A5100323182- CRISPR and Genetic Engineering
- Crop Yield and Soil Fertility
- Chromosomal and Genetic Variations
- Ruminant Nutrition and Digestive Physiology
- Gut microbiota and health
- Genomic variations and chromosomal abnormalities
- Coccidia and coccidiosis research
- Animal Genetics and Reproduction
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Meat and Animal Product Quality
- Fungal and yeast genetics research
- Urological Disorders and Treatments
- Cancer-related molecular mechanisms research
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Animal Nutrition and Physiology
- Molecular Biology Techniques and Applications
- Helminth infection and control
- Spondyloarthritis Studies and Treatments
- Plant Micronutrient Interactions and Effects
- Insect symbiosis and bacterial influences
- Veterinary medicine and infectious diseases
- Hair Growth and Disorders
South China Normal University
2025
Capital Normal University
2023-2024
Northwest A&F University
2016-2024
Ministry of Agriculture and Rural Affairs
2015-2024
ETH Zurich
2022-2024
Anhui Agricultural University
2024
Tianjin University of Traditional Chinese Medicine
2024
First Hospital of Shijiazhuang
2024
Hebei Agricultural University
2024
Yunnan Agricultural University
2024
Nucleotide base editors in plants have been limited to conversion of cytosine thymine. Here, we describe a new plant adenine editor based on an evolved tRNA adenosine deaminase fused the nickase CRISPR/Cas9, enabling A•T G•C at frequencies up 7.5% protoplasts and 59.1% regenerated rice wheat plants. An endogenous gene is also successfully modified through introducing gain-of-function point mutation directly produce herbicide-tolerant plant. With this editing system, it now possible precisely...
In the process of green and smart mine construction under context carbon neutrality, China's coal safety situation has been continuously improved in recent years. order to recognize development production China prepare for future monitoring prevention incidents, this study mainly elaborated on basic resources national mining accidents over past five years (2017-2021), from four dimensions (accident level, type, region, time), then proposed preventive measures based accident statistical laws....
The increasing amount of sequencing data available for a wide variety species can be theoretically used detecting copy number variations (CNVs) at the population level. However, growing sample sizes and divergent complexity nonhuman genomes challenge efficiency robustness current human-oriented CNV detection methods. Here, we present CNVcaller, read-depth method discovering CNVs in data. computational speed CNVcaller was 1–2 orders magnitude faster than CNVnator Genome STRiP complex with...
Periodontitis is an inflammatory disease affecting the tissues supporting teeth (periodontium). Integrative analysis of metagenomic samples from multiple periodontitis studies a powerful way to examine microbiota diversity and interactions within host oral cavity. A total 43 subjects were recruited participate in two previous profiling microbial community human subgingival plaque using shotgun sequencing. We integrated sequence data those studies, including six healthy controls, 14 sites...
Bacterial communities in gastrointestinal tracts (GIT) play an important role animal health and performance. Despite its importance, little information is available on the establishment of microbial populations goat GIT or changes occurring during early development. Therefore, this study investigated bacterial community dynamics rumen, duodenum, jejunum, ileum, cecum, colon 15 goats at five developmental stages (0, 14, 28, 42, 56 days old) by using 16S rDNA sequencing quantitative real-time...
Massive Open Online Courses (MOOCs) is an innovative method in modern education, especially important for autonomous study and the sharing of global excellent education resources. However, it not easy to implement teaching process according specific characters students by MOOCs because number participants huge teacher cannot identify through a face interaction. As new subject combined with different areas, such as economics, sociology, environment, even engineering, sustainability-related...
Summary Heading date (or flowering time) is a key agronomic trait that affects seasonal and regional adaption of rice cultivars. An unoptimized heading can either not achieve high yield or has risk encountering abiotic stresses. There strong demand on the mild to moderate adjusting in breeding practice. Genome editing promising method which allows more precise faster changing rice. However, direct knock out major genes involved regulating will always new germplasm with expected date. It...
Changes in Warner–Bratzler shear force, cooking loss, protein solubility and microstructure of duck breast muscle cooked to 40, 50, 60, 70, 80, 90 95 °C were determined. force was found increase two separate phases from internal temperature 40–50 again 60 (P < 0.05), with a decrease 50 °C. With increasing loss gradually increased while significantly decreased 0.05). Two significant decreases fiber diameter observed samples temperatures 0.01) 70–80 0.01). The sarcomere length for the most...
Abnormally high activation of transforming growth factor-β (TGF-β) signaling has been demonstrated to be involved in the initiation and progression keloids. However, functional role long non-coding RNA (lncRNA)-activated by TGF-β (lncRNA-ATB) keloids not documented. Here we investigated lncRNA-ATB autocrine secretion keloid fibroblasts (KFs) explored underlying molecular mechanism. Using immunohistochemistry quantitative RT-PCR analysis, showed that ZNF217, a transcriptional activator TGF-β,...
Abstract We describe here a CRISPR simultaneous and wide-editing induced by single system (SWISS), in which RNA aptamers engineered crRNA scaffold recruit their cognate binding proteins fused with cytidine deaminase adenosine to Cas9 nickase target sites generate multiplexed base editing. By using paired sgRNAs, SWISS can produce insertions/deletions addition Rice mutants are generated the efficiencies of cytosine conversion 25.5%, adenine 16.4%, indels 52.7%, triple mutations 7.3%. The...
ABSTRACT In vivo genetic mutation has become a powerful tool for dissecting gene function; however, multi-gene interaction and the compensatory mechanisms involved can make findings from single mutations, at best difficult to interpret, and, worst, misleading. Hence, it is necessary establish an efficient way disrupt multiple genes simultaneously. CRISPR/Cas9-mediated base editing disrupts function by converting protein-coding sequence into stop codon; this referred as CRISPR-stop. Its...
Improvement of water-use efficiency (WUE) can effectively reduce production losses caused by drought stress. A better understanding the genetic determination WUE in crops under stress has great potential value for developing cultivars adapted to arid regions. To identify loci associated with and reveal genes responsible trait apple, we aim map quantitative (QTLs) carbon isotope composition, proxy WUE, applying two contrasting irrigating regimes over two-year experiment search candidate...
Abstract Understanding the genetic mechanism of how animals adapt to extreme conditions is fundamental determine relationship between molecular evolution and changing environments. Goat one first domesticated species has evolved rapidly diverse environments, including harsh high-altitude with low temperature poor oxygen supply but strong ultraviolet radiation. Here, we analyzed 331 genomes domestic goats wild caprid living at varying altitudes (high &gt; 3000 m above sea level &lt;...
CRISPR-based directed evolution is an effective breeding biotechnology to improve agronomic traits in plants. However, its gene diversification still limited using individual single guide RNA. We described here a multiplexed orthogonal base editor (MoBE), and randomly sgRNAs assembly strategy maximize diversification. MoBE could induce efficiently ABE (<36.6%), CBE (<36.0%), A&CBE (<37.6%) on different targets, while the sgRNA assembling randomized editing events various targets. With...
Here we report the use of exonuclease to expose complementary DNA between an insert and vector such that annealing becomes independent restriction site compatibility. We demonstrate unusual and, in some cases, previously impossible cloning strategies can be readily efficiently achieved as long flanking sequences linear vectors are highly related. Furthermore, show bacterial repair system resolves residual mismatches, overhangs or gaps a predictable fashion generate excisable inserts. This...
Dehydrins (DHNs) play a crucial role in enhancing abiotic stress tolerance plants. Although DHNs have been identified and characterized many plants, there is little known about Capsicum annuum L., one of the economically important vegetable crops. In this study, seven CaDHNs pepper genome were identified, which could be divided into two classes: YnSKn- SKn-type, based on their highly conserved domains. Quantitative real-time PCR (qRT-PCR) results showed that DHN genes expressed all tissues...
ABSTRACT Coccidiosis, caused by different species of Eimeria parasites, is an economically important disease poultry and livestock worldwide. Here we report previously unknown alterations in the gut microbes metabolism BALB/c mice infected with falciformis . Specifically, observed a significant shift abundance cecal bacteria disrupted parasitized animals. The relative abundances Lachnospiraceae bacterium NK4A136, Ruminiclostridium , Alistipes Lactobacillus declined response to E. infection,...
Ruminant animals possess a characteristic four-compartment stomach (rumen, reticulum, omasum, and abomasum) that is specialized for pre-intestinal digestion of plant materials. Of these four compartments, the rumen largest. The rumen's diverse microbial community has been well studied. However, current understanding profiles in omasum abomasum are lacking. In present study, fluid samples from goats at 3, 7, 14, 21, 28, 42, 56 days after birth, as negative controls (NC) used DNA extraction,...