied super-resolution microscopy and FRET to determine nanoscale spatial organization Schizosaccharomyces pombe components relative plasma membrane. Similar other membrane-tethered actin structures, find proteins localize in specific layers most membrane-proximal layer (0-80 nm) composed...
Duplication of the yeast centrosome (called spindle pole body, SPB) is thought to occur through a series discrete steps that culminate in insertion new SPB into nuclear envelope (NE). To better understand this process, we developed novel two-color structured illumination microscopy with single-particle averaging (SPA-SIM) approach study localization all 18 components during duplication using endogenously expressed fluorescent protein derivatives. The increased resolution and quantitative...
The testicular vasculature forms a complex network, providing oxygenation, micronutrients, and waste clearance from the testis. is also instrumental to testis function because it both route by which gonadotropins are delivered T transported away target organs. Whether Sertoli cells play role in regulating postnatal life has never been unequivocally demonstrated. In this study we used models of acute cell ablation germ address whether actively influence vascular structure adult Our findings...
Circadian rhythms are nearly ubiquitous throughout nature, suggesting they critical for survival in diverse environments. Organisms inhabiting largely arrhythmic environments, such as caves, offer a unique opportunity to study the evolution of circadian response changing ecological pressures. Populations Mexican tetra, Astyanax mexicanus, have repeatedly invaded caves from surface rivers, where individuals must contend with perpetual darkness, reduced food availability, and limited...
Tumor-initiating stem cells (TSCs) are critical for drug resistance and immune escape. However, the mutual regulations between TSC tumor microenvironment (TME) remain unclear. Using DNA-label retaining, single-cell RNA sequencing (scRNA-seq), other approaches, we investigated intestinal adenoma in response to chemoradiotherapy (CRT), thus identifying therapy-resistant TSCs (TrTSCs). We find bidirectional crosstalk TME using CellPhoneDB analysis. An intriguing finding is that shape into a...
Accumulating preclinical and preliminary translational evidence shows that the hypothalamic peptide oxytocin reduces food intake, increases energy expenditure, promotes weight loss. It is currently unknown whether administration effective in treating human obesity.
There is interest in developing prolonged duration local anesthesics. Here we examine whether tetrodotoxin (TTX) can be used to prolong the block from bupivacaine microspheres with and without dexamethasone. Rats received sciatic nerve blocks 75 mg of containing 0.05% (w/w) TTX, 50% and/or 0.1% TTX were also tested. The carrier fluid contained 1:100,000 epinephrine. Nociceptive motor blockade hindpaw quantified. Nerves adjacent muscles harvested 2 weeks after injection for histological...
Abstract Internalization of ligand bound G protein-coupled receptors, an important cellular function that mediates receptor desensitization, takes place via distinct pathways, which are often unique for each receptor. The C-C chemokine (CCR7) is expressed on naive T cells, dendritic and NK cells has two endogenous ligands, CCL19 CCL21. Following binding CCL21, 21 ± 4% CCR7 internalized in the HuT 78 human cell lymphoma line, while 76 8% upon to CCL19. To determine whether arrestins mediated...
The ability to break symmetry and polarize through self-organization is a fundamental feature of cellular systems. A prevailing theory in yeast posits that breaking occurs via positive feedback loop, wherein the adaptor protein Bem1 promotes local activation accumulation Cdc42 by directly tethering Cdc42GTP with its guanine nucleotide exchange factor (GEF) Cdc24. In this paper, we find neither nor bind required for cell polarization. Instead, functions primarily boosting GEF activity, role...
Following the World Health Organization's endorsement of Xpert® MTB/RIF assay, which rapidly and simultaneously diagnoses tuberculosis (TB) detects resistance to rifampin (RMP), question arises what extent RMP is an adequate marker for multidrug-resistant TB (MDR-TB). A retrospective analysis data from >81 countries subnational settings demonstrated that >40% RMP-resistant isolates new cases did not display isoniazid (INH) in with relatively low MDR-TB prevalence (one third all settings)....
The tamoxifen-inducible Cre system is a popular transgenic method for controlling the induction of recombination by at specific time and in cell type. However, tamoxifen not an inert inducer recombination, but established endocrine disruptor with mixed agonist/antagonist activity acting via endogenous estrogen receptors. Such potentially confounding effects should be controlled for, >40% publications that have used to generate conditional knockouts reported even minimum appropriate controls....