A5100737322- Genetic and phenotypic traits in livestock
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Hypothalamic control of reproductive hormones
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Ruminant Nutrition and Digestive Physiology
- RNA modifications and cancer
- Muscle Physiology and Disorders
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Gestational Trophoblastic Disease Studies
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related gene regulation
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Neuroendocrine regulation and behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- 3D Printing in Biomedical Research
- Plant and fungal interactions
- Renal and related cancers
- Garlic and Onion Studies
Anhui Agricultural University
2012-2024
Poultry Research Institute
2024
University of Louisville
1994-1995
Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding.Here, we describe induced pluripotent (iPS) derived bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins.Bovine iPS showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) expressed Nanog SSEA1.The CpG the promoter regions Oct4 were highly unmethylated compared to fibroblasts.The able...
Ovarian follicular development and hormone secretion are complex coordinated biological processes which will usually be altered during pregnancy. function is tightly regulated by a multitude of genes, also some specific miRNAs. It necessary to identify the differentially expressed miRNAs in ovaries pregnant non-pregnant mammals, order further understand role miRNA-mediated post-transcriptional regulation mammalian reproduction. Here, we performed comprehensive search for hircine using two...
Induced pluripotent stem cells (iPSCs) are somatic reprogrammed by ectopic expression of transcription factors or small molecule treatment, which resemble embryonic (ESCs). They hold great promise for improving the generation genetically modified large animals. However, few porcine iPSCs (piPSCs) lines obtained currently can support development cloned embryos. Here, we generated from adipose-derived (pADSCs), using drug-inducible defined human (Oct4, Sox2, c-Myc and Klf4). Reprogramming...
Long noncoding RNAs (lncRNAs) are involved in regulating animal development, however, their function the onset of puberty goats remain largely unexplored. To identify genes controlling regulation goats, we measured lncRNA and mRNA expression levels from hypothalamus. We applied RNA sequencing analysis to examine hypothalamus pubertal (case; n = 3) prepubertal (control; goats. Our results showed 2943 predicted lncRNAs, including 2012 differentially expressed which corresponded 5412 target...
Female pubertal development is tightly controlled by complex mechanisms, including neuroendocrine and epigenetic regulatory pathways. Specific gene expression patterns can be influenced DNA methylation changes in the hypothalamus, which turn regulate timing of puberty onset. In order to understand relationship between hypothalamus goats, whole-genome bisulfite sequencing RNA-sequencing analyses were carried out. There was a decline levels during 268 differentially methylated regions (DMR)...
Egg production is the most important trait of laying hens. To identify molecular markers and candidate genes associated with egg quality, such as body weight at first oviposition (BWF), number eggs produced in 500 days (EN500), (EW), shell thickness (EST), strength (ESS), Haugh unit (HU), a genome-wide analysis was performed 266 LingKun Chickens. The results showed that thirty-seven single nucleotide polymorphisms (SNPs) were all traits ( p < 9.47 × 10 −8 , Bonferroni correction)....
Age at puberty is an important factor affecting goat fertility, with endocrine and genetic factors playing a crucial role in the onset of puberty. To better understand relationship between mechanisms underlying goats, reproductive hormone levels were analyzed by ELISA ultraperformance liquid chromatography-multiple reaction monitoring-multistage/mass spectrometry RNA sequencing was performed to analyze ovarian genes. Serum follicle stimulating hormone, luteinizing estradiol,...
Abstract All‐ trans retinoic acid ( t ‐ RA ) is a natural component and representative physiologically active metabolite of vitamin A , having multiple physiologic functions. The objective this study was to evaluate the effect on goat oocyte maturation cumulus cell apoptosis during in vitro IVM ). Immature cumulus‐oocyte complexes COCs were matured absence or presence at concentrations 10 nmol/L, 100 nmol/L 1000 nmol/L. Oocyte embryo development assessed by polar body formation...
Ovary grafting is not only a method of investigating follicle and oocyte development, but also useful model to explore the possibility re-establishment reproductive axis in male-to-female sexual reversal. This study investigated ovary survival development after mouse ovaries were transplanted into immune-intact castrated male mice. Ten-day-old back muscle adult outbred mice treated with immunosuppressants. Twenty-two days later, structure number follicles present was examined by hematoxylin...
Long non-coding RNA (lncRNA) is involved in many biological processes, and it has been closely investigated. However, research into the role of lncRNA ovine ovarian development scant poorly understood, particularly relation to molecular mechanisms oocyte maturation. In current study, sequencing was performed with germinal vesicle (GV) vitro matured metaphase II (MII) stage oocytes, isolated from ewes. Through use bioinformatic analysis, abundant candidate lncRNAs stage-specific oocytes were...
This experiment was conducted to investigate the effects of dietary supplementation with different levels coated sodium butyrate (CSB) and polysaccharides extracted from Cordyceps cicadae (CCP) on growth performance, intestinal tissue morphology ileum microbiome in squabs. A total 420 1-day-old squabs were randomly divided into seven groups 5 replicates each 12 per replicate. The fed basal diet (control group) supplemented CSB (275, 550, 1,100 mg/kg, CSB-275, CSB-550, CSB-1100) CCP (27.5,...
RNA sequencing performed on goat matured oocytes and preimplantation embryos generated invivo enabled us to define the transcriptome for embryo development. The largest proportion of changes in gene expression was found at 16-cell stage, not as previously defined 8-cell is later than other mammalian species. In all, 6482 genes were identified be significantly differentially expressed across all consecutive developmental stage comparisons, important signalling pathways involved each...
Introduction This study evaluated the effects of Isatis Leaf (ISL) on growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum urine metabolites, epithelial transcriptome fattening sheep. Methods Twelve 3.5-month-old healthy sheep were randomly divided into two groups, each with 6 replicates, fed basal diet (CON) supplemented 80 g/kg ISL for 2.5 months. Gastrointestinal tract was collected histological analysis, fluid feces subjected to...
Abstract To investigate the effect of Y-27632 on low-temperature metabolism sheep sperm, different concentrations were added to semen at 4 °C in this experiment detect indicators such as sperm motility, plasma membrane, acrosome, antioxidant performance, mitochondrial membrane potential (MMP), and metabolomics. The results showed that addition 20 µM significantly increased integrity rate, acrosome capacity, MMP level, adenosine triphosphate (ATP) total cholesterol content, reduced Ca2+...
Objective: This study aimed to analyze the molecular mechanism of heterosis in East Friesian sheep×Hu sheep (EH) hybrid and Suffolk×EH (SHE) (Ovis aries).Methods: In this research, growth performance data Hu (H), EH SHE from birth 8 months age were analyzed. Three 8-month-old each three strains (9 total) chosen their longissimus dorsi muscles collected for transcriptome sequencing. We verified expression seven differentially expressed genes (DEGs) by real-time quantitative reverse...
Epidermal growth factor (EGF) stimulates the secretion of hCG in choriocarcinoma cells. However, molecular mechanisms involved this EGF action have never previously been investigated. The present study investigated them as well regulation hCG/LH (LH) receptor gene JEG-3 human cells contain multiple messenger RNA (mRNA) transcripts and a single 170-kilodalton immunoreactive protein. can bind to protein stimulate autophosphorylation phosphorylation four other membrane proteins. Culturing with...
This study explored the role of γ-aminobutyric acid transaminase (GABA-T) in puberty and reproductive performance female rats. Immunofluorescence technique, quantitative real-time PCR (RT-qPCR) enzyme-linked immunosorbent assay (ELISA) were used to detect distribution GABA-T expression genes hormones rats, respectively. The results showed that was mainly distributed arcuate nucleus (ARC), paraventricular (PVN) periventricular (PeN) hypothalamus, adenohypophysis, ovarian granulosa cells...