A5100376189- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cellular transport and secretion
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Ubiquitin and proteasome pathways
- X-ray Spectroscopy and Fluorescence Analysis
- Microtubule and mitosis dynamics
- Topic Modeling
- Molecular Biology Techniques and Applications
- Protein Structure and Dynamics
- Magnetic Properties of Alloys
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Cloud Computing and Resource Management
- PARP inhibition in cancer therapy
- Advanced X-ray Imaging Techniques
- RNA Interference and Gene Delivery
Peking University
2015-2025
Harbin Institute of Technology
2016-2025
Jiangnan University
2019-2025
Wuxi Fourth People's Hospital
2025
Peking University First Hospital
2024-2025
Zhejiang University of Technology
2025
Hubei University
2024-2025
Imperial College London
2015-2024
Chongqing University
2024
Emory University
2024
The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. spectral features observed reflect molecular dominant absorbing atoms and provide contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was energies near carbon K edge identify map separate phases in various polymer blends DNA distribution...
Lianzhe Huang, Dehong Ma, Sujian Li, Xiaodong Zhang, Houfeng Wang. Proceedings of the 2019 Conference on Empirical Methods in Natural Language Processing and 9th International Joint (EMNLP-IJCNLP). 2019.
CHD7 is a member of the chromodomain helicase DNA binding domain family ATP-dependent chromatin remodeling enzymes. De novo mutation gene major cause CHARGE syndrome, genetic disease characterized by complex constellation birth defects ( C oloboma eye, H eart defects, A tresia choanae, severe R etardation growth and development, G enital abnormalities, E ar abnormalities). To gain insight into function CHD7, we mapped distribution protein on using approach immunoprecipitation tiled...
Protein tyrosine phosphatase (PTP) receptor T (PTPRT) is the most frequently mutated PTP in human cancers. However, cell signaling pathways regulated by PTPRT have not yet been elucidated. Here, we report identification of signal transducer and activator transcription 3 (STAT3) as a substrate PTPRT. Phosphorylation at amino acid Y705 essential for function STAT3, specifically dephosphorylated STAT3 this position. Accordingly, overexpression normal colorectal cancer cells reduced expression...
NSF and p97 are ATPases required for the heterotypic fusion of transport vesicles with their target membranes homotypic organelles. uses ATP hydrolysis to dissociate NSF/SNAPs/SNAREs complexes, separating v- t-SNAREs, which then primed subsequent rounds fusion. In contrast, does not p97/p47/SNARE complex even in presence ATP. Now we have identified a novel essential factor p97/p47-mediated membrane fusion, named VCIP135 (valosin-containing protein [VCP][p97]/p47 complex-interacting protein,...
Spt4/5 in archaea and eukaryote its bacterial homolog NusG is the only elongation factor conserved all three domains of life plays many key roles cotranscriptional regulation recruiting other factors to elongating RNA polymerase. Here, we present crystal structure as well polymerase-Spt4/5 complex using cryoelectron microscopy reconstruction single particle analysis. The binds middle polymerase claw encloses DNA, reminiscent DNA clamp ring helicases. transcription model reveals that an...
Abstract Background Pancreatoduodenectomy (PD) for pancreatic cancer is very challenging to many surgeons. Information regarding the advantage of using Da Vinci robotic system over conventional open surgery PD rare. Therefore, a comparison outcomes performed with was conducted. Methods Between January 2009 and December sixteen patients underwent PD, eight receiving robot‐assisted surgical (Group I) being treated II). Results There no significant difference in radical resection (R0) rate...
Replication Protein A (RPA), the major eukaryotic single stranded DNA-binding protein, binds to exposed ssDNA protect it from nucleases, participates in a myriad of nucleic acid transactions and coordinates recruitment other important players. RPA is heterotrimer coats long stretches single-stranded DNA (ssDNA). The precise molecular architecture subunits its binding domains (DBDs) during assembly poorly understood. Using cryo electron microscopy we obtained 3D reconstruction trimerisation...
p97/valosin-containing protein (VCP) is a type II ATPase associated with various cellular activities that forms homohexamer each protomer containing an N-terminal domain (N-domain); two domains, D1 and D2; disordered C-terminal region. Little known about the role of N-domain or region in p97 cycle. In p97-associated human disease inclusion body myopathy Paget bone frontotemporal dementia, majority missense mutations are located at interface. Structure-based predictions suggest such affect...
Answer selection plays a key role in community question answering (CQA). Previous research on answer usually ignores the problems of redundancy and noise prevalent CQA. In this paper, we propose to treat different text segments differently design novel attentive interactive neural network (AI-NN) focus those useful selection. The representations are first learned by convolutional networks (CNNs) or other architectures. Then AI-NN learns interactions each paired two texts. Row-wise...
Without discourse connectives, classifying implicit relations is a challenging task and bottleneck for building practical parser. Previous research usually makes use of one kind framework such as PDTB or RST to improve the classification performance on relations. Actually, under different annotation frameworks, there exist multiple corpora which have internal connections. To exploit combination corpora, we design related tasks specific corpus, propose novel Convolutional Neural Network...
Introduction Phage is promising for the treatment of hypervirulent Klebsiella pneumoniae (hvKP) infections. Although phage resistance seems inevitable, we found that there still was optimization space in therapy hvKP infection. Methods The clinical isolate K. FK1979 used to recover lysis ΦFK1979 from hospital sewage. Phage-resistant bacteria were obtained on LB agar and phages plaque assay, transmission electron microscopy (TEM), multiplicity infection test, one-step growth curve genome...