A5100452284- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Spectroscopy and Chemometric Analyses
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Advanced Biosensing Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Electrochemical sensors and biosensors
- Water Quality Monitoring and Analysis
- RNA modifications and cancer
- Advanced Photocatalysis Techniques
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Analytical Chemistry and Sensors
- Circular RNAs in diseases
- Molecular Junctions and Nanostructures
- Image and Signal Denoising Methods
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Metal-Organic Frameworks: Synthesis and Applications
- Cancer-related molecular mechanisms research
- Groundwater and Isotope Geochemistry
Linyi University
2023-2025
Hebei University of Technology
2003-2025
Nanjing Medical University
2025
Xi’an University of Posts and Telecommunications
2025
Ningxia Medical University
2022-2025
Tongji University
2013-2024
Shanghai Tenth People's Hospital
2013-2024
Jining University
2013-2024
China National Petroleum Corporation (China)
2024
Chifeng University
2024
A new strategy for dramatically amplifying enzyme-linked electrical detection of proteins and DNA using carbon nanotubes (CNTs) carrying numerous enzyme tracers accumulating the enzymatically liberated product on CNT-modified transducer is described. Such a CNT-derived double-step amplification pathway (of both recognition transduction events) allows down to 1.3 160 zmol, respectively, in 25−50 μL samples indicates great promise PCR-free analysis. The protocol illustrated monitoring sandwich...
Nucleic-acid hybridization assays based on the use of different inorganic-colloid (quantum dots) nanocrystal tracers for simultaneous electrochemical measurements multiple DNA targets are described. Three encoding nanoparticles (zinc sulfide, cadmium and lead sulfide) used to differentiate signals three in connection stripping-voltammetric heavy metal dissolution products. These products yield well-defined resolved stripping peaks at −1.12 V (Zn), −0.68 (Cd), −0.53 (Pb) mercury-coated...
The concept of ligand bias at G protein-coupled receptors broadens the possibilities for agonist activities and provides opportunity to develop safer, more selective therapeutics. Morphine pharmacology in β-arrestin-2 knockout mice suggested that a promotes coupling μ-opioid receptor (MOR) proteins, but not β-arrestins, would result higher analgesic efficacy, less gastrointestinal dysfunction, respiratory suppression than morphine. Here we report discovery TRV130...
An electrochemical sensor for detection of organophosphate (OP) pesticides and nerve agents using zirconia (ZrO2) nanoparticles as selective sorbents is presented. Zirconia were electrodynamically deposited onto the polycrystalline gold electrode by cyclic voltammetry. Because strong affinity phosphoric group, nitroaromatic OPs strongly bind to ZrO2 nanoparticle surface. The characterization anodic stripping voltammetric performance bound evaluated square-wave (SWV) analysis. SWV was used...
In this article, we describe a disposable nucleic acid biosensor (DNAB) for low-cost and sensitive detection of samples in 15 min. Combining the unique optical properties gold nanoparticles (Au-NP) high efficiency chromatographic separation, sandwich-type DNA hybridization reactions were realized on lateral flow strips, which avoid multiple incubation, washing steps conventional biosensors. The captured Au-NP probes test zone control produced characteristic red bands, enabling visual without...
We report an aptamer−nanoparticle strip biosensor (ANSB) for the rapid, specific, sensitive, and low-cost detection of circulating cancer cells. Known their high specificity affinity, aptamers were first selected from live cells by cell-SELEX (systematic evolution ligands exponential enrichment) process. When next combined with unique optical properties gold nanoparticles (Au-NPs), ANSBs prepared on a lateral flow device. Ramos used as model target cell to demonstrate proof principle. Under...
The highly specific molecular recognition properties of aptamers are combined with the unique optical gold nanoparticles for development a dry-reagent strip biosensor that enables qualitative (visual)/quantitative detection protein within minutes. A model system comprising thrombin as an analyte and pair aptamer probes is used to demonstrate proof-of-concept on conventional lateral flow test strip. assay avoids multiple incubation washing steps performed in most current aptamer-based...
Band Jahn–Teller type structural instabilities of cubic Mn2YZ Heusler compounds causing tetragonal distortions can be predicted by ab initio band-structure calculations. This allows for identification new materials with tunable magnetic and properties that satisfy the demands spintronic applications, such as in spin-transfer torque-based devices. represent a remarkable class more than 1500 members. While they have been known long time,1 it is only recent years wide range their extraordinary...
We present a lateral flow aptasensor for the visual detection of alpha-fetoprotein (AFP) in human serum. Leveraging precise molecular recognition capabilities aptamers and distinct optical features gold nanoparticles, model system utilizing AFP as target analyte, along with pair aptamer probes, is implemented to establish proof-of-concept on standard test strips. It first report an antibody-free assay using probes AFP. The analysis circumvents numerous incubation washing steps that are...
An electrochemical immunoassay protocol for the simultaneous measurements of proteins, based on use different inorganic nanocrystal tracers is described. The multiprotein electrical detection capability coupled to amplification feature stripping transduction (to yield fmol limits) and with an efficient magnetic separation minimize nonspecific adsorption effects). multianalyte sandwich involves a dual binding event, antibodies linked tags beads. Carbamate linkage used conjugating...
We describe a disposable electrochemical immunosensor diagnosis device that integrates the immunochromatographic strip technique with an immunoassay and exploits quantum dot (QD, CdS@ZnS) as labels for amplifying signal output. The takes advantage of speed low cost conventional test high sensitivity nanoparticle-based immunoassay. A sandwich immunoreaction was performed on strip, captured QD in zone were determined by highly sensitive stripping voltammetric measurement dissolved metallic...
Signal amplification using enzyme multilayers on carbon nanotube (CNT) templates is shown to yield a remarkably sensitive electrochemical detection of proteins and nucleic acids. The electrostatic layer-by-layer (LBL) self-assembly onto CNT carriers maximizes the ratio tags per binding event offer greatest factor reported date. Absorption spectroscopy, TEM, characterization confirm formation LBL nanostructures individual carriers. enzymatic activity found increase with number layers. new...
A novel electrochemical immunosensor for the detection of tumor necrosis factor-alpha (TNF-α) based on poly(guanine)-functionalized silica nanoparticles (NPs) label is presented. The mouse TNF-α via immunological reaction a dual signal amplification: (1) large amount guanine residues introduced electrode surface through sandwich immunoreaction and NP label; (2) Ru(bpy)32+-induced catalytic oxidation guanine, which results in great enhancement anodic current. synthesized conjugates were...
A nanoparticle-based electrochemical immunosensor has been developed for the detection of phosphorylated acetylcholinesterase (AChE), which is a potential biomarker exposure to organophosphate (OP) pesticides and chemical warfare nerve agents. Zirconia nanoparticles (ZrO(2) NPs) were used as selective sorbents capture AChE adduct, quantum dots (ZnS@CdS, QDs) tags label monoclonal anti-AChE antibody quantify immunorecognition events. The sandwich-like immunoreactions performed among ZrO(2)...
We report a rapid and highly sensitive approach based on gold-nanoparticle-decorated silica nanorods (GNP-SiNRs) label lateral-flow strip biosensor (LFSB) for visually detecting proteins. Owing to its biocompatibility convenient surface modification, SiNRs were used as carriers load numerous GNPs, the GNP-SiNRs labels assay. The LFSB detection limit was lowered 50 times compared traditional GNP-based Rabbit IgG model target demonstrate proof-of-concept. Sandwich-type immunoreactions...
Biocatalysis of large-sized substrates finds wide applications. Immobilizing the involved enzymes on solid supports improves biocatalysis yet faces challenges such as enzyme structural perturbation, leaching, and low cost-efficiencies, depending immobilization strategies/matrices. Carbon nanotubes (CNTs) are attractive matrices but challenged by leaching (physical adsorption) or perturbation (covalent linking). Zeolitic imidazolate frameworks (ZIFs) overcome these issues. However, our recent...