A5012815949- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Silicon and Solar Cell Technologies
- Thin-Film Transistor Technologies
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Respiratory viral infections research
- Mechanical Behavior of Composites
- Semiconductor materials and interfaces
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- Electrochemical sensors and biosensors
- Textile materials and evaluations
- Solar Thermal and Photovoltaic Systems
- Spaceflight effects on biology
- Rabies epidemiology and control
- Conducting polymers and applications
- Bone health and osteoporosis research
- CRISPR and Genetic Engineering
- Viral Infections and Outbreaks Research
- Anesthesia and Neurotoxicity Research
- Iron and Steelmaking Processes
Harbin Veterinary Research Institute
2012-2025
Chinese Academy of Agricultural Sciences
2010-2025
Huaneng Clean Energy Research Institute
2022-2024
Tokyo University of Science
2015-2024
Xinjiang Agricultural University
2023-2024
The University of Tokyo
2015-2024
China Mobile (China)
2024
Ansteel (China)
2020-2024
Beihua University
2019-2023
China Huaneng Group Co., Ltd. (China)
2021-2023
Unveiling African swine fever virus (ASFV) is highly contagious and often lethal. With no vaccine or effective treatment, infections require large-scale culling of pigs. Wang et al. apply cutting-edge cryo–electron microscopy techniques to determine the structure this very large DNA virus. An 8.8-angstrom-resolution reconstruction shows five layers virus, fourth capsid layer could be reconstructed at 4.8-angstrom resolution. The reveals epitopes in major protein that distinguish ASFV from...
African swine fever (ASF) entered China in August 2018 and rapidly spread across the entire country, severely threatening Chinese domestic pig population, which accounts for more than 50% of population worldwide. In this study, an ASFV isolate, Pig/Heilongjiang/2018 (Pig/HLJ/18), was isolated primary porcine alveolar macrophages (PAMs) from a sample ASF outbreak farm. The isolate characterized by using haemadsorption (HAD) test, Western blotting immunofluorescence, electronic microscopy....
The Georgia-07-like genotype II African swine fever virus (ASFV) with high virulence has been prevalent in China since 2018. Here, we report that I ASFVs have now also emerged China. Two non-haemadsorbing ASFVs, HeN/ZZ-P1/21 and SD/DY-I/21, were isolated from pig farms Henan Shandong province, respectively. Phylogenetic analysis of the whole genome sequences suggested both isolates share similarity NH/P68 OURT88/3, two Portugal last century. Animal challenge testing revealed SD/DY-I/21 shows...
Inflammatory factors and type I interferons (IFNs) are key components of host antiviral innate immune responses, which can be released from the pathogen-infected macrophages. African swine fever virus (ASFV) has developed various strategies to evade including alteration inflammatory responses IFNs production. However, molecular mechanism underlying inhibition production by ASFV-encoded proteins not been fully understood. Here we report that ASFV infection only induced low levels IL-1β in...
Abstract African swine fever virus (ASFV) poses a great threat to the global pig industry and food security. Currently, 24 ASFV genotypes have been reported but it is unclear whether recombination of different genotype viruses occurs in nature. In this study, we detect three recombinants I II ASFVs pigs China. These are genetically similar classified as according their B646L gene, yet 10 discrete fragments accounting for over 56% genomes derived from virus. Animal studies with one...
Background and Purpose— Ischemic postconditioning (Postcond) is defined as a series of intermittent interruptions blood flow in the early phase reperfusion that mechanically alters hydrodynamics reperfusion. A recent study showed Postcond reduced infarct size cerebral ischemia/reperfusion (I/R) injury. However, little known about mechanisms I/R In present study, we investigated effects focal injury rat middle artery occlusion model. Methods— Adult male Sprague-Dawley rats were treated with...
Despite substantial efforts to control H5N1 avian influenza viruses (AIVs), the have continued evolve and cause disease outbreaks in poultry infections humans. In this report, we analyzed 51 representative AIVs isolated from domestic poultry, wild birds, humans China during 2004 2009, 21 genotypes were detected based on whole-genome sequences. Twelve of southern bear similar H5 hemagglutinin (HA) genes (clade 2.3). These did not display antigenic drift could be completely protected against...
ABSTRACT Novel avian-origin influenza A(H7N9) viruses were first reported to infect humans in March 2013. To date, 143 human cases, including 45 deaths, have been recorded. By using sequence comparisons and phylogenetic ancestral inference analyses, we identified several distinct amino acids the polymerase PA protein, some of which may be mammalian adapting. Mutant possessing these acid changes, singly or combination, assessed for their activities growth kinetics avian cells virulence mice....
Abstract Seasonal influenza A viruses cause annual epidemics of respiratory disease; highly pathogenic avian H5N1 and the recently emerged H7N9 severe infections in humans, often with fatal outcomes. Although numerous studies have addressed pathogenicity viruses, pathogenesis remains incompletely understood. Here we generate expressing fluorescent proteins different colours (‘Color-flu’ viruses) to facilitate study viral infection vivo models. On adaptation mice, stable expression infected...
Abstract African swine fever virus (ASFV) is a dsDNA responsible for severe, highly contagious, and lethal disease affecting both domestic wild pigs. ASFV has brought enormous economic loss to number of countries, effective vaccine therapy are still lacking. Therefore, rapid, sensitive, field-deployable detection important surveillance control. Herein, we developed Cas12a-mediated portable paper assay rapidly precisely detect ASFV. We identified robust set crRNAs that recognized the...
African swine fever (ASF) is a highly contagious infectious disease of domestic pigs and wild boars caused by virus (ASFV), with mortality rate up to 100%. In order replicate efficiently in macrophages monocytes, ASFV has evolved multiple strategies evade host antiviral responses. However, the underlying molecular mechanisms which ASFV-encoded proteins execute immune evasion are not fully understood. this study, we found that pH240R strongly inhibits transcription, maturation, secretion...
Genetic changes have occurred in the genomes of prevalent African swine fever viruses (ASFVs) field China, which may change their antigenic properties and result immune escape. There is usually poor cross-protection between heterogonous isolates, and, therefore, it important to test live attenuated ASFV vaccines against current isolates. In this study, we evaluated protective efficacy vaccine candidate HLJ/18-7GD emerging provided protection a highly virulent variant lower lethal isolate,...
African swine fever (ASF) is an acute, hemorrhagic, and severe infectious disease caused by the ASF virus (ASFV). ASFV has evolved multiple strategies to escape host antiviral immune responses. Here, we reported that pB318L, a trans-geranylgeranyl-diphosphate synthase, reduced expression of type I interferon (IFN-I) IFN-stimulated genes (ISGs). Mechanically, pB318L not only interacted with STING reduce translocation from endoplasmic reticulum Golgi apparatus but also IFN receptors...
During their circulation in nature, H5N1 avian influenza viruses (AIVs) have acquired the ability to kill natural hosts, wild birds and ducks. The genetic determinants for this increased virulence are largely unknown. In study, we compared two genetically similar AIVs, A/duck/Hubei/49/05 (DK/49) A/goose/Hubei/65/05 (GS/65), that lethal chickens but differ levels To explore basis difference virulence, generated a series of reassortants mutants these viruses. reassortant bearing PA gene from...
In order to address the resource service optimal-selection (RSOS) and composition problem in manufacturing grid (MGrid) system provide high-quality users, an MGrid RSOS framework (MGrid-RSOSCF) is investigated this study. The process of divided into following five steps MGrid-RSOSCF: (1) decomposing submitted task several subtasks (i.e. single requested task) if a multiple task; (2) searching out qualified for each decomposed subtask generating corresponding candidate set; (3) retrieving,...
Huntington's Disease (HD) is a progressive neurodegenerative disorder with single causal mutation in the Huntingtin (HTT) gene. MicroRNAs (miRNAs) have recently been implicated as epigenetic regulators of neurological disorders, however, their role HD pathogenesis not well defined. Here we study transgenic monkeys (HD monkeys) to examine miRNA dysregulation primate model disease. In this report, 11 miRNAs were found be significantly associated (P value < 0.05) frontal cortex monkeys. We...
African swine fever is a widespread and highly contagious disease in the porcine population, which caused by virus (ASFV). The PCR ELISA detection methods are main conventional diagnostic for ASFV antigen/antibody field. However, these have limitations of expensive equipment, trained technicians, time-consuming results. Thus, rapid, inexpensive, accurate on-site method urgently needed. Here we describe double-antigen-sandwich lateral-flow assay based on gold nanoparticle-conjugated major...