A5069022753- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Livestock and Poultry Management
- Livestock Farming and Management
- Vector-Borne Animal Diseases
- Genetic diversity and population structure
- Effects of Environmental Stressors on Livestock
- Antimicrobial Peptides and Activities
- Genomic variations and chromosomal abnormalities
- Immune Response and Inflammation
- Chromosomal and Genetic Variations
- Synthesis and Characterization of Heterocyclic Compounds
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Animal Genetics and Reproduction
- Silkworms and Sericulture Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Protein Hydrolysis and Bioactive Peptides
- Heat shock proteins research
- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Synthesis and biological activity
- Adipose Tissue and Metabolism
- Quinazolinone synthesis and applications
National Institute for Biotechnology and Genetic Engineering
2018-2023
Northwest A&F University
2020-2022
Pakistan Institute of Engineering and Applied Sciences
2018-2021
NED University of Engineering and Technology
2021
International Center for Chemical and Biological Sciences
2021
University of Karachi
2021
Academe of Grassland And Animal Science
2020
China Agricultural University
2018-2019
Indicine cattle, also referred to as zebu (Bos taurus indicus), play a central role in pastoral communities across wide range of agro-ecosystems, from extremely hot semiarid regions humid tropical regions. However, their adaptive genetic changes following dispersal into East Asia the Indian subcontinent have remained poorly documented. Here, we characterize global diversity using high-quality whole-genome sequencing data 354 indicine cattle 57 breeds/populations, including major...
The single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) are two major genomic variants, which play crucial roles in evolutionary phenotypic diversity.In this study, we performed a comprehensive analysis to explore the genetic (SNPs CNVs) of high sperm motility (HSM) poor (PSM) Simmental bulls using high-coverage (25×) short-read next generation sequencing single-molecule long reads data. A total ~ 15 million SNPs 2,944 CNV regions (CNVRs) were detected bulls, set...
The primary goal of cattle genomics is the identification genome-wide polymorphism associated with economically important traits. bovine genome sequencing project was completed in 2009. Since then, using massively parallel technologies, a large number Bos taurus breeds have been resequenced and scanned for polymorphisms. As result, substantial single nucleotide polymorphisms (SNPs) discovered across European genomes, whereas extremely less SNPs are cataloged indicus breeds. In this study, we...
Yanbian cattle is inhabitant of North China, exhibiting many phenotypic features, such as long, dense body hair, and abundant intramuscular fat, designed to combat the extreme cold climate adaption. In current study, we studied tolerance nine by whole genome resequencing compared with African tropical cattle, N'Dama, a control group. was aligned Bos taurus reference (ARS-UCD1.2) yielding an average 10.8 fold coverage. The positive selective sweep analysis for adaption in were analyzed using...
Abstract Yunling cattle, a three‐breed cross consisting of 1/2 Brahman 1/4 Murray Grey cattle and Yunnan Yellow has advantage rapid growth, good meat quality, enhanced tolerance towards hot humid climate, tick resistance crude feed forbearance. Here, we investigated the genetic diversity, global local ancestry proportions by sequencing genomes 131 Yungling 31 (for control). Furthermore, used 35 published breeds (including Angus [ancestry Grey], Wannan cattle: Wenshan Dianzhong], Dianzhong...
Abstract Background Fuzhong buffalo, a native breed of Guangxi Zhuang Autonomous Region, is traditionally used as draft animal to provide farm power in the rice cultivation. In addition, buffalo also prepared for bullfighting festival organized by locals. The detection selective signatures its genome can help elucidating selection mechanisms stamina and muscle development animal. Results this study, we analyzed 27 whole genomes (including 15 12 published from Upper Yangtze region). ZHp,...
This study pertains to the new approach for development of hybrid peptide LL-37Tα1 and its biomedical applications. A linear cationic peptide, was derived from two parental peptides (LL-37 Tα1) recognized as potent anti-endotoxin without any hemolytic or cytotoxic activity. We successfully cloned gene in PpICZαA vector expressed P. pastoris. The recombinant purified by Ni-affinity column reverse-phase high performance liquid chromatography (RP-HPLC) with an estimated molecular mass 3.9 kDa...
Summary Ketosis ( KET ) is one common metabolic disorder that occurs mainly in early lactation and affects the dairy industry with significant economic losses. Cows ketosis have lower milk yield reproductive performance greater risk of other periparturient diseases. As a disease, pathogenesis multifactorial. To better understand genetic background , genome‐wide association study was performed using Illumina Bovine SNP 50 BeadChip. Single‐step genomic BLUP methodology used to incorporate data...
Based on the previous GWAS research related to bovine heat tolerance trait, this study aimed explore effect of myosin-1a (MYO1A) gene and find molecular markers Chinese cattle. In our study, four novel candidate SNPs highly conserved in B. indicus breeds but barely existed taurus were identified MYO1A according Bovine Genome Variation Database Selective Signatures (BGVD). PCR DNA sequencing used genotype 1072 individuals including 34 indigenous cattle as well Angus Indian zebu. Two...
Chaka sheep, named after Salt Lake, are adapted to a harsh, highly saline environment. They known for their high-grade meat quality and valuable genetic resource in China. Furthermore, the sheep breed has been designated geographical symbol of agricultural products by Chinese Ministry Agriculture.The genomes 10 were sequenced using next-generation sequencing, compared that additional breeds (Mongolian: Bayinbuluke Tan; Tibetan: Oula sheep) explore its population structure, diversity positive...
Stature is an important quantitative trait for cattle performance, which influences herd productivity. Previous studies have reported that SNP (AC_000171.1:g.25015640G>T, rs109815800) in Pleomorphic adenoma gene 1 (PLAG1) on chromosome 14 (CHR14) associated with bovine stature. To validate whether rs109815800 the body height of Chinese cattle, we carried out association analysis using 558 adult samples from seven populations. Then, 1038 38 breeds were used to show geographical distribution...
Guangxi Province is located in the southernmost region of China, adjacent to hotspot that considered be putative migratory corridor or domestication area for Chinese indicine cattle. Here, we investigated evolutionary status native breeds, Longlin (n = 21), Nandan 18), and Weizhou cattle 17) using mitogenome sequencing. Our results show Bos indicus sub-haplogroup I1a predominates breeds. Population structure by multidimensional-scaling analysis significantly differentiates from other two...
European taurine and East Asian are two main clades in Bos taurus, but their genomic differences not clearly elucidated. Here, we sequenced 16 Mongolian cattle genomes compared them to the 92 of 10 representative breeds worldwide. We found highest LD level Mishima fastest decay taurine. Phylogenetic analysis revealed that Mongolian, Hanwoo were clustered into From selective sweep, gene annotation, functional enrichment differential expression analysis, identified signals including genes...
The location on the Yunnan border with Myanmar and its unique cultural landscape has shaped Lincang humped cattle over time. In current study, we investigated genetic characteristics of 22 using whole-genome resequencing data. We found that derived from both Indian indicine Chinese depicted higher levels genomic diversity. Based genome-wide scans, candidate regions were identified potentially involved in local thermal humid environmental adaptions, including genes associated body size...
We report genome characterization of three native Chinese cattle breeds discovering ~34.3 M SNPs and ~3.8 InDels using whole resequencing. On average, 10.4 were shared amongst the breeds, whereas, 3.0 M, 4.9 5.8 specific to LQ, WN WS respectively. Gene ontology (GO)analysis revealed four immune response-related GO terms over represented in all samples, while two signaling pathways significantly over-represented cattle. Altogether, we found related genes (PGLYRP2, ROMO1, FYB2, CD46, TSC1)...
Wild yak (Bos mutus) is a vulnerable bovine species on the Qinghai-Tibetan Plateau (QTP). So far, most studies molecular genetic diversity of wild have focused autosomal and mtDNA variations based small number samples. In this study, we analyzed 84 D-loop 24 whole mitogenome sequences to further comprehensively explore its maternal lineage composition. Meanwhile, using six Y-specific polymorphic markers (i.e., SRY4, USP9Y, UTY19, AMELY3, OFD1Y10 INRA189), assessed paternal composition eight...
Dengchuan cattle are the only dairy yellow and endangered among Yunnan native breeds. However, its genetic background remains unclear. Here, we performed whole-genome sequencing of ten cattle. Integrating our data with publicly available data, were observed to be highly interbred than other in dataset. Furthermore, positive selective signals mainly manifested candidate genes pathways related milk production, disease resistance, growth development, heat tolerance. Notably, five (KRT39, PGR,...
The modern cattle was domesticated from aurochs, sharing its physiological traits into two subspecies Bos taurus and indicus. MicroRNAs (miRNAs) are a class of non-coding short RNAs ~22nt which have key role in the regulation many cellular processes animal. current study aimed to predict annotate potential mutations indicine miRNAs throughout genome using de novo homology-based silico approaches. Genome-wide mapping performed available assembly by approach 768 were recovered out 808 reported...
The production traits of cattle, especially milk trait, are great significance to human life. A quantitative trait loci (QTL) associated with fat content was detected in the centromeric region cattle chromosome 14. This QTL harbors a strong candidate gene called DGAT1 responsible for quality. non-conservative substitution lysine by alanine (K232A) found producing effect on composition and yield. (K allele) is increased content, while decreased linked (A amino acid. To estimate frequencies...
Copy number variations (CNVs) are an important source of genetic variations, which can affect a wide range economic traits by diverse mechanisms. Besides, genome scan has identified lots quantitative trait loci (QTL) about economically traits. Meanwhile, genome-wide association studies (GWAS) localize variants associated with phenotypic variations. Here, we developed method called GWAScore could collect GWAS date to identify positional candidate, and integrated CNVs into QTLs high confidence...
Abstract Lower flight reaction is closely related to higher production in cattle, but the genetic basis of lower not clearly understood. Here, we sampled a total 45 Brahman cattles and 166 Yunling with distance (FD), 73 288 crush score (CS) speed (FS), whereas there were 161 all three traits. The FD, CS FS cattle significantly than those cattle. traits had negative correlation conformational (e.g., body weight, withers height length). Based on SNPs derived from subset 162 whole genomes (25...
Cattle temperament is an interesting trait due to its correlation with production efficiency, labor safety, and animal welfare. To date, however, genetic basis not clearly understood. Here, we performed a genome-wide association study for series of traits in cattle, assessed via open field novel object tests, using autosomal single nucleotide polymorphisms (SNPs) derived from the whole-genome sequence. We identified 37 29 significant loci respectively. Gene set analysis revealed most pathway...