A5100706229- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Hypothalamic control of reproductive hormones
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Tissue Engineering and Regenerative Medicine
- Regulation of Appetite and Obesity
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Biochemical effects in animals
- Growth Hormone and Insulin-like Growth Factors
- Medicinal Plant Pharmacodynamics Research
- Catalytic Processes in Materials Science
- Plant tissue culture and regeneration
- Chemical Synthesis and Analysis
- Virus-based gene therapy research
Hebei Medical University
2024
Zhongyuan University of Technology
2024
University of Benin
2024
Third Hospital of Hebei Medical University
2024
Anhui Agricultural University
2014-2023
Fuyang Normal University
2023
Longyan University
2023
Shenyang Pharmaceutical University
2022
People's Hospital of Kaizhou District
2021-2022
Shandong University
2022
Abstract Nesfatin-1 is an important molecule in the regulation of reproduction. However, its role reproductive axis male animals remains to be understood. Here, we found that nesfatin-1 was mainly distributed arcuate nucleus (ARC), paraventricular (PVN), periventricular (PeN), and lateral hypothalamic area (LHA) hypothalamus; adenohypophysis Leydig cells rats. Moreover, concentrations serum mRNA hypothalamo-pituitary-gonadal (HPGA) vary with age rat. After intracerebroventricular injection...
Ovarian follicular development and hormone secretion are complex coordinated biological processes which will usually be altered during pregnancy. function is tightly regulated by a multitude of genes, also some specific miRNAs. It necessary to identify the differentially expressed miRNAs in ovaries pregnant non-pregnant mammals, order further understand role miRNA-mediated post-transcriptional regulation mammalian reproduction. Here, we performed comprehensive search for hircine using two...
Superior kidding rate is an important economic trait in production of meat goat, and ovulation the precondition rate. MicroRNAs (miRNAs) play critical roles almost all ovarian biological processes, including folliculogenesis, follicle development, atresia, luteal development regression. To find out different activity recruitment with miRNA-mediated posttranscriptional regulation, small RNAs expressed pattern tissues multiple uniparous Anhui White goats during follicular phase was analyzed...
Diabetes mellitus is a well-recognized risk factor for periodontitis. The goal of the present study was to elucidate whether oxidative stress and nuclear erythroid 2-related 2 (Nrf2) participate in aggravation periodontitis by diabetes. For this purpose, we assigned Wistar rats control, periodontitis, diabetes, diabetic groups. Two weeks after induction diabetes streptozotocin, induced ligation. later, periodontal tissues blood were harvested analyzed stereomicroscopy, immunohistochemistry,...
Induced pluripotent stem cells (iPSCs) are somatic reprogrammed by ectopic expression of transcription factors or small molecule treatment, which resemble embryonic (ESCs). They hold great promise for improving the generation genetically modified large animals. However, few porcine iPSCs (piPSCs) lines obtained currently can support development cloned embryos. Here, we generated from adipose-derived (pADSCs), using drug-inducible defined human (Oct4, Sox2, c-Myc and Klf4). Reprogramming...
Long noncoding RNAs (lncRNAs) are involved in regulating animal development, however, their function the onset of puberty goats remain largely unexplored. To identify genes controlling regulation goats, we measured lncRNA and mRNA expression levels from hypothalamus. We applied RNA sequencing analysis to examine hypothalamus pubertal (case; n = 3) prepubertal (control; goats. Our results showed 2943 predicted lncRNAs, including 2012 differentially expressed which corresponded 5412 target...
Female pubertal development is tightly controlled by complex mechanisms, including neuroendocrine and epigenetic regulatory pathways. Specific gene expression patterns can be influenced DNA methylation changes in the hypothalamus, which turn regulate timing of puberty onset. In order to understand relationship between hypothalamus goats, whole-genome bisulfite sequencing RNA-sequencing analyses were carried out. There was a decline levels during 268 differentially methylated regions (DMR)...
Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating changes in mRNA expression levels cells and tissues. Selection proper reference genes very when calibrating results real-time PCR. Studies on selection goat tissues are limited, despite economic importance their meat dairy products. We used to detect eight gene candidates (18S, TBP, HMBS, YWHAZ, ACTB, HPRT1, GAPDH EEF1A2) ten types sourced from Boer goats. The optimal combination was selected according...
Abstract All‐ trans retinoic acid ( t ‐ RA ) is a natural component and representative physiologically active metabolite of vitamin A , having multiple physiologic functions. The objective this study was to evaluate the effect on goat oocyte maturation cumulus cell apoptosis during in vitro IVM ). Immature cumulus‐oocyte complexes COCs were matured absence or presence at concentrations 10 nmol/L, 100 nmol/L 1000 nmol/L. Oocyte embryo development assessed by polar body formation...
Abstract Melatonin (MLT) is an endogenous hormone with roles in animal germ cell development. However, the effect of MLT on porcine oocyte maturation and its underlying mechanisms remain largely unknown. Here, we investigated effects exogenous maturation, histone acetylation, autophagy subsequent embryonic We found that 1 nmol/L supplemented medium was optimal concentration to promote developmental competence quality parthenogenetic embryos. Interestingly, beneficial treatment embryo...
A series of complex processes regulate muscle development, and lncRNAs play essential roles in the regulation skeletal myogenesis. Using RNA sequencing, we profiled lncRNA expression during goat (Capra hircus) which included seven stages across fetal 45 (F45), 65 (F65), 90 (F90), 120 (F120), 135 (F135) days, born for 24 h (B1) (B90) days. total 15,079 were identified stages, they less conservative with other species (human, cow, mouse). Among them, 547 differentially expressed, divided into...
Long non-coding RNAs (lncRNAs) play important roles in almost all biological processes. However, there is little information on the effects of lncRNAs ovulation and lambing rates. In present study, we used high-throughput RNA sequencing to identify differentially expressed between ovaries multiparous (Mul) uniparous (Uni) Anhui White goats. Among 107,255,422 clean reads, 183,754 were significantly Uni Mul. them, 455 co-expressed two samples, whereas, 157,523 uniquely Uni, 25,776 Through Cis...
Ovary grafting is not only a method of investigating follicle and oocyte development, but also useful model to explore the possibility re-establishment reproductive axis in male-to-female sexual reversal. This study investigated ovary survival development after mouse ovaries were transplanted into immune-intact castrated male mice. Ten-day-old back muscle adult outbred mice treated with immunosuppressants. Twenty-two days later, structure number follicles present was examined by hematoxylin...
Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability. However, little known about function lncRNAs (long noncoding RNAs) puberty. Therefore, RNA-seq analysis were performed between goats rats to clarify roles mRNAs In present study, length lncRNAs, open reading frame exon count compared two species. Furthermore, functional annotation based on Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) target genes differentially expressed...
MicroRNAs (miRNAs) are indispensable for the regulation of skeletal muscle. We performed RNA sequencing (RNA-seq) to establish a comprehensive miRNA profiling goat seven stages, including 45 (F45), 65 (F65), 90 (F90), 120 (F120), 135 (F135) days fetuses, newborn (B1) and born (B90) kids. Totally, 421 known miRNAs 228 novel were identified in data, average abundance 19 stages exceeds 10,000 reads per million (RPM). Furthermore, 420 differentially expressed (DEmiRNAs) all comparison group at...
There are many variables affecting the onset of puberty in animals, including genetic, nutritional, and environmental factors. Recent studies suggest that epigenetic regulation, especially DNA methylation, plays a majorrole regulation puberty. However, there have been no reports on methylation pubertal genome. We investigated female rat hypothalamus at prepuberty using reduced representation bisulfite sequencing technology. The identified genes signaling pathways exhibiting changes to rats...
Summary The present study was to investigate if a completely chemically defined medium (PZM-4) could support the early development of porcine embryos derived from parthenogenetic activation (PA) and cloning (somatic cell nuclear transfer, SCNT), lay foundation for determining physiological roles certain supplements in this medium. Porcine PA SCNT were cultured media: PZM-3 (a semi-defined medium), PZM-4 fully PZM-5 (an undefined medium). Early embryo observed. We found that three groups...