A5061182783- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Nerve injury and regeneration
- Antimicrobial Peptides and Activities
- Mycobacterium research and diagnosis
- Cancer Research and Treatments
- Mesenchymal stem cell research
- Pharmaceutical and Antibiotic Environmental Impacts
- Reproductive System and Pregnancy
- Enterobacteriaceae and Cronobacter Research
- Protein Hydrolysis and Bioactive Peptides
- Ocular Disorders and Treatments
- Bacterial biofilms and quorum sensing
- Neurogenesis and neuroplasticity mechanisms
- RNA regulation and disease
- Tissue Engineering and Regenerative Medicine
- Neuropeptides and Animal Physiology
- Trace Elements in Health
- Salmonella and Campylobacter epidemiology
- Sperm and Testicular Function
- Drug Solubulity and Delivery Systems
- Glaucoma and retinal disorders
- Veterinary medicine and infectious diseases
- Microbial infections and disease research
Foshan University
2015-2024
Sun Yat-sen University
2022-2024
University of Guelph
2018-2021
Huazhong Agricultural University
2012-2016
Altrenogest (ALT), a synthetic progestogen, serves critical role in estrus synchronization among animals like gilts and mares. However, its practical application animal husbandry is hampered due to poor solubility limited oral bioavailability. To address this challenge, solvent evaporation method was employed create an inclusion complex of ALT with hydroxypropyl-β-cyclodextrin (ALT/HP-β-CD). The formation confirmed by scanning electron microscopy, power X-ray diffraction, differential...
Strains of extraintestinal pathogenic Escherichia coli (ExPEC) can invade and colonize sites cause a wide range infections. Genomic analysis ExPEC has mainly focused on isolates human avian origins, with porcine yet to be sequenced. To better understand the genomic attributes underlying pathogenicity ExPEC, we isolated two E. strains PCN033 PCN061 from pigs, assessed their in vivo virulence, completed compared genomes. Animal experiments demonstrated that strain PCN033, but not PCN061, was...
Mesenchymal stem cells (MSCs) are the most promising seed for cell therapy. Comparing biological and transcriptome gene characteristics of MSCs from different sources provides an important basis screening clinically used cells. The main purpose this experiment was to establish methods isolation culture five canine sources, including adipose tissue, bone marrow, umbilical cord, amniotic membrane, placenta, compare MSCs, in order provide a clinical application MSCs. were isolated Chinese...
As part of the central nervous system, optic nerve, composed axons from retinal ganglion cells (RGCs), generally fails to regenerate on its own when injured in adult mammals. An innovative approach promoting nerve regeneration involves manipulating interactions between amacrine (ACs) and RGCs. Here, we identified a unique AC subtype, dopaminergic ACs (DACs), that responded early after crush by down-regulating neuronal activity reducing dopamine (DA) release. Activating DACs or augmenting DA...
The aim of this study was to assess the efficacy canine umbilical cord mesenchymal stem cells (UC-MSCs) on treatment knee osteoarthritis in dogs. Eight dogs were evenly assigned two groups. model established by surgical manipulation articular cartilage these eight UC-MSCs isolated from Wharton's jelly 0.1% type collagenase I and identified immunofluorescence staining adipogenic osteogenic differentiation vitro. A suspension allogeneic (1 × 106) an equal amount physiological saline injected...
Vision depends on accurate signal conduction from the retina to brain through optic nerve, an important part of central nervous system that consists bundles axons originating retinal ganglion cells. The mammalian system, cannot regenerate once it is injured, leading permanent vision loss. To date, there no clinical treatment can nerve and restore vision. Our previous study found mobile zinc (Zn2+) level increased rapidly after injury in retina, specifically vesicles inner plexiform layer....
Extraintestinal pathogenic Escherichia coli (ExPEC) is an important pathogen that can cause systemic infections in a broad spectrum of mammals and birds. To date, commercial vaccines against ExPEC pigs are rare antibiotic resistance has become serious clinical problem. Identification protective antigens helpful for developing potentially effective vaccines. In this study, two outer membrane porins, OmpC OmpF, porcine were cloned expressed to investigate their immunogenicity. Intraperitoneal...
This study aimed to identify tigecycline-resistant tet(X4)-bearing Escherichia fergusonii isolated from pigs in China with a complete genome sequence.E. was by selective medium and screened tigecycline-supplemented agar plates. The microbroth dilution method used for antimicrobial susceptibility testing, the minimal inhibitory concentration (MIC) results refer interpretation standard Clinical Laboratory Standards Institute of America (CLSI). Whole-genome sequencing performed on Illumina...
Retinal ganglion cells (RGCs), the projection neurons of eye, are irreversibly lost once optic nerve is injured, which a critical mechanism glaucoma. Mobile zinc (Zn2+) levels rapidly increase in retinal interneuron amacrine and Zn2+ then transferred to RGCs via transporter protein ZnT-3, triggering RGC loss injury. chelation ZnT-3 deletion promote long-term survival. However, downstream signaling pathways remains unknown. Here, we show that increased upregulate expression activity...
Antimicrobial peptides (AMPs) are traditionally known to be essential components in host defense via their broad activities against bacteria, fungi, viruses, and protozoa. Their immunomodulatory properties have also recently received considerable attention mammalian somatic tissues of various species. However, little is regarding the role AMPs development maturation ovarian follicles. Protegrin-1 (PG-1) an antimicrobial peptide which potent activity both gram positive negative bacteria. Here...
Retinal ischaemia/reperfusion (I/R) injury is a common cause of retinal ganglion cell (RGC) apoptosis and axonal degeneration, resulting in irreversible visual impairment. However, there are no available neuroprotective neurorestorative therapies for I/R injury, more effective therapeutic approaches needed. The role the myelin sheath optic nerve after remains unknown. Here, we report that demyelination an early pathological feature identify sphingosine-1-phosphate receptor 2 (S1PR2) as...
tet(X4) is the critical resistance gene for tigecycline degradation that has been continually reported in recent years. In particular, pathogenic bacteria carrying are a severe threat to human health. However, information describing Escherichia coli coharboring with virulence genes limited. Here, we isolated an E. strain and heat-stable toxin astA from dead piglet. The named 812A1-131 belongs ST10. genome was sequenced using Nanopore Illumina platforms. located on chromosome IncHI1-type...
Our study explores zinc’s pivotal role in mitigating extracellular matrix dysregulation the trabecular meshwork and glucocorticoid-induced ocular hypertension. We found that human cells treated with dexamethasone, intracellular Zn 2+ significantly decreased, accompanied by impaired uptake. Zinc supplementation rescues visual function modulating proteins lowering intraocular pressure, offering a direction for further exploration glaucoma management.
Antimicrobial peptides (AMPs) are regarded as host defense that possess bactericidal activity well immunomodulatory function. However, the role of AMP in mammalian ovary is unknown. In present study, porcine granulosa cells were utilized a cell model to study beta defensin 2 (pBD2; pDEFB4B) and 3 (pBD3; pDEFB103A) during ovarian follicular development. Granulosa cultured absence presence 1, 10, 50 μg/ml pDEFB4B or pDEFB103A. After 24 h treatment, pDEFB103A but not stimulated proliferation...
Abstract Introduction Non-ophthalmologists often lack sufficient operational training to use a direct ophthalmoscope proficiently, resulting in global deficit of basic ophthalmological skills among general practitioners. This deficiency hampers the timely diagnosis, referral, and intervention patients. Consequently, optimization teaching tools methods enhance efficiency is imperative. study explores effectiveness Eyesi Direct Ophthalmoscope Simulator (Eyesi) as an innovative tool for fundus...
Abstract Avian pathogenic Escherichia coli is an important etiological agent of avian colibacillosis, which manifests as respiratory, hematogenous, meningitic, and enteric infections in poultry. It also a potential zoonotic threat to human health. The diverse genomes APEC strains largely hinder disease prevention control measures. In the current study, pyrosequencing was used analyze characterize strain ACN001 (= CCTCC 2015182 T = DSMZ 29979 ), isolated from liver diseased chicken China...
Recent studies have reported that T-reg cells are intimately linked with hair follicles in a stage-dependent manner and play an important role follicle cycling regeneration murine skin. Further study revealed cell's regulation of growth is through its preferential expression the Notch ligand Jagged‐1 (Jag1), which facilitates regeneration. However, Jag1 androgen-suppressed yet to be investigated. In addition, although epidermal factor (EGF) mitogen for including skin cells, whether it works...
Staphylococcus warneri is a coagulase-negative staphylococcus that normal inhabitant of the skin. It also considered to be an opportunistic etiological agent causing significant infections in human and animals. Currently, relatively little attention has been paid genome biology S. pathogenicity antibiotic resistance, which are emerging issues for this with considerably clinical significance. In study, we determined complete sequence strain GD01 recovered from sampled muscle abscess tissue...