A5102733034- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Genetic and phenotypic traits in livestock
- MicroRNA in disease regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Effects of Environmental Stressors on Livestock
- S100 Proteins and Annexins
- Livestock Farming and Management
- Prion Diseases and Protein Misfolding
- RNA regulation and disease
- Lipid metabolism and disorders
- Congenital heart defects research
- Sperm and Testicular Function
- Lipid metabolism and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- RNA modifications and cancer
- Muscle Physiology and Disorders
- Heat shock proteins research
Ministry of Agriculture and Rural Affairs
2019-2025
Jilin Province Science and Technology Department
2025
Jilin Academy of Agricultural Sciences
1998-2025
Fanjingshan National Nature Reserve
2024
Animal Science Research Institute
2024
Yanbian University
2019
Institute of Animal Sciences
2018
Guilin Medical University
2015
Copy number variations (CNV) are important genetic variations. The endogenous factors cobalamin receptor (CUBN) and MIA SH3 domain ER-derived factor 3 (MIA3) associated with bone/muscle development intramuscular fat deposition. There have been no reports on the effects of CUBN MIA3 CNVs growth traits Chinese cattle. This study aimed to determine correlation between in qRT-PCR was used detect distribution CNV expression levels their mRNA, analysis conducted traits. differentially expressed...
Fatty acid composition (FA) is an important indicator of meat quality in beef cattle. We investigated potential functional candidate genes for FA cattle by integrating genomic and transcriptomic dataset through multiple strategies. In this study, we observed 65 SNPs overlapping with five (CCDC57, FASN, HDAC11, ALG14, ZMAT4) using two steps association based on the imputed sequencing variants. Using traits GWAS, further identified three significant located upstream FASN one SNP...
China exhibits a great diversity of ecosystems and abundant cattle resources, with nearly 30 million from 53 indigenous breeds reared in specific geographic regions. To explore the genetic population structure Chinese cattle, analysis at both individual levels was conducted admixture performed. We genotyped 572 samples 20 using GeneSeek Genomic Profiler Bovine LD (GGP-LD, K) downloaded published data 77 4 worldwide commercial Illumina BovineSNP50 Beadchip (SNP50, 50 K). In principal...
Yanbian cattle is inhabitant of North China, exhibiting many phenotypic features, such as long, dense body hair, and abundant intramuscular fat, designed to combat the extreme cold climate adaption. In current study, we studied tolerance nine by whole genome resequencing compared with African tropical cattle, N'Dama, a control group. was aligned Bos taurus reference (ARS-UCD1.2) yielding an average 10.8 fold coverage. The positive selective sweep analysis for adaption in were analyzed using...
MicroRNA (miRNA) is an important regulator of cell differentiation and function. Mechanical strain in the growth osteoblasts. Therefore, mechanresponsive miRNA may be response osteoblasts to mechanical strain. The purpose present study was select identify mechanoresponsive miRNAs Mouse osteoblastic MC3T3‑E1 cells were cultured culture dishes stimulated with a tensile 2,50 µε at 0.5 Hz, activity alkaline phosphatase (ALP), mRNA levels ALP, osteocalcin (OCN), collagen type I (Col I), protein...
In this study, we performed high throughput RNA sequencing at the primary bovine preadipocyte (Day-0), mid-differentiation (Day-4), and differentiated adipocyte (Day-9) stages in order to characterize transcriptional events regulating differentiation function. The preadipocytes were isolated from subcutaneous fetal adipose tissues into mature adipocytes. adipogenic characteristics of adipocytes detected during various adipogenesis (Day-0, Day-4, Day-9). We used (RNA-seq) investigate a...
The small heat shock proteins (HSPB) are expressed in response to stress, and the protein family B (small) member 7 (HSPB7) gene has been reported play an important role tolerance pathways. Only a missense mutation (NC_037329.1: g.136054902 C > G: p.Ala69Gly) was identified HSPB7 indicine cattle, which might be candidate associated with tolerance. Here, we explore allele frequency of this 774 individuals belonging 32 Chinese indigenous cattle breeds using polymerase chain reaction (PCR) DNA...
This study aims to screen potential regulators and regulate fecundity networks between microRNAs (miRNAs) target genes. The bovine testes of immature mature Chinese Red Steppes were performed by genome-wide analysis mRNAs miRNAs. Compared with testicular tissues newborns, 6051 upregulated genes 7104 downregulated in adult cattle identified as differentially expressed (DEGs). DEGs significantly enriched 808 GO terms (p < 0.05) including male gonad development, genitalia spermatogenesis, sperm...
The insulin-like growth factor binding-protein 7 (IGFBP7) has binding affinities to IGFs and is able either positively or negatively regulate the signaling pathway. It also plays a crucial role in cell growth, differentiation development an IGF-independent manner. Herein, we investigated specific regulation of gene encoding for IGFBP7during process adipocyte cells Yan Yellow Cattle by interfering with overexpressing IGFBP7 gene. As result, found that mRNA expression levels were significantly...
Alternative splicing is a ubiquitous regulatory mechanism in gene expression that allows single generating multiple messenger RNAs (mRNAs). Significant differences fat deposition ability and meat quality traits have been reported between Japanese black cattle (Wagyu) Chinese Red Steppes, which presented unique model for analyzing the effects of transcriptional level on marbling livestock. In previous studies, differentially expressed genes (DGEs) longissimus dorsi muscle ( LDM ) samples...
Objective Puerarin has the potential of regulating differentiation preadipocytes, but its mechanism action not yet been elucidated. Adipocytes found in adipose tissue, main endocrine organ, are sites lipid deposition, and widely used as a cell model study vitro fat deposition. This aimed to investigate effects puerarin on adipogenesis vitro. Methods was added culture medium during process adipogenesis. The proliferation bovine preadipocytes measured through viability staining with oil red O....
Chinese indigenous cattle breeds have abundant genetic resources, which are valuable for the molecular breeding of around world. Thus, identifying important candidate genes and their markers is very breeding. A previous study found that a nonsense mutation (rs378652941, c.483C > A, p.Cys161X) in bovine transmembrane protein 95 gene (TMEM95) seriously reduced reproductive performance bulls, but few studies detected this breeds. Since may serve as potential marker selecting higher fertility...
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cytosolic enzyme that mainly catalyzes the oxidation of acetaldehyde into acetic acid and participates in regulation differentiation gene expression fat cell growth development. However, physiological role ALDH1A1 formation precursors Yan Yellow Cattle still not clear. Herein, we investigated specific encoding for during process adipocyte cells by interfering or overexpressing gene. As result, found mRNA levels were significantly increased progenitor...
The Xinjiang brown cattle, Red steppe and Yunling cattle are indigenous cultivated breeds in Chinese frontier provinces, they produce high-grade beef milk products, however, their genetic diversity many important genes related to excellent meat production is still unknown. Our previous studies have found that several candidate (e.g., SREBP1c PAX7) were associated with bovine economically phenotypic traits, but none has been reported the above-mentioned three breeds. Since InDel...
In this study, we applied thermal dissipation probe technology to examine sap flow in various directions (east, south, west, and north) at different depths (0-2, 2-4, 4-6 cm) within the stem of natural
Background: Cultivated meat, an alternative to conventional has substantial potential for alleviating environmental and ethical concerns. This method of manufacturing meat involves the isolation skeletal muscle satellite cells (SMSCs) from donor animals, after which they proliferate in vitro differentiate into primitive fibers. The aim this research was evaluate how insulin-like growth factor 1 (IGF1) gene regulates myogenic differentiation bovine (bSMSCs). Methods: bSMSCs isolated newborn...
Three different strains of pseudorabies (Aujeszky's disease) virus were inoculated into ligated closed loops in the jejunum and ileum five specific-pathogen-free pigs. Infected areas compared with respect to distribution histologic lesions antigen. Two wild-type produced enteric consisting necrosis subepithelial macrophages basilar crypt epithelium, lymphoid follicles Peyer's patches, degeneration epithelial cells villi, neuronal myenteric plexuses, formation intranuclear inclusion bodies on...
Objective To study the effect of transcranial magnetic stimulation on migration phosphoprotein-enriched astrocytes-15kDa (PEA-15). Methods Third or fourth generation rat astrocytes cultured in vitro were divided into a control group, transfected group and + group. The was undergone transfect negative siRNA. In liposome astrocyte instantly with chemically synthesized PEA-15 siRNA, so as to interfere expression protein. Magnetic applied both tranfected groups 24 h after plating at 1 Hz...