A5033813955- Genetic and phenotypic traits in livestock
- Hypothalamic control of reproductive hormones
- Cancer-related molecular mechanisms research
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Regulation of Appetite and Obesity
- Growth Hormone and Insulin-like Growth Factors
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Ovarian function and disorders
- Meat and Animal Product Quality
- Adipose Tissue and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hormonal and reproductive studies
- RNA modifications and cancer
- Neuropeptides and Animal Physiology
- Renal and related cancers
- Birth, Development, and Health
- Cancer-related gene regulation
- Plant Reproductive Biology
Anhui Agricultural University
2016-2025
Beijing Center for Disease Prevention and Control
2021
Institute for Animal Reproduction
2009
Pluripotent stem cells from domesticated animals have potential applications in transgenic breeding.Here, we describe induced pluripotent (iPS) derived bovine fetal fibroblasts by lentiviral transduction of Oct4, Sox2, Klf4 and c-Myc defined-factor fusion proteins.Bovine iPS showed typical colony morphology, normal karyotypes, stained positively for alkaline phosphatase (AP) expressed Nanog SSEA1.The CpG the promoter regions Oct4 were highly unmethylated compared to fibroblasts.The able...
Abstract Nesfatin-1 is an important molecule in the regulation of reproduction. However, its role reproductive axis male animals remains to be understood. Here, we found that nesfatin-1 was mainly distributed arcuate nucleus (ARC), paraventricular (PVN), periventricular (PeN), and lateral hypothalamic area (LHA) hypothalamus; adenohypophysis Leydig cells rats. Moreover, concentrations serum mRNA hypothalamo-pituitary-gonadal (HPGA) vary with age rat. After intracerebroventricular injection...
Previous studies have confirmed that methylation regulates gene transcription in the hypothalamus-pituitary-gonadal axis during puberty initiation, but little is known about regulation of DNA on expression pineal gland. To screen gland candidate genes related to onset goat and regulated by genome methylation, we collected glands from prepubertal pubertal female goats, then, determined profile whole bisulfite sequencing transcriptome RNA Illumina HiSeqTM2500. We analyzed differentially...
Abstract Immunological castration can be an alternative to traditional surgical castration. The active immunization against GnRH or kisspeptin has a castrating effect. To date, the fusion protein vaccine of combination with and have not been studied. Thus, present study will develop GnRH6-kisspeptin by genetic engineering method investigate its immunocastration effect in male rats. Twenty 20-day-old rats were randomly divided into two groups: control group ( n =10) =10). initial took place...
Long noncoding RNAs (lncRNAs) are involved in regulating animal development, however, their function the onset of puberty goats remain largely unexplored. To identify genes controlling regulation goats, we measured lncRNA and mRNA expression levels from hypothalamus. We applied RNA sequencing analysis to examine hypothalamus pubertal (case; n = 3) prepubertal (control; goats. Our results showed 2943 predicted lncRNAs, including 2012 differentially expressed which corresponded 5412 target...
The low full-term developmental efficiency of porcine somatic cell nuclear transfer (SCNT) embryos is mainly attributed to imperfect epigenetic reprogramming in the early embryos. However, dynamic expression patterns histone methylation involved progression during SCNT embryo development remain be unknown. In this study, we characterized and compared multiple markers including transcriptionally repressive (H3K9me2, H3K9me3, H3K27me2, H3K27me3, H4K20me2 H4K20me3) active modifications...
Female pubertal development is tightly controlled by complex mechanisms, including neuroendocrine and epigenetic regulatory pathways. Specific gene expression patterns can be influenced DNA methylation changes in the hypothalamus, which turn regulate timing of puberty onset. In order to understand relationship between hypothalamus goats, whole-genome bisulfite sequencing RNA-sequencing analyses were carried out. There was a decline levels during 268 differentially methylated regions (DMR)...
Abstract Background Circular RNA (circRNA) is produced during the splicing of mRNA (in addition to linear splicing) and part gene regulatory network. The temporal expression patterns different developmental stages were inseparable from these molecules’ function. Results Skeletal muscles Anhui white goat (AWG) across seven fetal postnatal development sequenced 21 sequencing libraries constructed. We thereby identified 9090 circRNAs analyzed their molecular properties, patterns, potential...
Age at puberty is an important factor affecting goat fertility, with endocrine and genetic factors playing a crucial role in the onset of puberty. To better understand relationship between mechanisms underlying goats, reproductive hormone levels were analyzed by ELISA ultraperformance liquid chromatography-multiple reaction monitoring-multistage/mass spectrometry RNA sequencing was performed to analyze ovarian genes. Serum follicle stimulating hormone, luteinizing estradiol,...
Abstract All‐ trans retinoic acid ( t ‐ RA ) is a natural component and representative physiologically active metabolite of vitamin A , having multiple physiologic functions. The objective this study was to evaluate the effect on goat oocyte maturation cumulus cell apoptosis during in vitro IVM ). Immature cumulus‐oocyte complexes COCs were matured absence or presence at concentrations 10 nmol/L, 100 nmol/L 1000 nmol/L. Oocyte embryo development assessed by polar body formation...
A series of complex processes regulate muscle development, and lncRNAs play essential roles in the regulation skeletal myogenesis. Using RNA sequencing, we profiled lncRNA expression during goat (Capra hircus) which included seven stages across fetal 45 (F45), 65 (F65), 90 (F90), 120 (F120), 135 (F135) days, born for 24 h (B1) (B90) days. total 15,079 were identified stages, they less conservative with other species (human, cow, mouse). Among them, 547 differentially expressed, divided into...
Long non-coding RNAs (lncRNAs) play important roles in almost all biological processes. However, there is little information on the effects of lncRNAs ovulation and lambing rates. In present study, we used high-throughput RNA sequencing to identify differentially expressed between ovaries multiparous (Mul) uniparous (Uni) Anhui White goats. Among 107,255,422 clean reads, 183,754 were significantly Uni Mul. them, 455 co-expressed two samples, whereas, 157,523 uniquely Uni, 25,776 Through Cis...
MicroRNA-1 (miR-1) has been shown to play an important role in muscle growth and development, however, it was mainly discovered model animals. To explore the function mechanism of miR-1 goat, we firstly explored expression profile goat tissues cells. Furthermore, target gene predicted, relationship between one its genes, histone deacetylase 4 (HDAC4), analyzed through double luciferase reporter assay, real-time PCR, western blot. It found that is most abundantly expressed heart skeletal...
Ovary grafting is not only a method of investigating follicle and oocyte development, but also useful model to explore the possibility re-establishment reproductive axis in male-to-female sexual reversal. This study investigated ovary survival development after mouse ovaries were transplanted into immune-intact castrated male mice. Ten-day-old back muscle adult outbred mice treated with immunosuppressants. Twenty-two days later, structure number follicles present was examined by hematoxylin...
Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability. However, little known about function lncRNAs (long noncoding RNAs) puberty. Therefore, RNA-seq analysis were performed between goats rats to clarify roles mRNAs In present study, length lncRNAs, open reading frame exon count compared two species. Furthermore, functional annotation based on Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) target genes differentially expressed...
MicroRNAs (miRNAs) are indispensable for the regulation of skeletal muscle. We performed RNA sequencing (RNA-seq) to establish a comprehensive miRNA profiling goat seven stages, including 45 (F45), 65 (F65), 90 (F90), 120 (F120), 135 (F135) days fetuses, newborn (B1) and born (B90) kids. Totally, 421 known miRNAs 228 novel were identified in data, average abundance 19 stages exceeds 10,000 reads per million (RPM). Furthermore, 420 differentially expressed (DEmiRNAs) all comparison group at...
The present study was designed to determine the effects of ghrelin on in vivo and vitro secretion testosterone (T) expression androgen receptor (AR) mRNA adult rat testis. distribution growth hormone secretagogue receptors (GHS-R1a) testis also investigated. GHS-R1a immunoreactivity presented mainly Sertoli Leydig cells, primary spermatocytes, secondary spermatocytes. Adult rats that were intracerebroventricularly (i.c.v.) administrated different dosages (1 nmol 3 nmol) could significantly...
There are many variables affecting the onset of puberty in animals, including genetic, nutritional, and environmental factors. Recent studies suggest that epigenetic regulation, especially DNA methylation, plays a majorrole regulation puberty. However, there have been no reports on methylation pubertal genome. We investigated female rat hypothalamus at prepuberty using reduced representation bisulfite sequencing technology. The identified genes signaling pathways exhibiting changes to rats...