A5100419292- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- HIV/AIDS Research and Interventions
- Virus-based gene therapy research
- HIV Research and Treatment
- Tuberculosis Research and Epidemiology
- COVID-19 Clinical Research Studies
- HIV/AIDS drug development and treatment
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Amoebic Infections and Treatments
- Viral gastroenteritis research and epidemiology
- HIV, Drug Use, Sexual Risk
- Parasites and Host Interactions
- Parasitic Diseases Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Vector-Borne Animal Diseases
- Diagnosis and treatment of tuberculosis
- COVID-19 epidemiological studies
- SARS-CoV-2 detection and testing
- Energy and Environment Impacts
- Biochemical and Molecular Research
- Animal Virus Infections Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Wenzhou City People's Hospital
2024
National Vaccine and Serum Institute
2022-2023
Fudan University
2023
Zhongshan Hospital
2023
The People's Hospital of Guangxi Zhuang Autonomous Region
2023
Beijing Fengtai Hospital
2022
Shanghai Ninth People's Hospital
2022
Shanghai Jiao Tong University
2020-2022
Sichuan University
2022
Academy of Military Medical Sciences
2022
Abstract The unprecedented coronavirus disease 2019 (COVID-19) epidemic has created a worldwide public health emergency, and there is an urgent need to develop effective vaccine control this severe infectious disease. Here, we find that single vaccination with replication-defective human type 5 adenovirus encoding the SARS-CoV-2 spike protein (Ad5-nCoV) protect mice completely against mouse-adapted infection in upper lower respiratory tracts. Additionally, Ad5-nCoV protects ferrets from...
Abstract Background To evaluate the prognostic value of pre-treatment aspartate transaminase (AST)/alanine (ALT) ratio in hepatocellular carcinoma (HCC) patients receiving radiofrequency ablation (RFA)/microwave (MWA) combined with simultaneous TACE. Methods The data for 117 were retrospectively analyzed this study. endpoint prognosis was overall survival (OS). Youden index used to choose optimal cut-off AST/ALT OS prediction. Univariate and multivariate analyses identify independent risk...
The proper route for vaccine delivery plays an important role in activating a robust immune response. Several viral vector-based vaccines against Ebola disease administered intramuscularly have been found to excellent immunogenicity and protectiveness. In this study, we evaluated different routes Ad5-EBOV by comparing humoral cellular responses, germinal center reactions, dendritic cell activation antigen expression. Mice injected with the exhibited advantage expression, leading more while...
T-cell immunity plays an important role in the control of SARS-CoV-2 and has a great cross-protective effect on variants. The Omicron BA.1 variant contains more than 30 mutations spike severely evades humoral immunity. To understand how affect cellular immunity, epitopes wild-type BALB/c (H-2d) C57BL/6 mice (H-2b) were mapped through IFNγ ELISpot intracellular cytokine staining assays. identified verified splenocytes from vaccinated with adenovirus type 5 vector encoding homologous spike,...
Early antiretroviral therapy (ART) initiation is a recommended public health approach for the prevention of HIV-1 transmission. In this cohort study, we included 13132 serodiscordant couples. ART was initiated patients with CD4+ T cell counts less than 200 cells/uL, 350 and 500 cells/uL respectively. This divided treated couples into three groups. Univariate multivariate intention-to-treat analyses were performed to examine association between study Early-ART associated 45% lower risk...
Rift Valley fever virus (RVFV) is listed as a priority pathogen by the World Health Organization (WHO) because it causes serious and fatal disease in humans, there are currently no effective countermeasures. Therefore, urgent to develop safe efficacious vaccine. Here, we developed six nucleotide-modified mRNA vaccines encoding different regions of Gn Gc proteins RVFV encapsulated lipid nanoparticles, compared their ability induce immune responses mice found that vaccine full-length had...
Trichinellosis is a serious food-borne parasitic zoonosis worldwide. In the effort to develop vaccine against Trichinella infection, we have identified spiralis Heat shock protein 70 (Ts-Hsp70) elicits partial protective immunity T. infection via activating dendritic cells (DCs) in our previous study. This study aims investigate whether DCs were activated by Ts-Hsp70 through TLR2 and/or TLR4 pathways.After blocking with anti-TLR2 and antibodies, binding of was significantly reduced. The...
Helminths develop strategies to escape host immune responses that facilitate their survival in the hostile environment. Trichinella spiralis, a tissue-dwelling nematode, has developed sophisticated strategy complement attack. Our previous study demonstrated T. spiralis secretes calreticulin (TsCRT) inhibit classical activation through binding C1q; however, C1q site TsCRT and specific mechanism involved with complement-related evasion remains unknown. Using molecular docking modeling fragment...
Adenovirus type 7 (HAdV7) is one of the most pathogenic human adenoviruses (HAdVs) and can cause severe illness even death, particularly in people with weakened immune systems. Many countries worldwide have experienced epidemics this highly contagious pathogen, including China Sierra Leone; however, studies describing seroprevalence anti-HAdV7 neutralizing antibodies (nAbs) are still lacking. Herein, we established an efficient neutralization assay based on a recombinant...
Abstract Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world, updated vaccines innovative vaccination strategies are urgently needed to address global SARS-COV2 pandemic. Here, we established a series of Ad5-vectored variant encoding multiple spike proteins derived from Alpha, Beta, Gamma, Epsilon, Kappa, Delta Omicron lineages analyzed antibody immune responses induced by single-dose prime-boost against emerging concern (VOCs). Single-dose with tended...
Instruction: Rift valley fever virus (RVFV) is a mosquito-transmitted bunyavirus that causes severe disease in animals and humans. Nevertheless, there are no vaccines applied to prevent RVFV infection for human at present. Therefore, it necessary develop safe effective vaccine. Methods: We generated Ad5-GnGcopt, replication-deficient recombinant Ad5 vector (human adenovirus serotype 5) expressing codon-optimized glycoproteins Gn Gc, evaluated its immunogenicity protective efficacy mice....
Rift Valley fever virus (RVFV) is one of the most important virulent pathogens causing severe disease in animals and humans. However, there currently no approved vaccine to prevent RVFV infection The use human adenovirus serotype 4 (Ad4) as a vector for an has not been reported. Here, we report generation replication-competent recombinant Ad4 expressing codon-optimized forms glycoproteins Gn Gc (named Ad4-GnGc). Intramuscular immunization with Ad4-GnGc elicited robust neutralizing antibodies...
The prevalence of pyogenic liver abscess (PLA) is increasing worldwide. However, evaluation on its economic burden still lack. A retrospective study that included all patients identified PLA from 2017 to 2020 was conducted. Clinical information and hospital costs were collected through the electronic medical records. We evaluated using disability-adjusted life years (DALYs). Differences in socioeconomic burdens between Klebsiella pneumoniae-caused abscesses (KPLA) non-Klebsiella (non-KPLA)...
In this study, the Mycobacterium tuberculosis (MTB) latency-associated antigens Rv2660c, Rv1733c, Rv1813c, Rv2628, Rv2029c, and Rv2659c were compared regarding their immunogenicity potential therapeutic effects in an MTB reactivation mouse model. Normal mice or immunized intramuscularly three times at 2-week intervals with saline, plasmid vector pVAX1, vaccae vaccine (a commercial inactivated vaccine), rv1813c DNA, rv2628 rv2029c rv2659c rv1733c rv2660c DNA. The normal DNA had low numbers of...
Abstract Objectives To evaluate the prevention efficacy of scaling up HIV/AIDS antiretroviral therapy (ART) on HIV transmission at population level and determine associated factors secondary transmission. Methods We used longitudinal molecular networks to assess genetic linkage between baseline newly diagnosed cases. A generalized estimating equation was applied associations demographic, clinical characteristics Results Patients ART had a 32% lower risk than those not ART. 36% reduction in...
Abstract HIV with HBV co-infection can result in greater HIV-related immunosuppression, morbidity and mortality. Currently, there are few studies to evaluate direct treatment effects on mortality attrition rates between first-line antiretroviral therapy (ART) based-on tenofovir (TDF) and/or lamivudine (3TC) a real-world setting. We used Cox proportional hazard models of the ART containing stavudine (d4T), azidothymidine (AZT) TDF death among patients coinfection. A total 3912 met study...
<title>Abstract</title> Background Novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) is pandemic. However, data concerning the epidemiological features, viral shedding, and antibody dynamics between asymptomatic SARS-CoV-2 carriers COVID-19 patients remain controversial. Methods A total of 193 subjects in Ningbo Zhoushan, Zhejiang, China, were enrolled this study from January 21 to March 6, 2020. All tested positive for genomic RNA...
Abstract Amino acid thiophosphoramidates of nucleoside were synthesized and determined by negative ion electrospray ionization mass spectrometry (ESI‐MS) in conjunction with tandem (MS/MS). Hie fragmentation pathways investigated. The results show that ESI‐MS is a useful tool for structural determination amino nucleoside.
Abstract At present, several countries, such as Germany and India, have employed a pool testing method on the nucleic acid of COVID-19 for shortage detection kits. In this method, is performed samples cases together bunch. If test result bunch negative, then it shown that none in has been infected with novel coronavirus. On contrary, if positive, are tested one by to confirm which infected. We verified effective situation kits based probabilistic modeling. Moreover, following interesting...
Designing a modified virus that can be controlled to replicate will facilitate the study of pathogenic mechanisms and virus-host interactions. Here, we report universal switch element enables precise control replication after exposure small molecule. Inteins mediate traceless protein splicing-ligation process, generate series vesicular stomatitis (VSV) with intein insertion into nucleocapsid, phosphoprotein, or large RNA-dependent RNA polymerase VSV. Two recombinant VSV, LC599 LY1744, were...