A5036303622- SARS-CoV-2 and COVID-19 Research
- Rabies epidemiology and control
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Fiber-reinforced polymer composites
- Wind and Air Flow Studies
- Cardiac electrophysiology and arrhythmias
- Aerodynamics and Fluid Dynamics Research
- Bacillus and Francisella bacterial research
- Graphite, nuclear technology, radiation studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Neuroscience and Neuropharmacology Research
- Respiratory viral infections research
- Graphene research and applications
- Poxvirus research and outbreaks
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Water Quality Monitoring and Analysis
- Soil Mechanics and Vehicle Dynamics
- Cardiac Fibrosis and Remodeling
- Research in Cotton Cultivation
- Signaling Pathways in Disease
- Hepatitis B Virus Studies
Harbin Veterinary Research Institute
2012-2024
Chinese Academy of Agricultural Sciences
2012-2024
Shenzhen University
2019-2024
Tsinghua University
2022-2023
Xi'an Technological University
2023
Beijing University of Chemical Technology
2015-2022
Beijing Institute of Aeronautical Materials
2022
Wanfang Data (China)
2022
Fujian University of Technology
2022
State Key Laboratory of Automotive Simulation and Control
2021
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) causes the infectious disease COVID-19 (coronavirus 2019), which was first reported in Wuhan, China, December 2019. Despite extensive efforts to control disease, has now spread more than 100 countries and caused a global pandemic. SARS-CoV-2 is thought have originated bats; however, intermediate animal sources of virus are unknown. In this study, we investigated susceptibility ferrets animals close contact with humans SARS-CoV-2....
Abstract The unprecedented coronavirus disease 2019 (COVID-19) epidemic has created a worldwide public health emergency, and there is an urgent need to develop effective vaccine control this severe infectious disease. Here, we find that single vaccination with replication-defective human type 5 adenovirus encoding the SARS-CoV-2 spike protein (Ad5-nCoV) protect mice completely against mouse-adapted infection in upper lower respiratory tracts. Additionally, Ad5-nCoV protects ferrets from...
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important target for vaccine and drug development. However, the rapid emergence variant strains with mutated S proteins has rendered many treatments ineffective. Cleavage by host proteases essential viral infection. Here, we discovered that contains two previously unidentified Cathepsin L (CTSL) cleavage sites (CS-1 CS-2). Both are highly conserved among all known SARS-CoV-2 variants. Our structural...
The 3C-like protease (3CLpro) is an essential enzyme for the replication of SARS-CoV-2 and other coronaviruses thus a target coronavirus drug discovery. Nearly all inhibitors 3CLpro reported so far are covalent inhibitors. Here, we report development specific, noncovalent 3CLpro. most potent one, WU-04, effectively blocks replications in human cells with EC50 values 10-nM range. WU-04 also inhibits SARS-CoV MERS-CoV high potency, indicating that it pan-inhibitor showed anti-SARS-CoV-2...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the infectious disease COVID-19, which was first reported in Wuhan, China December, 2019. Despite tremendous efforts to control disease, COVID-19 has now spread over 100 countries and caused a global pandemic. SARS-CoV-2 is thought have originated bats; however, intermediate animal sources of virus are completely unknown. Here, we investigated susceptibility ferrets animals close contact with humans SARS-CoV-2. We...
Rabies virus (RABV) invades the central nervous system and nearly always causes fatal disease in humans. How RABV interacts with host neuron membrane receptors to become internalized cause rabid symptoms is not yet fully understood. Here, we identified a novel receptor of RABV, which uses infect neurons. We found that metabotropic glutamate subtype 2 (mGluR2), member G protein-coupled family abundant system, directly glycoprotein mediate entry. infection was drastically decreased after...
Minks are raised in many countries and have transmitted severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to humans. However, the biologic properties of SARS-CoV-2 minks largely unknown. Here, we investigated found that replicates efficiently both upper lower tracts, transmits via droplets; pulmonary lesions caused by similar those seen humans with COVID-19. We further a spike protein-based subunit vaccine prevented replication lung damage infection minks. Our study indicates...
The unprecedented coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), is a serious threat to global public health. Development of effective therapies against SARS-CoV-2 urgently needed. Here, we evaluated the antiviral activity remdesivir parent nucleotide analog, GS441524, which targets RNA-dependent RNA polymerase enzyme, and feline prodrug, GC376, its main protease, using mouse-adapted infected mouse model. Our results showed that...
Abstract Outbreaks of coronaviruses (CoVs), especially severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), have posed serious threats to humans and animals, which urgently calls for effective broad‐spectrum antivirals. RNA‐dependent RNA polymerase (RdRp) plays an essential role in viral synthesis is ideal pan‐coronaviral therapeutic target. Herein, based on cryo‐electron microscopy biochemical approaches, gossypol (GOS) identified from 881 natural products directly block SARS‐CoV‐2...
Forced ventilation is typically used in the construction of tunnels since it an economical method to provide high amounts fresh air. Air velocity and pollutant concentration near work face are determined by arrangement. In study, field measurement air was performed, computational fluid dynamics (CFD) models were constructed investigate airflow predict gas distribution a tunnel construction. The effects distance between duct exit (L1) evaluated analyzing flow concentration. evaluation shows...
Rabies virus (RABV) invades the central nervous system and nearly always causes fatal disease in humans. RABV enters cells via clathrin-mediated endocytosis upon receptor binding. The detailed mechanism of this process how it is regulated are not fully understood. Here, we carried out a high-through-put RNAi analysis identified AP2-associated kinase 1 (AAK1), serine/threonine kinase, as an important cellular component regulating entry RABV. AAK1 knock-down greatly inhibits infection cells,...
The continuous emergence of severe acute respiratory coronavirus 2 (SARS-CoV-2) variants and the increasing number breakthrough infection cases among vaccinated people support urgent need for research development antiviral drugs. Viral entry is an intriguing target drug development. We found that diltiazem, a blocker L-type calcium channel Cav1.2 pore-forming subunit (Cav1.2 α1c) FDA-approved drug, inhibits binding internalization SARS-CoV-2, decreases SARS-CoV-2 in cells mouse lung. α1c...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses angiotensin-converting enzyme (ACE2) as a binding receptor to enter cells via clathrin-mediated endocytosis (CME). However, receptors involved in other steps of SARS-CoV-2 infection remain largely unknown. Here, we found that metabotropic glutamate subtype (mGluR2) is an internalization factor for SARS-CoV-2. Our results show mGluR2 directly interacts with the spike protein and knockdown decreases but not cell binding....
For most viruses, cell entry involves engagement with many distinct plasma membrane components, each of which is essential. After binding to its specific receptor(s), rabies virus (RABV) enters host cells through the process clathrin-mediated endocytosis.
ABSTRACT The formation process and mechanism of the pseudo‐graphite sheets polyacrylonitrile (PAN) during thermal treatment in inert atmosphere were investigated by thermo‐gravimetry (TG) X‐ray diffraction (XRD). According to results, conjugated plane originally formed stabilization was proposed as basic planar structure sheets, it connected stacked form microcrystal through chain scission reaction non‐carbon atoms elimination 280–450 °C 800–1300 respectively. situ measurements for time...
Rabies virus (RABV) is a widespread pathogen that causes fatal disease in humans and animals. It has been suggested multiple host factors are involved RABV entry. Here, we showed uses integrin β1 (ITGB1) for cellular infection was drastically decreased after ITGB1 short interfering RNA knockdown moderately increased overexpression cells. directly interacts with glycoprotein. Upon infection, internalized into cells transported to late endosomes together RABV. The infectivity of cell-adapted...
We previously found that metabotropic glutamate receptor subtype 2 (mGluR2) is an entry for RABV in vitro , and important internalization factor SARS-CoV-2 vivo . However, whether mGluR2 required infection was unknown.
Rabies virus (RABV) matrix protein (M) plays crucial roles in viral transcription, replication, assembly, and budding; however, its function during the early stage of replication remains unknown. Here, we mapped interactome between RABV M human host factors using a proteomic approach, finding link to V-type proton ATPase catalytic subunit A (ATP6V1A), which is located endosomes where first enters. By downregulating or upregulating ATP6V1A expression HEK293T cells, found that facilitated...