A5100443019- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Tuberous Sclerosis Complex Research
- Microplastics and Plastic Pollution
- Animal Virus Infections Studies
- CAR-T cell therapy research
- Head and Neck Cancer Studies
- Viral Infections and Immunology Research
- Long-Term Effects of COVID-19
- Hepatitis B Virus Studies
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Gut microbiota and health
- Telomeres, Telomerase, and Senescence
- COVID-19 epidemiological studies
- Vaccine Coverage and Hesitancy
- Salivary Gland Disorders and Functions
- Recycling and Waste Management Techniques
- Mycobacterium research and diagnosis
- Asthma and respiratory diseases
- Bacterial Infections and Vaccines
Ministry of Agriculture and Rural Affairs
2020-2025
Kunming University of Science and Technology
2023-2024
First Affiliated Hospital of Xi'an Jiaotong University
2020-2024
Academy of Military Medical Sciences
2018-2024
Wuhan Institute of Virology
2017-2024
Chinese Academy of Sciences
2017-2024
Westlake University
2021-2024
Fudan University Shanghai Cancer Center
2024
Shanghai Medical College of Fudan University
2024
Beijing Fengtai Hospital
2022-2023
Developing therapeutics against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) could be guided by the distribution of epitopes, not only on receptor binding domain (RBD) Spike (S) protein but also across full protein. We isolated and characterized monoclonal antibodies (mAbs) from 10 convalescent COVID-19 patients. Three mAbs showed neutralizing activities authentic SARS-CoV-2. One mAb, named 4A8, exhibits high neutralization potency both pseudotyped SARS-CoV-2 does bind RBD....
This is the first randomised controlled trial for assessment of immunogenicity and safety a candidate non-replicating adenovirus type-5 (Ad5)-vectored COVID-19 vaccine, aiming to determine an appropriate dose vaccine efficacy study.This randomised, double-blind, placebo-controlled, phase 2 Ad5-vectored was done in single centre Wuhan, China. Healthy adults aged 18 years or older, who were HIV-negative previous severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection-free,...
Abstract The unprecedented coronavirus disease 2019 (COVID-19) epidemic has created a worldwide public health emergency, and there is an urgent need to develop effective vaccine control this severe infectious disease. Here, we find that single vaccination with replication-defective human type 5 adenovirus encoding the SARS-CoV-2 spike protein (Ad5-nCoV) protect mice completely against mouse-adapted infection in upper lower respiratory tracts. Additionally, Ad5-nCoV protects ferrets from...
SARS-CoV-2 has caused millions of deaths, and, since Aug 11, 2020, 20 intramuscular COVID-19 vaccines have been approved for use. We aimed to evaluate the safety and immunogenicity an aerosolised adenovirus type-5 vector-based vaccine (Ad5-nCoV) in adults without from China.This was a randomised, single-centre, open-label, phase 1 trial done Zhongnan Hospital (Wuhan, China), Ad5-nCoV by aerosol inhalation (≥18 years) seronegative SARS-CoV-2. Breastfeeding or pregnant women people with major...
Abstract Evidence for microbial degradation of polyvinyl chloride (PVC) has previously been reported, but little is known about the degrading strains and enzymes. Here, we isolate a PVC-degrading bacterium from gut insect larvae shed light on PVC pathway using multi-omic approach. We show that an pest, Spodoptera frugiperda , can survive by feeding film, this associated with enrichment Enterococcus Klebsiella other bacteria in larva’s microbiota. A bacterial strain isolated larval intestine...
Abstract Nipah virus (NiV) is a World Health Organization priority pathogen and there are currently no approved drugs for clinical immunotherapy. Through the use of naïve human phage-displayed Fab library, two neutralizing antibodies (NiV41 NiV42) targeting NiV receptor binding protein (RBP) were identified. Following affinity maturation, derived from NiV41 display cross-reactivity against both Hendra (HeV), whereas antibody based on NiV42 only specific to NiV. Results immunogenetic analysis...
Abstract The pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) presents a global public health threat. Most research on therapeutics against SARS-CoV-2 focused the receptor binding domain (RBD) Spike (S) protein, whereas vulnerable epitopes and functional mechanism non-RBD regions are poorly understood. Here we isolated characterized monoclonal antibodies (mAbs) derived from convalescent COVID-19 patients. An mAb targeting N-terminal...
Abstract Inspired by the timely emergence of silkworm pupae from their cocoons, chrysalis‐like probiotic composites (SCPCs) are developed for comprehensive therapy inflammatory bowel disease (IBD), in which probiotics enveloped as “pupa” a sequential layering silk sericin (SS), tannic acid (TA), and polydopamine, akin to protective “cocoon”. Compared unwrapped probiotics, these not only demonstrate exceptional resistance harsh gastrointestinal environment exhibit over 200 times greater...
Respiratory oscillometry is a promising complement to the traditional pulmonary function tests for its simplicity. The usefulness of in adult clinical practice has not been clarified. This study aimed analyse characteristics and diagnostic performance respiratory diseases, explore cut-offs oscillometric parameters severity grading.In this multicentre registry impulse (IOS), IOS spirometric data healthy individuals patients with diseases were collected analysed. Linear mixed model analysis...
Abstract The SARS-CoV-2 Omicron variant shows substantial resistance to neutralization by infection- and vaccination-induced antibodies, highlighting the demands for research on continuing discovery of broadly neutralizing antibodies (bnAbs). Here, we developed a panel bnAbs against other variants concern (VOCs) elicited vaccination adenovirus-vectored COVID-19 vaccine (Ad5-nCoV). We also investigated human longitudinal antibody responses following demonstrated how evolved over time. A...
Coinfection of human immunodeficiency virus type 1 (HIV-1) is the most significant risk factor for tuberculosis (TB). The immune responses lung are essential to restrict growth Mycobacterium and avoid emergence disease. Nevertheless, there still limited knowledge about local response in people with HIV-1-TB coinfection.
Lampreys are early jawless vertebrates that the key to understanding evolution of vertebrates. However, lack cytomic studies on multiple lamprey organs has hindered progress in this field. Therefore, present study constructed a comprehensive cell atlas comprising 604,460 cells/nuclei and 70 types from 14 tissue samples. Comparison cellular across species revealed most homologous those jawed We discovered acinar- islet-like populations despite parenchymal lampreys, providing evidence...
Abstract Human papillomavirus (HPV) is responsible for increasing incidence of oropharyngeal cancer. At present, there are no biomarkers in the surveillance algorithm HPV-positive cancer (HPV-OPC). HPV16 E6 antibody precedes diagnosis. If indeed primary diagnosis, it similarly expected to precede disease recurrence and may have a potential role as biomarker HPV-OPC. To determine whether HPV titers early markers or prognosis, retrospective pilot study was designed E6, E7, E1, E2 decrease...
The Middle East respiratory syndrome coronavirus (MERS-CoV) was first isolated in 2012, and circulated worldwide with high mortality. continual outbreaks of MERS-CoV highlight the importance developing antiviral therapeutics. Here, we rationally designed a novel fusion inhibitor named MERS-five-helix bundle (MERS-5HB) derived from six-helix (MERS-6HB) which formed by process membrane fusion. MERS-5HB consists three copies heptad repeat 1 (HR1) two 2 (HR2) while MERS-6HB includes each HR1...
A licensed vaccine against Ebola virus (EBOV) remains unavailable, despite >11 000 deaths from the 2014–2016 outbreak of EBOV disease in West Africa. Past studies have shown that recombinant viruses expressing glycoprotein (GP) are able to protect nonhuman primates (NHPs) a lethal challenge. However, these vaccines express viral GP–based variants found Central Africa, which has 97.3% amino acid homology Makona variant Our previous study showed adenovirus serotype 5 (Ad5)–vectored GP (MakGP)...
Background: To determine the safety and immunogenicity of a novel recombinant adenovirus type 5 vector based Ebola virus disease vaccine (Ad5-EBOV) in Africans China.Methods: A phase 1, dose-escalation, open-label trial was conducted. 61 healthy were sequentially enrolled, with 31 participants receiving one shot intramuscular injection 30 double-shot regimen. Primary secondary end points related to assessed within 28 d after vaccination. This study registered ClinicalTrials.gov...
The proper route for vaccine delivery plays an important role in activating a robust immune response. Several viral vector-based vaccines against Ebola disease administered intramuscularly have been found to excellent immunogenicity and protectiveness. In this study, we evaluated different routes Ad5-EBOV by comparing humoral cellular responses, germinal center reactions, dendritic cell activation antigen expression. Mice injected with the exhibited advantage expression, leading more while...
Abstract The SARS-CoV-2 Omicron variant has become the dominant around world and exhibits immune escape to current COVID-19 vaccines some extent due its numerous spike mutations. Here, we evaluated responses booster vaccination with intramuscular adenovirus-vectored vaccine (Ad5-nCoV), aerosolized Ad5-nCoV, a recombinant protein subunit (ZF2001) or homologous inactivated (CoronaVac) in those who received two doses of 6 months prior. We found that Ad5-nCoV induced potent neutralizing activity...