A5021713764- Cancer-related molecular mechanisms research
- Dermatologic Treatments and Research
- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA modifications and cancer
- Inflammatory Biomarkers in Disease Prognosis
- HER2/EGFR in Cancer Research
- Kruppel-like factors research
- Cancer Immunotherapy and Biomarkers
- Heat shock proteins research
- Immune cells in cancer
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Bone and Joint Diseases
- Peroxisome Proliferator-Activated Receptors
- Virus-based gene therapy research
- Trauma Management and Diagnosis
- Metal and Thin Film Mechanics
- Phagocytosis and Immune Regulation
- Ginseng Biological Effects and Applications
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- High Altitude and Hypoxia
- Liver physiology and pathology
Chinese People's Liberation Army
2016-2024
Air Force Medical University
2012-2022
Xijing Hospital
2021-2022
Urumqi General Hospital of PLA
2016-2021
Fujian Provincial Cancer Hospital
2019-2020
Fujian Medical University
2019-2020
Chinese General Hospital College of Nursing and Liberal Arts
2019
Zero to Three
2014
State Key Laboratory of Cancer Biology
2009
Resistance to trastuzumab and concomitantly distal metastasis are leading causes of mortality in HER2‐positive breast cancers, the molecular basis which remains largely unknown. Here, we generated trastuzumab‐resistant cancer cells with increased tumorigenicity invasiveness compared parental cells, observed robust epithelial–mesenchymal transition (EMT) consistently elevated TGF‐β signaling these cells. MiR‐200c, was most significantly downregulated miRNA restored sensitivity suppressed...
Resistance to humanized monoclonal erbB2/HER2 antibody, trastuzumab (Herceptin), has become a pivotal obstacle for targeted therapy of HER2-positive breast cancers. The activation alternative growth factor receptors, in particular, the insulin-like 1 receptor (IGF1R), represents common feature trastuzumab-refractory cells; however, underlying mechanism remains elusive. Trastuzumab-resistant cancer SKBr-3 cells were generated by long-term vitro culture presence trastuzumab. Among...
HER2-overexpressing breast cancers are characterized by frequent distant metastasis and often develop resistance after short-term effective treatment with the monoclonal antibody drug, trastuzumab. Here, we found that oncogenic miRNA, miR-221, inhibited apoptosis, induced trastuzumab promoted of HER2-positive cancers. The tumor suppressor PTEN was identified as a miR-221 target; overexpression abrogated aforementioned miR-221-induced malignant phenotypes cells. These findings indicate may...
Background As an important oncogenic miRNA, microRNA-21 (miR-21) is associated with various malignant diseases. However, the precise biological function of miR-21 and its molecular mechanism in hypertrophic scar fibroblast cells has not been fully elucidated. Methodology/Principal Findings Quantitative Real-Time PCR (qRT-PCR) analysis revealed significant upregulation compared that normal skin cells. The effects were then assessed MTT apoptosis assays through vitro transfection a mimic or...
Abnormally high activation of transforming growth factor-β (TGF-β) signaling has been demonstrated to be involved in the initiation and progression keloids. However, functional role long non-coding RNA (lncRNA)-activated by TGF-β (lncRNA-ATB) keloids not documented. Here we investigated lncRNA-ATB autocrine secretion keloid fibroblasts (KFs) explored underlying molecular mechanism. Using immunohistochemistry quantitative RT-PCR analysis, showed that ZNF217, a transcriptional activator TGF-β,...
Background: Hepatocellular carcinoma (HCC) is the most common type of liver cancers, with more than a million cases per year by 2025. Cuproptosis novel form programmed cell death, and caused mitochondrial lipoylation destabilization iron-sulfur proteins triggered copper, which was considered as key player in various biological processes. However, roles cuproptosis-related genes (CRGs) HCC remain largely unknown. Methods: In present study, we constructed validated four CRGs signature for...
Human epidermal growth factor receptor 2 (HER-2) was an important driver gene for breast cancer which had high degree of malignancy and poor prognosis. Ultrasonography imaging method the diagnosis cancer, but its diagnostic efficacy HER-2-positive not satisfactory. To assess predictive value two-dimensional ultrasonic feature extraction based on artificial intelligence (AI) combined with blood flow Adler classification contrast-enhanced ultrasound (CEUS) we compared area under receiver...
Altered expression levels of the proteins that regulate N6-methyladenosine (m 6 A) RNA methylation, including methyltransferase-like 14 (METTL14), are associated with cancer development. Based on our analysis m A methylation regulators using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets, we focused regulatory role METTL14 in ovarian cancer. We performed bioinformatics survival analyses these datasets also used METTL14-overexpressing SKOV-3 cells for vitro studies....
Urokinase type plasminogen activator (uPA) and inhibitor‐1 (PAI‐1) have been proposed to play key roles in extracellular matrix (ECM) deposition hypertrophic scars (HS). Here, we found that HS fibroblasts (HFs) miR‐181c miR‐10a were differentially‐expressed targeted uPA PAI‐1, respectively. The production of Type 1 collagen (Col1) was inhibited by knockdown or overexpression HFs, this resulted increased levels metalloproteinase (MMP1). These results suggest the miR‐181c–uPA miR‐10a–PAI‐1...
Abstract Recent microRNA expression profiling studies have documented an up-regulation of miR-146a in several angiogenesis models. However, the underlying molecular mechanism angiogenic activity endothelial cells has not been clearly elucidated. The present study was aimed to evaluate whether promotes HUVECs by increasing FGFBP1 via directly targeting CREB3L1. over expressed lentiviral-miR-146a. Expression analysis found resulted and cytokine associated genes including FGF2. Further a...
Recent studies have demonstrated maternally expressed gene 3 (MEG3) as a tumor suppressor across multiple malignancies. Meanwhile, the role of MEG3 in ovarian cancer needs further investigation. We aim to study effects on angiogenesis and underlying mechanisms.The transcript levels samples from GEPIA database were analyzed compared those normal samples. The effect tube formation ability was quantified carcinoma-derived microvascular endothelial cells (ODMECs). Through sequence analysis, we...
Rg1 is a predominant protopanaxatriol-type of ginsenoside found in Panax ginseng, and it has been shown to have anti-cancer effects multiple types cancer cells. However, also induces the expression proangiogenic factors, such as vascular endothelial growth factor (VEGF-A), Unfortunately, angiogenesis positively correlates with development. In this study, we identified RUNX2 regulator Rg1-induced for first time. We that was directly targeted regulated by miR-23a. Additionally, miR-23a inhibit...
Glucocorticoid counteracts cellular mechanoresponses by a novel lncRNA-dependent mRNA decay machinery.
Background: The oncogene IGF2 mRNA binding protein 3 (IGF2BP3) could function as an m 6 A reader in stabilizing many tumor-associated genes’ mRNAs. However, the relevant oncogenic mechanism by which IGF2BP3 promotes ovarian cancer growth is largely unknown. Methods: expression was identified retrieving datasets from Cancer Genome Atlas (TCGA). GEO evaluated signaling pathways knockdown cells. positive correlation gene TCGA calculated. MTS proliferation assay and rescued PLAG1 like zinc...
Resistance to human epidermal growth factor receptor 2 (HER2)-targeted therapy (trastuzumab), cancer stem cell (CSC)-like properties and multiple chemoresistance often concur intersect in breast cancer, but molecular links that may serve as effective therapeutic targets remain largely unknown. Here, we identified the long noncoding RNA, LINC00589 a key regulatory node for concurrent intervention of these processes cells vitro vivo. We demonstrated expression is clinically valuable an...
Inhibitors of histone deacetylases (HDACi) have shown promising effects in preclinical applications for the treatment many diseases. Confusedly though, HDACi trichostatin A (TSA) on angiogenesis are variable among different This study investigated direct TSA endothelial cells, which plays essential roles and underlying molecular events. reduced viability human umbilical vein cells (HUVECs), proliferation-related genes including BIRC5 , CKS1B NDC80 were found to be involved. Furthermore,...
The abnormal upregulation of transforming growth factor-β (TGF-β) signaling has been demonstrated to initiate keloid formation and progression. Keloid is a type benign skin tumor that may occur following sustaining injury. microRNA-96 (miR-96) serves an important role in the progression various malignant diseases. Using reverse transcription quantitative polymerase chain reaction (RT-qPCR), present study miR-96 was overexpressed keloid-derived fibroblasts (KFs). Luciferase reporter assay...
// Hua-Yu Zhu 1, * , Wen-Dong Bai 2, Chao Li 3, Jun 1 and Da-Hai Hu Department of Burns Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, P.R. China 2 Clinical Laboratory Center, Xinjiang Command General Hospital Chinese People’s Liberation Army, Urumqi, Xinjiang, 3 Center Plastic Lanzhou Lanzhou, Gansu, These authors have contributed equally to this work Correspondence to: Hu, email: zzhuhy@aliyun.com Li, lijunfmmu@163.com Keywords:...
The development of diseases seems to be related several mediators.Drugs targeting one component a biological system may not achieve desired clinical effects.Multiple antigen-targeting strategies,including combination antibodies,polyclonal antibodies or antibody mixtures,and bispecific antibodies,are promising ones increase therapeutic efficacy for treatment certain complex diseases.However,dual-andpan-specific monoclonal developed in recent years provide novel approach multiple...