A5078738730- Retinoids in leukemia and cellular processes
- Antioxidant Activity and Oxidative Stress
- Effects of Radiation Exposure
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Immune Cell Function and Interaction
- RNA regulation and disease
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
- Circular RNAs in diseases
- Retinal Development and Disorders
- Cytomegalovirus and herpesvirus research
- Congenital heart defects research
- Glaucoma and retinal disorders
- Ophthalmology and Visual Impairment Studies
- Mitochondrial Function and Pathology
- HIV Research and Treatment
- Heat shock proteins research
- DNA Repair Mechanisms
- Wound Healing and Treatments
- Protein Tyrosine Phosphatases
- Reproductive System and Pregnancy
- Acute Myeloid Leukemia Research
University of Maryland, Baltimore
2015-2025
University of Maryland, College Park
2025
University of Baltimore
2016
Johns Hopkins University
2011
National Institute for Viral Disease Control and Prevention
2010
Case Comprehensive Cancer Center
2008
Case Western Reserve University
2007
Antibodies sustain viral control For many infected individuals, antiretroviral therapy (ART) means that an HIV-1 diagnosis is no longer a death sentence. But the virus persists in treated and complying with intense drug regimen to keep loads down can be challenging for patients. Seeking alternative, Byrareddy et al. ART-suppressed monkeys antibodies targeting α4β7 integrin. When ART was halted antibody-treated animals, stayed undetectable, normal CD4 T cell counts were maintained over 9...
Protein tyrosine phosphatase (PTP) receptor T (PTPRT) is the most frequently mutated PTP in human cancers. However, cell signaling pathways regulated by PTPRT have not yet been elucidated. Here, we report identification of signal transducer and activator transcription 3 (STAT3) as a substrate PTPRT. Phosphorylation at amino acid Y705 essential for function STAT3, specifically dephosphorylated STAT3 this position. Accordingly, overexpression normal colorectal cancer cells reduced expression...
Abstract Purpose Myopia incidence is increasing globally. All- trans retinoic acid (atRA) important in myopigenic retinoscleral signaling, motivating research on its ocular transport. However, atRA’s weak autofluorescence limits direct visualization tissues. Further, atRA hydrophobic and must bind to protein carriers for We assessed a fluorescent analog of (LightOx TM 14, CAS:198696-03-6, referred as ‘floRA’), an experimentally accessible surrogate by: (i) evaluating binding carrier proteins...
Abstract Background MicroRNAs are ~22-nt long regulatory RNAs that serve as critical modulators of post-transcriptional gene regulation. The diversity miRNAs in endothelial cells (ECs) and the relationship this to epithelial hematologic is unknown. We investigated baseline miRNA signature human ECs cultured from aorta (HAEC), coronary artery (HCEC), umbilical vein (HUVEC), pulmonary (HPAEC), microvasculature (HPMVEC), dermal (HDMVEC), brain (HBMVEC) understand expression ECs. Results...
How developmental programs reactivate in regeneration is a fundamental question biology. We addressed this through the study of Wound Induced Hair follicle Neogenesis (WIHN), an adult organogenesis model where stem cells regenerate de novo hair follicles following deep wounding. The exact mechanism uncertain. Here we show that self-noncoding dsRNA activates anti-viral receptor toll like 3 (TLR3) to induce intrinsic retinoic acid (RA) synthesis pattern predicts new formation after wounding...
Differentiation therapy with all-trans retinoic acid (atRA) has markedly improved outcome in acute promyelocytic leukemia (APL) but had little clinical impact other AML sub-types. Cell intrinsic mechanisms of resistance have been previously reported, yet the majority blasts are sensitive to atRA vitro. Even APL, single agent induces remission without cure. The microenvironment expression cytochrome P450 (CYP)26, a retinoid-metabolizing enzyme was shown determine normal hematopoietic stem...
Retinoic acid (RA), an essential active metabolite of vitamin A, controls numerous physiological processes. In addition to the analytical challenges owing its geometric isomers, low endogenous abundance, and often localized occurrence, nonspecific interferences observed during liquid chromatography (LC) multiple reaction monitoring (MRM) quantification methods have necessitated lengthy obtain accurate free interferences. We report development validation a fast high performance (HPLC)...
Perivascular stromal cells (PSCs) are a recently identified cell type that comprises small percentage of the platelet derived growth factor receptor-β+ within CNS perivascular space. PSCs activated following injury to brain or spinal cord, expand in number and contribute fibrotic scar formation site. Beyond fibrosis, their high density lesion core makes them potential significant source signals act on neural adjacent Our developmental analysis PSCs, defined by expression Collagen1a1 maturing...
In mammals, all-trans retinoic acid (ATRA) is instrumental to spermatogenesis. It synthesized by two retinaldehyde dehydrogenases (RALDH) present in both Sertoli cells (SC) and germ (GC). order determine the relative contributions of each source ATRA, we have generated mice lacking all RALDH activities seminiferous epithelium (SE). We show that SC- GC-derived sources ATRA cooperate initiate propagate spermatogenetic waves at puberty. adults, they exert redundant functions and, against...
Abstract Myopia, or near-sightedness, is rapidly growing in prevalence, with significant long-term implications for ocular health. There thus great impetus to better understand molecular signaling pathways leading myopia. We and others have reported that all-trans retinoic acid (atRA) involved myopigenic signaling, yet the understanding of how atRA transported exerts a influence poor. Here we measured concentrations serum wild-type C57BL/6 mice under control conditions after feeding,...
All-trans retinoic acid (atRA) plays a critical role in tissue homeostasis as master regulator of cellular proliferation, apoptosis and differentiation well immune cell function. An active metabolite vitamin A, atRA has been reported to be reduced number inflammatory conditions both the lung gut. Decreases have gastrointestinal bowel diseases, radiation-induced injury viral infection. In lung, is including allergic asthma injury; however, impact infection on not defined. this short...
Purpose: Ambient light exposure is linked to myopia development in children and affects susceptibility animal models. Currently, it unclear which signals mediate the effects of on myopia. All-trans retinoic acid (atRA) dopamine (DA) oppositely influence experimental may be involved retinoscleral signaling cascade underlying myopic eye growth. However, how ocular atRA responds different lighting whether DA interact remains unknown. Methods: Dark-adapted C57BL/6J mice (29–31 days old) were...
// Huabo Wang 1 , Jay Chauhan 2 Angela Hu Kelsey Pendleton Jeremy L. Yap Philip E. Sabato 3 Jace W. Jones Mariarita Perri 4 Jianshi Yu Erika Cione Maureen A. Kane 2,5 Steven Fletcher and Edward V. Prochownik 1,6,7 Section of Hematology/Oncology, Children's Hospital Pittsburgh UPMC Department Pharmaceutical Sciences, University Maryland School Pharmacy, Baltimore, MD, USA PharmD Program, Pharmaco-Biology, Calabria, Rende (CS), Italy 5 Marlene Stewart Greenebaum Cancer Center, 6 Microbiology...
Ocular all-trans retinoic acid (atRA) levels are influenced by visual cues, and exogenous atRA has been shown to increase eye size in chickens guinea pigs. However, it is not clear whether induces myopic axial elongation via scleral changes. Here, we test the hypothesis that will induce myopia alter biomechanics mouse.Male C57BL/6J mice were trained voluntarily ingest + vehicle (1% sugar, 25 mg/kg) (RA: n = 16 animals) or only (Ctrl: 14 animals). Refractive error (RE) ocular biometry...
Abstract Background Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with NP. Results Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) matrix-assisted laser desorption/ionization time flight (MALDI-TOF) mass spectrometry, we found only proteasome subunit p42...
Cellular retinol-binding protein, type I (CrbpI), encoded by 1 (Rbp1), is a chaperone of vitamin A (retinol) that epigenetically silenced in ~25% human breast cancers. CrbpI delivers to enzymes for metabolism into an active metabolite, all-trans retinoic acid (atRA), where atRA essential cell proliferation, apoptosis, differentiation, and migration. Here, we show the effect loss on mammary homeostasis using Rbp1–/– mouse model. tissue has disrupted retinoid results 40% depleted endogenous...
All-trans-retinoic acid (RA), a vitamin A metabolite, is an important signaling molecule required for the proper development of heart. The epicardium main source RA in embryonic heart, yet car-diogenic functions epicardial-produced are not fully understood. Here, we investigated roles using vivo and vitro models excess or deficiency RA. Our results suggested that facilitates cytoskeletal rearrangement epicardial-to-mesenchymal transition epicardial cells. In treatment with inhibitor...
Although low circulating levels of the vitamin A metabolite, all-trans retinoic acid (ATRA), are associated with increased risk cardiovascular events and all-cause mortality, few studies have addressed whether cardiac retinoid altered in failing heart. Here, we showed that proteomic analyses human guinea pig heart failure (HF) were consistent a decline resident ATRA. Quantitation retinoids ventricular myocardium by mass spectrometry revealed 32% 39% ATRA decreases HF patients idiopathic...
The β-carotene oxygenase 1 (BCO1) is the enzyme responsible for cleavage of to retinal, first intermediate in vitamin A formation. Preclinical studies suggest that BCO1 expression required dietary affect lipid metabolism. goal this study was generate a gene therapy strategy over-expresses adipose tissue and utilizes stored adipocytes produce reduce obesity.We generated novel adipose-tissue-specific, adeno-associated vector over-express (AT-AAV-BCO1) murine adipocytes. We tested using unique...
Purpose: The sclera is believed to biomechanically influence eye size, facilitating the excessive axial elongation that occurs during myopigenesis. Here, we test hypothesis will be remodeled and exhibit altered biomechanics in mouse model of form-deprivation (FD) myopia, accompanied by retinoid concentrations, a potential signaling molecule involved process. Methods: Male C57 Bl/6J mice were subjected unilateral FD (n = 44 eyes), leaving contralateral untreated (contra; n 44). Refractive...
Abstract Receptor protein tyrosine phosphatase T (PTPRT/PTPρ) is frequently mutated in human cancers including colon, lung, gastric, and skin cancers. More than half of the identified tumor-derived mutations are located extracellular part PTPρ. However, functional significance those domain remains to be defined. Here we report that PTPρ mediates homophilic cell-cell aggregation. This interaction very specific because does not interact with its closest homologue, PTPμ, a cell aggregation...
Lipids represent biologically ubiquitous and highly dynamic molecules in terms of abundance structural diversity. Whereas the potential for lipids to inform on disease/injury is promising, their unique characteristics make detection identification from biological samples analytically demanding. We report use ultraperformance convergence chromatography (UPC2 ), a variant supercritical fluid chromatography, coupled high-resolution, data-independent tandem mass spectrometry characterization...
Abstract Radiation exposure to the gastrointestinal system contributes acute radiation syndrome in a dose- and time-dependent manner. Molecular mechanisms that lead remain incompletely understood. Using murine model of total-body irradiation, C57BL/6J male mice were irradiated at 8, 10, 12, 14 Gy assayed day 1, 3, 6 after compared nonirradiated (sham) controls. Tryptic digests tissues (upper ileum) analyzed by liquid chromatography-tandem mass spectrometry on Waters nanoLC coupled Thermo...