A5015716640- Genetic and phenotypic traits in livestock
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Genomic variations and chromosomal abnormalities
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Genomics and Rare Diseases
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Tryptophan and brain disorders
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Metabolomics and Mass Spectrometry Studies
- Skin and Cellular Biology Research
- Identification and Quantification in Food
- Ruminant Nutrition and Digestive Physiology
- Hair Growth and Disorders
- Speech and Audio Processing
- Stress Responses and Cortisol
- Meta-analysis and systematic reviews
- Speech Recognition and Synthesis
- Pulsars and Gravitational Waves Research
- Hepatitis C virus research
Northwest A&F University
2016-2025
Beijing University of Chinese Medicine
2021-2024
Agriculture and Forestry University
2019-2024
First Affiliated Hospital of Henan University
2024
Tianjin University of Traditional Chinese Medicine
2021-2024
Dalian University of Technology
2024
Sichuan University
2024
The University of Tokyo
2024
Sanya University
2022-2023
Shandong First Medical University
2022
Structural variations (SVs) are a major contributor to genetic diversity and phenotypic variations, but their prevalence functions in domestic animals largely unexplored. Here we generated high-quality genome assemblies for 15 individuals from genetically diverse sheep breeds using Pacific Biosciences (PacBio) high-fidelity sequencing, discovering 130.3 Mb nonreference sequences, which 588 genes were annotated. A total of 149,158 biallelic insertions/deletions, 6531 divergent alleles, 14,707...
According to historical records of transatlantic slavery, traders forcibly deported an estimated 12.5 million people from ports along the Atlantic coastline Africa between 16th and 19th centuries, with global impacts reaching present day, more than a century half after slavery's abolition. Such have fueled broad understanding forced migration Americas yet remain underexplored in concert genetic data. Here, we analyzed genotype array data 50,281 research participants, which—combined shipping...
Goat domestication was critical for agriculture and civilization, but its underlying genetic changes selection regimes remain unclear. Here, we analyze the genomes of worldwide domestic goats, wild caprid species, historical remains, providing evidence an ancient introgression event from a West Caucasian tur-like species to ancestor goats. One introgressed locus with strong signature harbors MUC6 gene, which encodes gastrointestinally secreted mucin. Experiments revealed that nearly fixed...
The increasing amount of sequencing data available for a wide variety species can be theoretically used detecting copy number variations (CNVs) at the population level. However, growing sample sizes and divergent complexity nonhuman genomes challenge efficiency robustness current human-oriented CNV detection methods. Here, we present CNVcaller, read-depth method discovering CNVs in data. computational speed CNVcaller was 1–2 orders magnitude faster than CNVnator Genome STRiP complex with...
The gene numbers and evolutionary rates of birds were assumed to be much lower than those mammals, which is in sharp contrast the huge species number morphological diversity birds. It is, therefore, necessary construct a complete avian genome analyze its evolution. We constructed chicken pan-genome from 20 de novo assembled genomes with high sequencing depth, identified 1,335 protein-coding genes 3,011 long noncoding RNAs not found GRCg6a. majority these novel detected across most...
The worldwide sheep population comprises more than 1000 breeds. Together, these exhibit a considerable morphological diversity, which has not been extensively investigated at the molecular level. Here, we analyze whole-genome sequencing individuals of 1,098 domestic from 154 breeds, and 69 wild seven Ovis species. On average, detected 6.8%, 1.0% 0.2% introgressed sequence in originating Iranian mouflon, urial argali, respectively, with rare introgressions other Interestingly, several...
The steroid receptor RNA activator (SRA) has previously been characterized as belonging to the growing family of functional non-coding RNAs. However, we recently reported Western blot detection a putative endogenous SRA protein (SRAP) in breast cancer cells. Herein, successfully suppressed expression this through specific interference assay, unequivocally confirming its existence. Moreover, using database searches and analysis, also showed that SRAP is highly conserved among chordata....
Genetic engineering in livestock has been greatly enhanced through the use of artificial programmed nucleases such as recently emerged clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system. We reported our successful application CRISPR/Cas9 system to engineer goat genome micro-injection Cas9 mRNA and sgRNAs targeting MSTN FGF5 embryos. The phenotypes induced by edited loss-of-function mutations remain be evaluated extensively. demonstrate...
Xiaoyao pills (XYP) is a commercial Chinese patent medicine used in the treatment of depression. However, mechanisms underlying its therapeutic effects, as well patients who can benefit from XYP, have not been evaluated so far.
Goats are one of the most widespread farmed animals across world; however, their migration route to East Asia and local evolutionary history remain poorly understood. Here, we sequenced 27 ancient Chinese goat genomes dating from Late Neolithic period Iron Age. We found close genetic affinities between modern goats, demonstrating continuity. that goats originated eastern regions around Fertile Crescent, estimated ancestors diverged this population in Chalcolithic period. Modern were divided...
Abstract Understanding the genetic mechanism of how animals adapt to extreme conditions is fundamental determine relationship between molecular evolution and changing environments. Goat one first domesticated species has evolved rapidly diverse environments, including harsh high-altitude with low temperature poor oxygen supply but strong ultraviolet radiation. Here, we analyzed 331 genomes domestic goats wild caprid living at varying altitudes (high > 3000 m above sea level <...
Background: Paeoniflorin (PF) represents the major bioactive constituent of traditional Chinese medicine plant Paeonia suffruticosa (Ranunculaceae), which has a long history as folk in Asian. Paeoniflorin, bitter pinene monoterpene glycoside, antidepressant effects, but its potential therapeutic mechanism not been thoroughly explored. Methods: Experimental depression rats was established by chronic unpredictable mild stress (CUMS) combined with orphan method, and efficacy paeoniflorin on...
Chinese indicine cattle harbor a much higher genetic diversity compared with other domestic cattle, but their genome architecture remains uninvestigated. Using PacBio HiFi sequencing data from 10 across southern China, we assembled 20 high-quality partially phased genomes and integrated them into multiassembly graph containing 148.5 Mb (5.6%) of novel sequence. We identified 156,009 high-confidence nonredundant structural variants (SVs) 206 SV hotspots spanning ∼195 gene-rich detected 34,249...
Using ancestral recombination graphs, we investigated recent selection signatures in European beef cattle breeds, pinpointing sweep-driving variants the LCORL and STC2 loci with notable effects on body size growth rate. The ACT-to-A frameshift mutation occurs mainly central-European cattle, stimulates growth. Remarkably, similar truncating mutations were found seven of other most important domesticated species, but not populations wild relatives. In locus, identified a missense C-to-G...
Goats are essential to the dairy industry in Shaanxi, China, with udder traits playing a critical role determining milk production and economic value for breeding programs. However, direct measurement of these goats is challenging resource-intensive. This study leveraged genotyping imputation explore genetic parameters architecture assess efficiency genomic prediction methods. Using data from 635 Saanen goats, genotyped over 14,717,075 SNP markers phenotyped three traits, heritability was...
Abstracts Natural variants can significantly improve growth traits in livestock and serve as safe targets for gene editing, thus being applied animal molecular designed breeding. However, such large-effect mutations are severely lacking. Using ancestral recombination graphs, we investigated recent selection signatures beef cattle breeds, pinpointing sweep-driving the LCORL STC2 loci with notable effects on body size rate. The ACT-to-A frameshift mutation occurs mainly central-European...
It is broadly expected that next generation sequencing will ultimately generate a complete genome as the latest goat reference (ARS1), which considered to be one of most continuous assemblies in livestock. However, rich diversity worldwide breeds indicates from individual would insufficient represent whole genomic contents goats. By comparing nine de novo seven sibling species domestic with ARS1 and using resequencing transcriptome data goats for verification, we identified total 38.3 Mb...
Background: Depression is a stress-related disorder that seriously threatens people's physical and mental health. Xiaoyaosan classical traditional Chinese medicine formula, which has been used to treat depression since ancient times. More more notice given the relationship between occurrence of necroptosis pathogenesis disorders. Objective: The purpose present study explore potential mechanism for treatment using network pharmacology experimental research, identify targets underlying...
Abstract Background Goat, one of the first domesticated livestock, is a worldwide important species both culturally and economically. The current goat reference genome, known as ARS1, reported nonhuman genome assembly using 69× PacBio sequencing. However, ARS1 suffers from incomplete X chromosome highly fragmented Y scaffolds. Results Here, we present very high-quality de novo assembly, Saanen_v1, male Saanen dairy goat, with scaffold based on 117× long-read sequencing 118× Hi-C data....