A5025418475- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Heart Rate Variability and Autonomic Control
- Vagus Nerve Stimulation Research
- Ion channel regulation and function
- Adenosine and Purinergic Signaling
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- EEG and Brain-Computer Interfaces
- Sleep and Wakefulness Research
- Neuroendocrine regulation and behavior
- Connexins and lens biology
- Cardiac electrophysiology and arrhythmias
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Pain Management and Treatment
- Nicotinic Acetylcholine Receptors Study
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Biochemical effects in animals
- Vestibular and auditory disorders
- Memory and Neural Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Pain Mechanisms and Treatments
University of Leeds
2014-2023
Faculty (United Kingdom)
2020
Leeds Dental Hospital
1998-2001
The Royal Free Hospital
1993-1999
University College London
1993-1999
DuPont (United States)
1999
Roland Hill (United Kingdom)
1999
1. In slices of rat sensorimotor cortex, dual intracellular recordings were obtained from 1,952 pairs deep layer pyramidal neurons. Where action potentials in one neurone elicited excitatory postsynaptic (EPSPs, n = 56) the other, responses to different presynaptic firing rates and patterns at membrane recorded on some occasions both neurons filled with biocytin. 2. Slices fixed, sectioned again 60 microns, incubated Avidin horseradish peroxidase (HRP), which was then visualized using...
The ionotropic ATP receptor subunits P2X<sub>1–6</sub> receptors play important roles in synaptic transmission, yet the P2X<sub>7</sub>receptor has been reported as absent from neurons normal adult brain. Here we use RT-PCR to demonstrate that transcripts for P2X<sub>7</sub> are present extracts medulla oblongata, spinal cord, and nodose ganglion. Using <i>in situ</i> hybridization mRNA encoding, was detected numerous throughout oblongata cord. Localizing protein with immunohistochemistry...
1. Using dual intracellular recordings in slices of adult rat neocortex, twenty-four IPSPs activated by single presynaptic interneurones were studied simultaneously recorded pyramidal cells. Fast spiking inhibited one four or five their close neighbours. No reciprocal connections observed. After neurones filled with biocytin. 2. Interneurones that elicited classified as classical fast (n = 10), non-classical 3, including burst-firing interneurone), unclassified, slow 8), regular 3), i.e....
Abstract Although originally cloned from rat brain, the P2X 7 receptor has only recently been localized in neurones, and functional responses mediated by these neuronal receptors (P2X R) are largely unknown. Here we studied effect of R activation on release neurotransmitters superfused hippocampal slices. ATP (1–30 m ) other analogues elicited concentration‐dependent [ 3 H]GABA outflow, with following rank order potency: benzoylbenzoylATP (BzATP) > ADP. PPADS, non‐selective P2‐receptor...
The integration of somatosensory information is generally assumed to be a function the central nervous system (CNS). Here we describe fully functional GABAergic communication within rodent peripheral sensory ganglia and show that it can modulate transmission pain-related signals from nerves CNS. We found neurons express major proteins necessary for GABA synthesis release released in response depolarization. In vivo focal infusion or reuptake inhibitor dramatically reduced acute peripherally...
Ageing is associated with attenuated autonomic function. Transcutaneous vagal nerve stimulation (tVNS) improved function in healthy young participants. We therefore investigated the effects of a single session tVNS (studies 1 and 2) administered daily for two weeks (study 3) volunteers aged ≥ 55 years. was performed using modified surface electrodes on tragus connected to transcutaneous electrical (TENS) machine. Study 1: participants (n=14) received sham. 2: all (n=51) underwent tVNS. 3:...
Dual intracellular recordings in the CA1 region of adult rat hippocampal slices and biocytin filling synaptically connected cells were used to study excitatory postsynaptic potentials (EPSPs) elicited basket ( n = 7 ) bistratified interneurones by action activated simultaneously recorded pyramidal cells. Interneurones could be subdivided according their electrophysiological properties into classical fast spiking, burst firing, regular spiking with a rounded spike after‐hyperpolarization....
1 Angiotensin II (ANGII) acting on ANGII type (AT1) receptors in the solitary tract nucleus (NTS) depresses baroreflex. Since stimulates release of nitric oxide (NO), we tested whether ANGII-mediated depression baroreflex NTS depended NO release. 2 In a working heart-brainstem preparation (WHBP) rat microinjection either (500 fmol) or donor (diethylamine nonoate, 500 pmol) both depressed gain by -56 and -67 %, respectively (P < 0.01). contrast, whilst potentiated peripheral chemoreflex, was...
1. Double intracellular recordings were made from 1163 pairs of pyramidal neurones in layer V-VI the rat somatomotor cortex vitro using sharp electrodes filled with biocytin. Monosynaptically connected cells identified when an action potential one could elicit a constant latency excitatory postsynaptic (EPSP) other and Labelled subsequently histologically avidin-horseradish peroxidase. 2. Thirty-four found to be monosynaptically connected. Fifteen these sufficiently stable for...
Differentiation of pluripotent embryonic stem (ES) cells through multipotent neural (NS) into differentiated neurons is accompanied by wholesale changes in transcriptional programs. One factor that present at all three stages and a key to neuronal differentiation the RE1-silencing transcription (REST/NRSF). Here, we have used novel chromatin immunoprecipitation-based cloning strategy (SACHI) identify 89 REST target genes ES cells, hippocampal NS mature hippocampus. The gene products are...
Abstract The origin of cardiac vagal preganglionic motoneurones in the rat is still controversial and knowledge chemistry synaptic inputs onto these neurones limited. In this investigation innervating heart were identified by retrograde transport cholera toxin conjugated to horseradish peroxidase (CT‐HRP) combined with immunocytochemical localization 5‐hydroxytryptamine. Injection CT‐HRP into myocardium resulted labelling primarily ventral regions nucleus ambiguus (75.1%). Labelled also...
The Na<sup>+</sup>/K<sup>+</sup> ATPase (NKA) is an essential membrane protein underlying the potential in excitable cells. Transmembrane ion transport performed by catalytic α subunits (α1–4). predominant neurons are α1 and α3, which have different affinities for Na<sup>+</sup> K<sup>+</sup>, impacting on kinetics. exchange rate of markedly influences activity expressing them. We investigated distribution function main isoforms subunit expressed mouse spinal cord. NKAα1 immunoreactivity...
Fast inhibition in the nervous system is commonly mediated by GABA(A) receptors comprised of 2alpha/2beta/1gamma subunits. In contrast, GABA(C) containing only rho subunits (rho1-rho3) have been predominantly detected retina. However, here using reverse transcription-PCR and situ hybridization we show that mRNA encoding rho1 subunit highly expressed brainstem neurons. Immunohistochemistry localized to neurons at light electron microscopic levels, where it was synaptic junctions. Application...