A5100376198- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- FOXO transcription factor regulation
- MicroRNA in disease regulation
- Mechanisms of cancer metastasis
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Urinary Bladder and Prostate Research
- Wnt/β-catenin signaling in development and cancer
- Renal Transplantation Outcomes and Treatments
- ATP Synthase and ATPases Research
- Genital Health and Disease
- Prostate Cancer Diagnosis and Treatment
- Adenosine and Purinergic Signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Genomics and Diagnostics
- Heat shock proteins research
- Autophagy in Disease and Therapy
- Acute Kidney Injury Research
- Complement system in diseases
- Ferroptosis and cancer prognosis
- Galectins and Cancer Biology
Third Affiliated Hospital of Southern Medical University
2024
Shandong University
2024
Capital Medical University
2008-2023
Beijing Friendship Hospital
2022-2023
Beijing Chao-Yang Hospital
2022
Wenzhou Medical University
2022
First Affiliated Hospital of Wenzhou Medical University
2022
Qiqihar Medical University
2022
Wuhan University
2013-2015
University of Alabama at Birmingham
2013
Following its tyrosine phosphorylation, STAT3 is methylated on K140 by the histone methyl transferase SET9 and demethylated LSD1 when it bound to a subset of promoters that activates. Methylation negative regulatory event, because blockade greatly increases steady-state amount activated expression many (i.e., SOCS3 ) but not all CD14 target genes. Biological relevance shown observation overexpression cannot be blocks ability cells activate in response IL-6. methylation does occur with...
Molecular dynamics and hybrid quantum mechanics/molecular mechanics have been used to investigate the mechanisms of (+)AdoMet methylation protein-Lys-NH(2) catalyzed by lysine methyltransferase enzymes: histone monomethyltransferase SET7/9, Rubisco large-subunit dimethyltransferase, viral trimethyltransferase, Tyr245Phe mutation SET7/9. At neutrality in aqueous solution, primary amines are protonated. The enzyme reacts with Lys-NH(3)(+) species provide an Enz.Lys-NH(3)(+).(+)AdoMet complex....
We collected paired samples of tumor and adjacent normal colorectal tissues from 22 patients with carcinoma to compare the differences in expression lysine specific demethylase 1 (LSD1) these two tissues. The results showed that 19 (86.4%), LSD1 is more highly expressed tissue than tissue. To explore role tumorigenesis, we used somatic cell gene targeting generate an knockout (KO) HCT 116 human cancer line as a research model. analysis phenotypic changes KO cells are less tumorigenic, both...
Epigenetic reprogramming reportedly has a crucial role in prostate cancer (PCa) progression. RNA modification is hot topic epigenetics, and N6-methyladenosine (m6A) accounts for approximately 60% of chemical modifications. The aim this study was to evaluate the m6A patterns PCa patients construct risk prediction model using regulators.Analyses were based on levels 25 regulators Cancer Genome Atlas (TCGA). Differentially expressed gene (DEG) survival analyses performed according TCGA-PRAD...
Carbapenem-resistant Escherichia coli (CREC) is one of the most significant clinical pathogens, primarily emerging owing to widespread use broad-spectrum antibiotics. Antimicrobial resistance a major global health challenge that prolongs treatment duration and increases healthcare costs. This study evaluated antibacterial anti-inflammatory effects antimicrobial peptide Lf-KR against CREC. Broth microdilution method, growth curve analysis, time-kill assays were performed evaluate activity The...
Transcription regulation emerged to be one of the key mechanisms in regulating autophagy. Inhibitors H3K9 methylation activates expression LC3B, as well other autophagy-related genes, and promotes autophagy process. However, detailed regulated by nuclear factors remain elusive. In this study, we performed a drug screen SMYD2-/- cells discovered that SMYD2 deficiency enhanced cell death induced BIX01294, an inhibitor histone methylation. BIX-01294 induces accumulation LC3 II death, but not...
Mei Wang, Congjing Yu, Mianwen Li, Xinru Zhang, Kexin Jiang, Zhiyong Xiaodong Zhang
Abstract Receptor protein tyrosine phosphatase T (PTPRT/PTPρ) is frequently mutated in human cancers including colon, lung, gastric, and skin cancers. More than half of the identified tumor-derived mutations are located extracellular part PTPρ. However, functional significance those domain remains to be defined. Here we report that PTPρ mediates homophilic cell-cell aggregation. This interaction very specific because does not interact with its closest homologue, PTPμ, a cell aggregation...
Molecular dynamics (MD) simulations and hybrid quantum mechanics/molecular mechanics (QM/MM) calculations have been carried out in an investigation of histone lysine methyltransferase (SET7/9). Proton dissociation (SET7/9·Lys4-NH3+·AdoMet → SET7/9·Lys4-NH2·AdoMet + H+) must be prior to the methylation by S-adenosylmethionine (AdoMet). We find that a water channel is formed allow escape proton solvent. The appears presence AdoMet, but not present species SET7/9·Lys4-NH3+ or...
The catalysis by SET7/9 histone lysine methyltransferase of AdoMet N-methylation the transcriptional factor p53-Lys4-NH2 has been investigated with particular attention paid to means product specificity. After formation SET7/9·p53-Lys4-NH3+·AdoMet complex, following events occur: (i) appearance a water channel, (ii) depronation p53-Lys4-NH3+ via this channel into aqueous solvent, and (iii) methylation form p53-Lys4-N(Me)H2+. does not occur on SET7/9·p53-Lys4-NH3+,...
Molecular dynamics simulations employing a molecular mechanics (MM) force field and hybrid quantum (QM) MM (QM/MM) have been carried out to investigate the product specificity mechanism of histone H4 lysine 20 (H4-K20) methylation by human methyltransferase SET8. At neutral pH, target is available only enzyme in protonated state. The first step reaction must be deprotonation which followed (+)AdoMet [Enz.Lys-CH(2)-NH(3)(+).(+)AdoMet --> H(+) + Enz.Lys-CH(2)-NH(2).(+)AdoMet -->-->...
We collected paired samples of tumor and adjacent normal colorectal tissues from 22 patients with carcinoma to compare the differences in expression lysine specific demethylase 1 (LSD1) these two tissues.The results showed that 19 (86.4%),LSD1 is more highly expressed tissue than tissue.To explore role LSD1 tumorigenesis, we used somatic cell gene targeting generate an knockout (KO) HCT 116 human cancer line as a research model.The analysis phenotypic changes KO cells are less tumorigenic,...
There are three reaction steps in the S-adenosylmethionine (AdoMet) methylation of lysine-NH2 catalyzed by a methyltransferase. They (i) combination enzyme·Lys-NH3+ with AdoMet, (ii) substrate ionization to provide enzyme·AdoMet·Lys-NH2, and (iii) methyl transfer providing enzyme·AdoHcy·Lys-N(Me)H2+ dissociation AdoHcy. In this study viral histone methyltransferase (vSET), we find that vSET·Lys27-NH3+, vSET·Lys27-N(Me)H2+, vSET·Lys27-N(Me)2H+ takes place upon AdoMet. The presence water...
Background Filamentous fungal infections are associated with a high morbidity and mortality in solid organ transplants. The present study aimed to investigate the aspergillus pneumonia renal transplant recipients, its diagnosis as well treatment. Methods Approximately 2000 cases of transplants were retrospectively studied we focused on hospitalized during August 1, 2005 February 2007, period. clinical database electronic records analyzed. Recently published literature was reviewed. Results...
N-cadherin (cadherin-2 [CDH2]) is widely known as the promoter of prostate cancer (PCa) invasion and castration resistance. However, biological mechanism in PCa progression unclear. In this study, we overexpressed LNCaP cells downregulated PC3 by lentiviral transduction. Then, differentially expressed genes (DEGs) dysregulated functions were investigated through RNA sequencing (RNA-seq) analyses. We found 13 long noncoding (lncRNA) transcripts, 72 messenger (mRNA) 3 integrated N-cadherin....
Abstract Objectives Bladder cancer (BCa) is the most common malignant tumor in urinary system. Growing evidence suggests that as a suppressor gene, hypermethylated 1 (HIC1) correlated with various malignancies modulation of progression. This study aims to investigate effect HIC1 on regulating proliferation, migration, and invasion BCa. Methods Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) Western blot (WB) were used evaluate expression messenger RNA protein human BCa...
Objective: To evaluate the efficacy and safety of Hengli ® Chinese botulinum toxin type A (BTX-A; 100 U) in patients with overactive bladder. Methods: This study was a multicenter, randomized, double-blind, placebo-controlled trial who were inadequately managed anticholinergic medications. Eligible randomized 2:1 to receive intradetrusor injections BTX-A ( n = 144) or placebo 72). The primary endpoint change number daily micturition episodes at week 6 from baseline. secondary endpoints...
Background Epigenetic reprogramming through dysregulated histone lysine methylation (HLM) plays a crucial role in prostate cancer (PCa) progression. This study aimed to comprehensively evaluate HLM modification patterns PCa microenvironment infiltration. Materials and methods Ninety-one regulators The Cancer Genome Atlas (TCGA) dataset were analyzed using bioinformatics. Differentially expressed genes (DEGs) survival analyses performed TCGA-PRAD clinicopathologic follow-up information....
Bloom syndrome protein (BLM) is known to maintain genomic integrity including DNA repair, recombination, replication and transcription. Its dysregulation affects the instability of cells, which results in a high risk developing various types cancer even syndrome. However, date, best our knowledge, no association has been made between human BLM bladder cancer. Thus, aim present study was investigate role The expression pattern tissue detected by immunohistochemistry. viability, proliferation,...
Abstract Introduction Dapoxetine on demand has been approved for premature ejaculation (PE) management in China; however, studies the efficacy and safety of this treatment Chinese population are scarce. Aim The aim study was to evaluate effectiveness dapoxetine 30 mg 60 men with PE. Methods Phase IV real-world 1,252 patients If reported no response after 4 weeks treatment, uptitrated at more. Main Outcome Measure Self-reported data were collected demographics, general sexual health...
Na, K-ATPase interaction (NKAIN) is a transmembrane protein family, which can interact with β1 subunit. NKAIN1 plays an important role in alcohol-dependent diseases such as endometrial and prostate cancers. However, the relationship between human breast cancer has not been studied. Hence, this study aimed to explore expression cancer. Data used were mainly from Cancer Genome Atlas, including differential analysis, Kaplan-Meier survival receiver operating characteristic curve multiple Cox...