Saeed El‐Ashram

ORCID: 0000-0003-0389-1980
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About
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Research Areas
  • Toxoplasma gondii Research Studies
  • Coccidia and coccidiosis research
  • Animal Nutrition and Physiology
  • Herpesvirus Infections and Treatments
  • Parasitic Infections and Diagnostics
  • Helminth infection and control
  • Viral gastroenteritis research and epidemiology
  • Meat and Animal Product Quality
  • Rabbits: Nutrition, Reproduction, Health
  • Animal Virus Infections Studies
  • Cytomegalovirus and herpesvirus research
  • Rabies epidemiology and control
  • Environmental Toxicology and Ecotoxicology
  • Salmonella and Campylobacter epidemiology
  • Insect and Pesticide Research
  • Nanoparticles: synthesis and applications
  • Viral Infections and Vectors
  • Vector-borne infectious diseases
  • Microbial infections and disease research
  • Trypanosoma species research and implications
  • Parasites and Host Interactions
  • Parasite Biology and Host Interactions
  • Aquaculture disease management and microbiota
  • interferon and immune responses
  • Probiotics and Fermented Foods

Foshan University
2017-2025

Kafrelsheikh University
2016-2025

Istanbul Medipol University
2025

Ankara Medipol Üniversitesi
2025

University of Arkansas at Fayetteville
2021-2023

King Saud University
2023

Van Yüzüncü Yıl Üniversitesi
2023

Life University
2020

China Agricultural University
2011-2018

University of Guelph
2015

Farmed fish and shrimp are continuously challenged by multiple stressors during their life stages, such as hypoxia, pH fluctuations, different salinities, high nitrite, un-ionized ammonia, injury handling, inadequate nutrition, or food shortage, which can eventually adversely impact health, welfare, growth rates. Besides, these weaken production decrease resistance to diseases. Scientists researchers have been making concerted efforts find new, safe, inexpensive supplements mitigate the...

10.1016/j.aqrep.2022.101135 article EN cc-by Aquaculture Reports 2022-05-09

Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, toxic material free protocol extracting DNA RNA from different prokaryotic eukaryotic sources exploiting the physical chemical properties of acids. Furthermore, this showed that under triple protection (i.e. EDTA, SDS NaCl) lysis step, environment is improper RNase to have liberated even DNase degrade DNA. Therefore, complete removal...

10.1016/j.btre.2016.10.001 article EN cc-by Biotechnology Reports 2016-10-09

The hygiene hypothesis proposes that decreased exposure to infectious agents in developed countries may contribute the development of allergic and autoimmune diseases. Trichinella spiralis, a parasitic roundworm, causes trichinellosis, also known as trichinosis, humans. T. spiralis had many hosts, almost any mammal could become infected. Adult worms lived small intestine, while larvae muscle cells same mammal. was significant public health threat because it cause severe illness even death...

10.1371/journal.ppat.1011893 article EN cc-by PLoS Pathogens 2024-01-02

Abstract Clustering is an unsupervised approach to classify elements based on their similarity, and it used find the intrinsic patterns of data. There are enormous applications clustering in bioinformatics, pattern recognition, astronomy. This paper presents a idea that density wise single or multiple connected regions make cluster, which maxima point represents center corresponding region. More precisely, our firstly finds local subsequently merges form meaningful clusters. empowers...

10.1038/srep45602 article EN cc-by Scientific Reports 2017-04-19

Mesenchymal stem cells (MSCs) are the most promising seed for cell therapy. Comparing biological and transcriptome gene characteristics of MSCs from different sources provides an important basis screening clinically used cells. The main purpose this experiment was to establish methods isolation culture five canine sources, including adipose tissue, bone marrow, umbilical cord, amniotic membrane, placenta, compare MSCs, in order provide a clinical application MSCs. were isolated Chinese...

10.3390/ijms20061485 article EN International Journal of Molecular Sciences 2019-03-25

Porcine epidemic diarrhea virus ( PEDV ), a leading cause of piglet outbreaks, poses significant danger to the swine industry. The aim this study was investigate characteristics that circulating in Guangdong province, one China’s major pig producing provinces. Clinical samples were collected from eight farms province between 2018 and 2019 tested for porcine enteric pathogens, including , transmissible gastroenteritis TGEV) Swine coronavirus SeCoV) acute syndrome SADS-CoV) deltacoronavirus...

10.1371/journal.pone.0253622 article EN cc-by PLoS ONE 2021-06-24

Abstract With the evolution of nanostructure materials, silver nanoparticles (Ag-NPs) emerged as predominantly exploited nanomaterial in multifarious sectors due to their versatile properties. Along with heightening applications Ag-NPs, however, there is increasing concern over indubitable toxicity towards ecosystem, which indeed affects surrounding organisms and human health. In this study, we evaluated detrimental effects Ag-NPs relation Egyptian wild female beetles, Blaps polychresta ,...

10.1038/s41598-022-17712-z article EN cc-by Scientific Reports 2022-08-16

Toxoplasma gondii (T. gondii), a parasitic intracellular protozoan, can establish chronic infection in the host brain and cause significant neuropathology. The current study aimed to determine role of Tyrosine Hydroxylase (TH), Dopamine Receptor D1 (D1R), Nuclear Related-1 (Nurr1), Transporter (DAT) expression neuroimmunopathogenesis toxoplasmic encephalitis (TE) at 15, 30, 45, 60 days after with T. gondii. Additionally, investigated whether there was correlation between markers on these...

10.14670/hh-18-877 article EN PubMed 2025-01-23

Nanomedicine has emerged as a promising solution for treating various diseases. This study inspected the in vivo anticoccidial efficacy of biosynthesized zinc oxide nanoparticles (Bio-ZnNPs) from Coriandrum sativum leaves mice infected with Eimeria papillata. Bio-ZnNPs were described using transmission electron microscopy (TEM), UV-visible spectroscopy, and x-ray diffraction (XRD). Five groups applied: control group (uninfected), (uninfected, receiving 50 mg/kg Bio-ZnNPs), an infection...

10.1002/jemt.24839 article EN Microscopy Research and Technique 2025-03-07

Abstract Background Infection with the protozoan Toxoplasma gondii causes serious public health problems and is of great economic importance worldwide. Protection from acute toxoplasmosis known to be mediated by CD8+ T cells, but T. antigens host genes required for eliciting protective immunity have been poorly defined. The dense granule protein 6 (GRA6), recently proved highly immunogenic produces fully immune protection in infected BALB/c mice an H-2L d gene. cell response strain seemed...

10.1186/1756-3305-4-213 article EN cc-by Parasites & Vectors 2011-11-09

ABSTRACT Coccidiosis, caused by different species of Eimeria parasites, is an economically important disease poultry and livestock worldwide. Here we report previously unknown alterations in the gut microbes metabolism BALB/c mice infected with falciformis . Specifically, observed a significant shift abundance cecal bacteria disrupted parasitized animals. The relative abundances Lachnospiraceae bacterium NK4A136, Ruminiclostridium , Alistipes Lactobacillus declined response to E. infection,...

10.1128/iai.00073-18 article EN Infection and Immunity 2018-02-10

Abstract In this study, 140 cattle STEC isolates belonging to serogroups O157, O26, O145, O121, O103 and O45 were characterized for 38 virulence-associated genes, antimicrobial resistance profiles genotyped by PFGE. The majority of carried both stx1 stx 2 concurrently, stx2c , stx2d ; plasmid-encoded genes ehxA, espP, subA saa but lacked katP etpD eaeA . Possession was significantly associated with the presence nle ureC terC However, subA, stx1c stx1d only detected in negative isolates. A...

10.1038/s41598-019-47948-1 article EN cc-by Scientific Reports 2019-08-15

This study was conducted to investigate the causes of mortality in young rabbits. A total 110 V-Line breed female rabbits aged 5 m were used this study. Rabbit kits examined daily pre- and postweaning stages detect clinical disorders that caused death. The postmortem examination carried out on dead kits. Furthermore, for probable bacteriological parasitological Fecal samples collected from each kit by standard microbiological procedures bacterial pathogens macroscopically microscopically...

10.3390/ani10030537 article EN cc-by Animals 2020-03-24

The present study evaluated the feed additive containing essential oils of thyme and star anise from 1 to 42 D age on growth performance, physiological parameters, carcass traits, intestinal bacterial community, antioxidant status meat, economic efficiency in broilers under Egyptian weather condition. Two hundred seventy 1-day-old Indian River broiler chicks were randomly distributed three groups fed a basal diet for D. control (Co) group, T1 T2 group 0.0 mg/kg diet, 150 300 respectively....

10.1016/j.japr.2020.07.005 article EN cc-by-nc-nd The Journal of Applied Poultry Research 2020-08-21

Litter traits are critical economic variables in the pig industry as they represent a production indicator that can serve to determine sow fertility. In this study, genome-wide association study on litter traits, including total number born (TNB), alive (NBA), birth weight (LBW), average (ABW), and piglet uniformity (PU), was carried out two breeds (Yorkshire Landrace). A of 3 637 pigs both were genotyped using GeneSeek GGP Porcine 50K SNP BeadChip. mixed linear model (MLM) fixed random...

10.1016/j.animal.2022.100672 article EN cc-by-nc-nd animal 2022-10-28

High-throughput sequencing technology has shown tremendous promise for microbial community composition and diversity. Illumina MiSeq platform was exploited to study the associated with different stages of life-cycle ovine Haemonchus contortus field strains using two distinct amplification primer sets (targeting V3-V4, V5-V7). Scanning electron microscope polymerase chain reaction coupled were employed confirm absence any parasite surface contamination by intact bacteria or their DNA...

10.1038/s41598-017-00171-2 article EN cc-by Scientific Reports 2017-02-28

Cyclic GMP-AMP synthase (cGAS) is an important cytosolic DNA sensor that plays a crucial role in triggering STING-dependent signal and inducing type I interferons (IFNs). cGAS for intracellular bacterial recognition innate immune responses. However, the regulating effect of pathway bone marrow-derived dendritic cells (BMDCs) during Mycobacterium bovis (M. bovis) infection still unknown. We hypothesized maturation activation BMDCs were modulated by cGAS/STING/TBK1/IRF3 signaling pathway. In...

10.3390/ijms20040895 article EN International Journal of Molecular Sciences 2019-02-19
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