A5019265757- Cancer-related molecular mechanisms research
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- RNA Research and Splicing
- RNA modifications and cancer
- MicroRNA in disease regulation
- Muscle Physiology and Disorders
- Circular RNAs in diseases
- Cancer-related gene regulation
- Congenital heart defects research
- Kruppel-like factors research
- Epigenetics and DNA Methylation
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- TGF-β signaling in diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Genetic Syndromes and Imprinting
- Tissue Engineering and Regenerative Medicine
- Genetic Mapping and Diversity in Plants and Animals
- Spaceflight effects on biology
- Developmental Biology and Gene Regulation
- Osteoarthritis Treatment and Mechanisms
Shanxi Agricultural University
2025
Anhui Agricultural University
2022-2024
Poultry Research Institute
2024
Northwest A&F University
2015-2023
Jilin Academy of Agricultural Sciences
2019
Ministry of Agriculture and Rural Affairs
2019
Google (United States)
2017
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The detection method based on the mathematical expectation (ME) strategy is fast and accuracy for low frequency mutation screening in large samples. Previous studies have found that 14-bp insertion/deletion (indel) variants of 3′ untranslated region (3′ UTR) within bovine PRNP gene been characterized with (≤5%) global breeds outside China, which has not determined Chinese cattle yet. Therefore, this study aimed to identify indel 5 major indigenous evaluate its associations phenotypic traits....
Sperm-associated antigen 17 (SPAG17) gene encodes a multifunctional cytoplasmic protein, which influences not only reproduction but also skeletal development related body measurement traits, especially height. Thus, this study aimed to identify crucial insertion-deletion (indel) variations, influence the traits of goats in large goat populations (n = 1725). As result, two intronic indels (14 bp and indel) were identified by sequencing. For indel loci, distributions genotypes alleles...
The proliferation and differentiation of preadipocytes is an important factor determining bovine fat development, which closely related to the feed conversion ratio, carcass traits, beef quality. purpose this study was identify effects candidate circRNA miRNA on in order provide basic materials for molecular breeding cattle. sequencing performed adipocyte samples at different time points, a total 1830 differentially expressed circRNAs were identified. Among them, circBDP1, derived from BDP1...
Reactive oxygen species (ROS) are important factors that lead to a decline in sperm quality during semen preservation. Excessive ROS accumulation disrupts the balance of antioxidant system and causes lipid oxidative damage, destroying its structure function. Curcumin is natural plant extract neutralizes enhances function endogenous enzymes. The effect curcumin on preservation sheep has not been reported. This study aims determine effects refrigerated (4 °C) analyze metabolism from Chinese...
Abstract Adipocyte growth and development are complex precisely orchestrated processes. Several microRNAs have been identified as critical regulators of the adipocyte development. Recently, bta‐miR‐204 was found to be involved in adipogenesis; however, underlying molecular mechanism bta‐miR‐204‐mediated regulation proliferation, differentiation, apoptosis adipocytes is not fully understood or elucidated. In this study, quantitative real‐time polymerase chain reaction (qRT‐PCR), Cell Counting...
DNA methylation (DNAm), a major element of epigenetics, plays critical roles in individual development. Reduced representation bisulfite sequencing (RRBS) is an effective and economical method for analyzing the single base. The aims this study were to determine DNAm profiles contexts (CGs non-CGs) lactation dry periods goat mammary glands using RRBS, identify potential milk-related genes. proportion CG was highest among all sequence contexts. levels (72.44% 75.24%) occurred 3' UTR region,...
Adipogenesis describes the proliferation, differentiation, and apoptosis of mature adipocytes from primary is regulated by post-transcriptional modifications. Circular RNAs (circRNAs) play critical roles in mammalian development physiology. However, circRNA-mediated regulation adipogenesis remains poorly understood. We profiled circRNA expression during bovine adipogenesis, detecting 16 candidates, including circPPARγ, which was abundant adipose tissue. Overexpression (overexpression...
In practical production, almost all rams and about 50% of ewes are used to fatten. Researchers have proved that ewe ovariectomy could improve the productivity significantly, but specific molecular mechanism is still unknown. this study, five independent cDNA libraries (three two from ovariectomized normal longissimus dorsi samples, respectively) were constructed thoroughly explore global transcriptome, further reveal how influence muscle development by Illumina2000 sequencing technology. As...
Adipocyte differentiation-associated long noncoding RNA (ADNCR) is a newly discovered lncRNA. It plays function by targeting miR-204 to significantly regulates the expression of target SIRT1 gene in preadipocytes both at level mRNA and protein, thereby inhibiting adipogenesis. The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) strategy fast accuracy negligible cost for SNP genotyping large samples. In study, novel g.1263T>A intron 1 bovine ADNCR was found. Herein,...
Skeletal muscle is one of the three major types in an organism and has key roles motor system, metabolism, homeostasis. RNA-Seq analysis showed that novel lncRNA, lncFAM200B , was differentially expressed embryonic, neonatal, adult cattle skeletal muscles. The main aim this study to investigate molecular expression characteristics along with its crucial genetic variations. Our results bovine a 472 nucleotide (nt) non-coding RNA containing two exons. transcription factor binding site...
The SMAD family member 2 (SMAD2), a of the TGF-beta superfamily, executes significant part in oogenesis and ovulation process. A genome-wide selective sweep analysis also found SMAD2 was different fertility groups Laoshan dairy goats; whether this gene linked to litter size unknown. Therefore, chosen study its effects on Shaanbei white cashmere goat reproduction mRNA expression profile. Herein, level firstly determined female tissues, revealing differences levels tissues (p < 0.05),...
Abstract Adipogenesis is closely related to human health, livestock growth, and meat quality. A previous study identified that bovine lncFAM200B promoter has high activity in 3T3‐L1 mice preadipocytes. Thus, was a candidate gene for regulating adipogenesis. This aimed uncover the role of adipogenesis identify novel genetic variations within gene. An expression analysis found expressed higher fat than muscle, but difference not total methylation level active region. Moreover, exhibited...
As an important part of the circadian rhythm, regulation factor 2 cryptochrome (CRY2), regulates many physiological functions. Previous studies have reported that CRY2 is involved in growth and development. However, relationship between gene polymorphism cattle carcass traits remains unclear. The aim this study was to detect possible variations elucidate association Shandong Black Cattle Genetic Resource (SDBCGR) population (n = 705). We identified a 24-bp deletion variation (CRY2-P6) 6-bp...
Objective: This study aimed to analyze the molecular mechanism of heterosis in East Friesian sheep×Hu sheep (EH) hybrid and Suffolk×EH (SHE) (Ovis aries).Methods: In this research, growth performance data Hu (H), EH SHE from birth 8 months age were analyzed. Three 8-month-old each three strains (9 total) chosen their longissimus dorsi muscles collected for transcriptome sequencing. We verified expression seven differentially expressed genes (DEGs) by real-time quantitative reverse...
Paired-like homeodomain 2 (PITX2), a key gene in hypothalamic-pituitary-adrena axis, influences animal growth and development. The objective of this study was to identify the association functional genetic variations within goat PITX2 with traits mRNA expression levels. According reported single nucleotide polymorphisms (SNPs) information Guanzhong dairy (GZDG), we identified genotypes known SNPs Hainan black (HNBG). Association analysis uncovered that AC_000163: g.18117T > C, g.18161C G...