A5087576897- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Magnetic properties of thin films
- Viral Infections and Vectors
- Copper Interconnects and Reliability
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Semiconductor materials and devices
- Receptor Mechanisms and Signaling
- Genomics and Chromatin Dynamics
- Erythrocyte Function and Pathophysiology
- Inflammatory mediators and NSAID effects
- Visual perception and processing mechanisms
- Retinal Development and Disorders
- interferon and immune responses
- Estrogen and related hormone effects
- Complement system in diseases
- Animal Disease Management and Epidemiology
- Fullerene Chemistry and Applications
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Peptidase Inhibition and Analysis
- Plant Virus Research Studies
- Characterization and Applications of Magnetic Nanoparticles
Kyoto University
1995-2025
Okinawa Institute of Science and Technology Graduate University
2016-2022
Osaka University
1991-2022
Protein Research Foundation
2021-2022
Tokyo University of Science
2012-2015
Hokkaido University
2015
The University of Tokyo
2011-2014
Kanazawa University
2009
Panasonic (Japan)
1997-2002
Kyoto University Institute for Chemical Research
1997
The influenza A virus genome consists of eight single-stranded negative-sense RNA (vRNA) segments. Although segmentation provides advantages such as genetic reassortment, which contributes to the emergence novel strains with pandemic potential, it complicates packaging progeny virions. Here we elucidate, using electron tomography, three-dimensional structure ribonucleoprotein complexes (RNPs) within Each virion is packed well-organized RNPs that possess rod-like structures different lengths....
Exosomes regulate cell-cell communication by transferring functional proteins and RNAs between cells. Here, to clarify the function of exosomes during influenza virus infection, we characterized lung-derived exosomal microRNAs (miRNAs). Among detected miRNAs, miR-483-3p was present at high levels in bronchoalveolar lavage fluid (BALF) infection mice with various strains virus, transfection potentiated gene expression type I interferon proinflammatory cytokine upon viral MLE-12 RNF5, a...
Abstract Histamine is a biogenic amine that participates in allergic and inflammatory processes by stimulating histamine receptors. The H 4 receptor (H R) potential therapeutic target for chronic diseases such as asthma atopic dermatitis. Here, we show the cryo-electron microscopy structures of R-G q complex bound with an endogenous agonist or selective imetit orthosteric binding pocket. demonstrate mode agonists subtype-selective causes conformational changes Phe344 7.39 , which, turn, form...
The Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain helical nucleocapsid responsible for genome transcription, replication, and packaging into progeny virions. consists nucleoprotein (NP)–viral genomic RNA complex forming core structure, to which VP24 VP35 bind externally. Two NPs, each paired with molecule, constitute repeating unit. However, detailed structure remains unclear. Here, we determine nucleocapsid-like...
Signal transducer and activator of transcription (STAT) family members mediate signaling in the Janus kinase (JAK)–STAT pathway are activated by phosphorylation at a conserved tyrosine residue, resulting dimerization through reciprocal interactions between phosphotyrosine Src homology 2 (SH2) domain. Tyrosine-phosphorylated STAT (pY-STAT) then translocates to nucleus induce expression genes encoding antiviral proteins. Although active functional forms STATs conventionally considered be...
In our previous study, we genetically analyzed bovine viral diarrhea viruses (BVDVs) isolated from 2000 to 2006 in Japan and reported that subgenotype 1b were predominant. the present 766 BVDVs 2014 Hokkaido, Japan, understand recent epidemics. Phylogenetic analysis based on nucleotide sequences of 5′-untranslated region genome revealed isolates classified as genotype 1 (BVDV-1; 544 isolates) 2 (BVDV-2; 222). BVDV-1 further divided into BVDV-1a (93), (371) 1c (80) subgenotypes, all BVDV-2...
The influenza A virus possesses an eight-segmented, negative-sense, single-stranded RNA genome (vRNA). Each vRNA segment binds to multiple copies of viral nucleoproteins and a small number heterotrimeric polymerase complexes form rod-like ribonucleoprotein complex (RNP), which is essential for the transcription replication vRNAs. However, how RNPs are organized within progeny virion not fully understood. Here, by focusing on complexes, we analyzed fine structure purified their configuration...
Abstract The nucleoprotein (NP) of Marburg virus (MARV), a close relative Ebola (EBOV), encapsidates the single-stranded, negative-sense viral genomic RNA (vRNA) to form helical NP–RNA complex. complex constitutes core structure for assembly nucleocapsid that is responsible synthesis. Although appropriate interactions among NPs and are required formation nucleocapsid, structural basis remains largely elusive. Here, we show MARV determined using cryo-electron microscopy at resolution 3.1 Å....
Abstract The single-stranded, negative-sense, viral genomic RNA (vRNA) of influenza A virus is encapsidated by nucleoproteins (NPs) and an polymerase to form a ribonucleoprotein complex (vRNP) with helical, rod-shaped structure. vRNP responsible for transcription replication the vRNA. However, conformation during synthesis not well understood. Here, using high-speed atomic force microscopy cryo-electron microscopy, we investigated native structure vRNPs in vitro. Two distinct types were...
Negatively stained influenza virions sometimes show irregular morphology and are often referred to as pleomorphic. However, this has not been visualized when ultrathin-section transmission scanning electron microscopies used. This study focused on the effects of ultracentrifugation A virion morphology, negative staining involves concentrate or purify virions. The morphologies unfixed, glutaraldehyde-fixed osmium tetroxide-fixed were quantitatively compared before after ultracentrifugation,...
Prostaglandin receptors have been implicated in a wide range of functions, including inflammation, immune response, reproduction, and cancer. Our group has previously determined the crystal structure active-like EP3 bound to its endogenous agonist, prostaglandin E2. Here, we present single-particle cryoelectron microscopy (cryo-EM) human EP3-Gi signaling complex at resolution 3.4 Å. The reveals binding mode Gi structural changes induced by binding. In addition, compare with other subtypes...
The Ebola virus, a member of the Filoviridae family, causes severe hemorrhagic fever in humans. Filamentous virions contain helical nucleocapsid responsible for genome transcription, replication, and packaging into progeny virions. consists nucleoprotein (NP)-viral genomic RNA complex forming core structure, to which VP24 VP35 bind externally. Two NPs, each paired with molecule, constitute repeating unit. However, detailed structure remains unclear. Here, we determined nucleocapsid-like...
Human vesicular monoamine transporter 2 (VMAT2), a member of the SLC18 family, plays crucial role in regulating neurotransmitters brain by facilitating their uptake and storage within vesicles, preparing them for exocytotic release. Because its central neurotransmitter signalling neuroprotection, VMAT2 is target neurodegenerative diseases movement disorders, with inhibitor being used as therapeutics. Despite importance pharmacophysiology, molecular basis VMAT2-mediated transport inhibition...
ABSTRACT In measles virus (MV)-infected cells, the ribonucleoprotein (RNP) complex, comprised of viral genome and nucleocapsid (N) protein, phosphoprotein (P protein), large assembles at perinuclear region synthesizes RNAs. The cellular proteins involved in formation RNP complex are largely unknown. this report, we show that cofilin, an actin-modulating host interacts with MV N protein aids complex. Knockdown cofilin using short hairpin RNA reduces after infection complex-like structure...
<title>Abstract</title> Recent advances in protein design have ushered an era of constructing intricate higher-order structures. Nonetheless, orchestrating the assembly diverse units into cohesive artificial structures akin to biological systems, especially tubular forms, remains elusive. To this end, here, we introduce Nature-Inspired Protein Assembly Design (NIPAD), a novel methodology that utilises two distinct create unique under carefully designed conditions. These demonstrate dynamic...
Nifedipine-induced gingival overgrowth is known to be characterized by fibrosis and some degree of inflammation. However, the molecular mechanism not fully understood. The purpose this study was investigate in vitro effects nifedipine interleukin-1alpha on molecules involved fibrosis, namely type I collagen, matrix metalloproteinase-1 (MMP-1), tissue inhibitor (TIMP-1).Four human fibroblast strains, derived from four healthy volunteers, were cultured media containing (1 microg/ml), with or...