A5047233935- Parasitic Diseases Research and Treatment
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Complement system in diseases
- Antifungal resistance and susceptibility
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Immune Response and Inflammation
- Xenotransplantation and immune response
- Advanced Glycation End Products research
- Rheumatoid Arthritis Research and Therapies
- Bacterial Infections and Vaccines
- Insects and Parasite Interactions
- Transgenic Plants and Applications
- Systemic Lupus Erythematosus Research
- Nanoplatforms for cancer theranostics
- Cytomegalovirus and herpesvirus research
- Ocular Surface and Contact Lens
Capital Medical University
2011-2024
Soochow University
2006-2023
George Washington University
2023
Zunyi Medical University
2014
Capital University
2009
First Affiliated Hospital Zhejiang University
1999
Trichinellosis is a worldwide zoonosis and remains serious public health problem. Interrupting parasite transmission via vaccination of livestocks with potent vaccine practical approach to prevent human Trichinellosis. Our previous studies have identified that paramyosin Trichinella spiralis (Ts-Pmy) good candidate against In this study, novel multi-epitope (MEP) was constructed by using four CD4+ T cell epitopes (P2, P3, P4, P5) one B epitope (YX1) from Ts-Pmy expressed as soluble...
Background Many evidences show the inverse correlation between helminth infection and allergic or autoimmune diseases. Identification characterization of active helminth-derived products responsible for beneficial effects on inflammatory diseases will provide another feasible approach to treat these Methods Findings Colitis was induced in C57BL/6 mice by giving 3% DSS orally 7 days. During this period, were treated daily with excretory/secretory from T. spiralis adult worms (AES)...
The hygiene hypothesis proposes that decreased exposure to infectious agents in developed countries may contribute the development of allergic and autoimmune diseases. Trichinella spiralis, a parasitic roundworm, causes trichinellosis, also known as trichinosis, humans. T. spiralis had many hosts, almost any mammal could become infected. Adult worms lived small intestine, while larvae muscle cells same mammal. was significant public health threat because it cause severe illness even death...
Trichinellosis, a widespread zoonosis, is regarded as an emerging or reemerging disease. Effective treatment and prognosis of trichinellosis depends on early diagnosis the infection. The objective this study was to identify sensitive specific antigens for effective vaccine preventing somatic proteins T. spiralis adult worms were separated by two-dimensional gel electrophoresis (2-DE). probed with infection sera from swine mice infected 7 days. primary immunoreactive spots characterized...
Background Our previous studies showed that Trichinella spiralis paramyosin (TsPmy) is an immunomodulatory protein inhibits complement C1q and C8/C9 to evade host attack. Vaccination with recombinant TsPmy induced protective immunity against T. larval challenge. Due the difficulty in producing as a soluble protein, we prepared DNA vaccine alternative approach order elicit robust infection. Methods Findings The full-length coding was cloned into eukaryotic expression plasmid pVAX1,...
The easy recurrence and high metastasis of fatal tumors require the development a combination therapy, which is able to overcome drawbacks monomodal strategies such as surgery, photodynamic therapy (PDT), radiotherapy (RT). Taking complementary advantages PDT RT, we present herein integration lanthanide-doped upconversion nanoparticles (UCNPs) with chlorin e6 (Ce6)-imbedded RBC membrane vesicles near-infrared-induced agent for achieving synchronous depth RT reduced radiation exposure. In...
Paramyosin is a thick myofibrillar protein found exclusively in invertebrates. Evidence suggested that paramyosin from helminths serves not only as structural but also an immunomodulatory agent. We previously reported recombinant Trichinella spiralis (Ts-Pmy) elicited partial protective immunity mice. In this study, the ability of Ts-Pmy to bind host complement components and protect against attack was investigated.In transcriptional expression levels were determined T. newborn larva (NBL),...
Trichinellosis is one of the most important food-borne parasitic zoonoses throughout world. Because infected pigs are major source human infections, and China becoming largest international producer pork, development a transmission-blocking vaccine to prevent swine from being urgently needed for trichinellosis control in China. Our previous studies have demonstrated that specific Trichinella spiralis paramyosin (Ts-Pmy) Ts-87 antigen could provide protective immunity against T. infection...
Trichinella spiralis heat shock protein 70 (Ts-Hsp70) is a protective antigen that induces partial immunity against T. infection in mice. To determine whether dendritic cells are involved the mechanism responsible for protection induced by Ts-Hsp70, mouse bone marrow-derived (DCs) were incubated with recombinant Ts-Hsp70 (rTs-Hsp70), and DC-secreted cytokines expressed surface markers measured. The results demonstrated rTs-Hsp70 activated DC maturation was characterized secretion of IL-1β,...
Trichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired during is important for vaccine development and immunodiagnosis.The T. adult cDNA library was immunoscreened with sera from pigs experimentally infected 20,000 infective larvae. Total 43 positive clones encoding 28 proteins were identified; one immunodominant 20 kDa Ts-ES-1 secreted by stichocytes existing excretory/secretory (ES) products muscle...
Trichinellosis is a serious food-borne parasitic zoonosis worldwide. In the effort to develop vaccine against Trichinella infection, we have identified spiralis Heat shock protein 70 (Ts-Hsp70) elicits partial protective immunity T. infection via activating dendritic cells (DCs) in our previous study. This study aims investigate whether DCs were activated by Ts-Hsp70 through TLR2 and/or TLR4 pathways.After blocking with anti-TLR2 and antibodies, binding of was significantly reduced. The...
Dendritic cells (DCs) are antigen-presenting that regulate T cell responses for many infectious diseases. The tissue-dwelling nematode Trichinella spiralis expresses paramyosin (TsPmy) not only as a structural protein but also an immunomodulator to alleviate complement attack by binding some host components. Whether TsPmy is involved in other immunomodulatory pathway and how interacts with DCs still unknown. Mouse bone marrow-derived were incubated recombinant (rTsPmy) activation. Maturation...
Trichinellosis is an important foodborne zoonosis that distributed worldwide. Trichinella spiralis may evade host complement-mediated attack by expressing complement inhibitory proteins, such as paramyosin (Pmy). Previous studies have shown (Ts-Pmy) able to bind the human component C9 inhibit activation and protect parasite from attack. Further determination of complement-binding domain on Ts-pmy will enable us better understand Ts-Pmy's biofunction in immune evasion provide feasible...
Multiepitope peptide vaccine has some advantages over traditional recombinant protein due to its easy and fast production possible inclusion of multiple protective epitopes pathogens. However, it is usually poorly immunogenic needs conjugate a large carrier protein. Peptides conjugated central lysine core form antigen peptides (MAPs) will increase the immunogenicity vaccine. In this study, we constructed MAP consisting CD4 + T cell B paramyosin (Pmy) Trichinella spiralis ( Ts -Pmy), which...
Blockade of the interactions between CD28/CTLA-4 and their ligands, CD80 (B7, B7.1)/CD86 (B70, B7.2), is an attractive means to induce antigen-specific peripheral tolerance in autoimmune disease organ transplantation. In this study, we generated characterized a monoclonal antibody (Clone 4E5) against human CD80. 4E5 could recognize both mouse suppress mixed lymphocyte reaction vitro. To investigate potency for clinical use, further administrated lupus-like model (C57BL/J6) induced by...
Integrin-linked kinase (ILK) is an integrin-binding cytoplasmic protein that has been implicated in regulating numerous cellular processes and fibronectin (Fn) deposition through mediated integrin, but the expression significance of ILK aging kidney have not yet reported. We report here mRNA increased primary cultured mesangial tubular epithelial cells, normal unilateral ureteral obstructed tissues 28-month-old rats 3-month-old rats, moreover, accompanied by over-expression Fn integrin-β1...
Trichinella spiralis expresses paramyosin (Ts-Pmy) not only as a structural protein but also an immunomodulator that inhibits host complement survival strategy. Previous studies demonstrated Ts-Pmy bound to components C8 and C9 inhibited the polymerization of during formation membrane attack complex (MAC). The binding domain was identified within 14 amino acid residues at C-terminus Ts-Pmy. production monoclonal antibody specifically targets site is necessary for further function may be used...
Trichinellosis is a widespread zoonosis primarily caused by Trichinella spiralis . Mucosal immunity crucial for preventing infection. In our previous study, DNA vaccine with the antigen Ts87 delivered an attenuated Salmonella typhimurium elicited partial protection against infection in mice. current to elicit more robust immune response and develop potent vaccination strategy trichinellosis, heterologous prime-boost regimen was used mice protective efficacy evaluated compared homologous or...
Trichinellosis is a serious food-borne parasitic zoonosis worldwide. Different host macrophage subsets play various roles during helminth infection; however, the dynamic changes in following Trichinella spiralis infection have not been reported. Here, flow cytometry and immunofluorescence were used to assess activation mesenteric lymph nodes (MLN), spleen, intestine, muscle from T. spiralis-infected mice at 1, 5, 15, 30 days post (dpi). Macrophages MLN, spleen tended be activated M1-type 1 5...