A5103199865- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Hypothalamic control of reproductive hormones
- Neurogenesis and neuroplasticity mechanisms
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Muscle Physiology and Disorders
- Animal Nutrition and Physiology
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Viral Infectious Diseases and Gene Expression in Insects
- Congenital heart defects research
- Neuroscience and Neural Engineering
- Cardiomyopathy and Myosin Studies
- Regulation of Appetite and Obesity
- Tissue Engineering and Regenerative Medicine
- Silk-based biomaterials and applications
- Circadian rhythm and melatonin
- Neural dynamics and brain function
- TGF-β signaling in diseases
- Biochemical Analysis and Sensing Techniques
- Wnt/β-catenin signaling in development and cancer
Masaryk University
2013-2022
King's College London
2008-2010
National Central University
2006
University of Leeds
2003-2005
University of Cape Town
1998-2003
Roslin Institute
2001
University of Glasgow
2000
Kaohsiung Medical University
1991
Differentiation of pluripotent embryonic stem (ES) cells through multipotent neural (NS) into differentiated neurons is accompanied by wholesale changes in transcriptional programs. One factor that present at all three stages and a key to neuronal differentiation the RE1-silencing transcription (REST/NRSF). Here, we have used novel chromatin immunoprecipitation-based cloning strategy (SACHI) identify 89 REST target genes ES cells, hippocampal NS mature hippocampus. The gene products are...
Abstract Neural induction is the first step in formation of vertebrate central nervous system. The emerging consensus mechanisms underling neural combined influences from inhibiting bone morphogenetic protein (BMP) signaling and activating fibroblast growth factor (FGF)/Erk signaling, which act extrinsically via either autocrine or paracrine fashions. However, do intrinsic forces (cues) exist they play decisive roles induction? These questions remain to be answered. Here, we have identified...
ABSTRACT Neural rosette formation is a critical morphogenetic process during neural development, whereby stem cells are enclosed in niches to equipoise proliferation and differentiation. How rosettes form provide regulatory micro-environment remains be elucidated. We employed the human embryonic cell-based system investigate structural development function of rosettes. Our study shows that consists five types morphological change: intercalation, constriction, polarization, elongation lumen...
The cellular pathology of schizophrenia and the potential antipsychotics to target underlying neuronal dysfunctions are still largely unknown. We employed glutamatergic neurons derived from induced pluripotent stem cells (iPSC) obtained patients with known histories response clozapine healthy controls decipher mechanisms action clozapine, spanning molecular (transcriptomic profiling) (electrophysiology) levels observed clinical effects in living patients. Glutamatergic exhibited deficits...
Mammalian receptors for gonadotropin-releasing hormone (GnRH) have over 85% sequence homology and similar ligand selectivity. Biological studies indicated that the chicken GnRH receptor has a distinct pharmacology, certain antagonists of mammalian function as agonists. To explore structural determinants this, we cloned pituitary demonstrated it marked differences in primary amino acid (59% homology) its interactions with analogs. The had high affinity (K i 4.1 ± 1.2 nm) , to human 4.8 nm)....
Astrocytes are the most abundant cell type in human brain and important regulators of several critical cellular functions, including synaptic transmission. Although astrocytes known to play a central role etiology pathophysiology schizophrenia, little is about their potential involvement clinical response antipsychotic clozapine. Moreover, display remarkable degree morphological diversity, but contribution astrocytic subtypes disease biology drug has received attention. Here, we used...
Neural development from blastocysts is strictly controlled by intricate transcriptional programmes that initiate the down-regulation of pluripotent genes, Oct4, Nanog and Rex1 in followed up-regulation lineage-specific genes as neural proceeds. Here, we demonstrate expression pattern transcription factor Rest mirrors those during embryonic stem (ES) cells an early abrogation ES using a combination gene targeting RNAi approaches causes defects this process. Specifically, ablation does not...
The aim of this study was to increase understanding the occurrence and regulation chicken gonadotropin releasing hormone I (cGnRH I) receptor (cGnRH-R) mRNA variants in hypothalamic-pituitary-testicular axis (HPTA). carried out cockerel. Fully processed cGnRH Ia) a variant transcript Ib) with retained intron 1 were observed preoptic/anterior hypothalamus (POA), basal hypothalamus, anterior pituitary gland, testes. cGnRH-R (cGnRH-Ra) (cGnRH-Rb) deletion detected same tissues. In juvenile...
This study elucidated the stage-specific roles of FGF2 signaling during neural development using in-vitro human embryonic stem cell-based developmental modeling. We found that dysregulation prior to onset induction resulted in malformation rosettes (a tube-like structure), despite cells having undergone induction. The aberrant rosette formation may be attributed misplacement ZO-1, which is a polarized tight junction protein and shown co-localized with FGF2/FGFR1 apical region rosettes,...
The chicken GnRH receptor (cGnRH-R) differs from all mammalian GnRH-Rs in possessing a cytoplasmic carboxyl-terminal tail. We have previously demonstrated that the cGnRH-R undergoes more rapid agonist-induced internalization than and requires tail for this process. To investigate structural determinants mediating internalization, series of mutant receptors was generated, including progressive truncations substitution serine threonine residues with alanine. Truncation to position 366 then 356...
We report on the cloning of full-length complementary DNA for chicken TRH receptor. Although receptor has been cloned from several mammalian species, this is first another vertebrate class. The ligand binding pocket, which situated in transmembrane helices mouse and rat receptors, completely conserved Pharmacological studies (receptor signaling) employing analogs revealed that there are no significant differences between receptors. These findings show have considerable evolutionary...
In late sepsis, it has been established that the liver plays a major role in initiation of multiorgan failure, which is most lethal complication hospitals. The molecular mechanism underlying failure results from sepsis remains elusive. This study was undertaken to identify bona fide differentially expressed genes 18-h septic by suppression subtractive hybridization, and data were corroborated Northern blot analysis. differential gene expression profile renders clue as involved failure. cecal...
Identification of transcriptional regulatory sites plays an important role in the investigation gene regulation. For this propose, we designed and implemented a data warehouse to integrate multiple heterogeneous biological sources with types such as text-file, XML, image, MySQL database model, Oracle model. The utility predicting coregulated genes was explored using synexpression group derived from microarray study. Both binding known transcription factors predicted over-represented (OR)...
Human pluripotent stem cell (hPSC)-based modeling offers the potential for studying human diseases using systems. An increasing number of studies in numerous fields demonstrate that hPSC-based disease systems capture specific pathophysiology occurring vivo. A widespread deployment hPSC is foreseeable. Even field psychiatric disorders (for example, schizophrenia and autism), which lags behind due to complex underlying causes, such as inaccessibility brain cells assessments absence reliable...