A5002570522- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Neonatal and fetal brain pathology
- Anesthesia and Neurotoxicity Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiac Arrest and Resuscitation
- Ion Channels and Receptors
- Neurological Disease Mechanisms and Treatments
- Acute Ischemic Stroke Management
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Ischemia and Reperfusion
- Mitochondrial Function and Pathology
- Immune cells in cancer
- Nicotinic Acetylcholine Receptors Study
- S100 Proteins and Annexins
- Biochemical effects in animals
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Estrogen and related hormone effects
- Intensive Care Unit Cognitive Disorders
- Aortic Disease and Treatment Approaches
- Medicinal Plants and Neuroprotection
- Blood properties and coagulation
The Ohio State University
2022-2025
Neurological Surgery
2021-2025
The Ohio State University Wexner Medical Center
2024
University of Colorado Anschutz Medical Campus
2014-2023
University of Colorado Denver
2013-2022
San Antonio Uniformed Services Health Education Consortium
2020
Joint Base San Antonio
2020
Brooke Army Medical Center
2020
Uniformed Services University of the Health Sciences
2020
Tri-County Health Department
2020
Evaluation of infarct volumes and infiltrating immune cell populations in mice after middle cerebral artery occlusion (MCAO) strongly implicates a mixture both pathogenic regulatory subsets stroke pathogenesis recovery. Our goal was to evaluate the contribution B cells development MCAO by comparing functional outcomes wild-type (WT) versus B-cell-deficient μMT<sup>−/−</sup> mice. The results clearly demonstrate larger volumes, higher mortality, more severe deficits, increased numbers...
Abstract Propulsion at the microscale requires unique strategies such as undulating or rotating filaments that microorganisms have evolved to swim. These features however can be difficult artificially replicate and control, limiting ability actuate direct engineered microdevices targeted locations within practical timeframes. An alternative propulsion strategy swimming is rolling. Here we report low-strength magnetic fields reversibly assemble wheel-shaped devices in situ from individual...
Emerging evidence suggests that renal endothelial function may be altered in ischemia-reperfusion injury. Acute kidney injury is sexually dimorphic, and estrogen protects tubular after experimental ischemic This study tested the hypothesis during ischemia-reperfusion, alters glomerular to prevent hyperpermeability. Glomerular cells were exposed 8-h oxygen-glucose deprivation (OGD) followed by 4- reoxygenation-glucose repletion. After 4-h repletion, transendothelial permeability Ficoll-70 was...
The calcium-permeable transient receptor potential M2 (TRPM2) ion channel is activated following oxidative stress and has been implicated in ischemic damage; however, little experimental evidence exists linking TRPM2 activation to damage cerebral ischemia. We directly assessed the involvement of channels brain injury using pharmacological inhibitors short-hairpin RNA (shRNA)-mediated knockdown expression. Each four tested provided significant protection male neurons vitro ischemia...
Septic patients frequently develop cognitive impairment that persists beyond hospital discharge. The impact of sepsis on electrophysiological and molecular determinants learning is underexplored. We observed mice survived or endotoxemia experienced loss hippocampal long-term potentiation (LTP), a brain-derived neurotrophic factor-mediated (BDNF-mediated) process responsible for spatial memory formation. Memory occurred despite preserved BDNF content could be reversed by stimulation...
Stroke-induced cerebral ischemia is a major cause of death and disability. The disruption blood flow results in neuronal glial cell leading to brain injury. Reperfusion restores oxygen the affected tissue, but can also damage through an enhanced oxidative stress inflammatory response. This study examines mitochondrial transfer from MSC neurons role it plays preservation after oxidant We observed mitochondria mouse vitro following hydrogen peroxide exposure. was dependent on cell-to-cell...
Males exhibit greater histologic and behavioral impairment after stroke than do age-matched females. However, the contribution of androgens to outcome remains unclear. We compared outcomes from middle cerebral artery occlusion (MCAO) in castrated mice with those testosterone- or dihydrotestosterone (DHT)-replaced mice. Castrates treated 1.5 mg testosterone 0.5 DHT before MCAO showed smaller infarct volumes (hemisphere: 27 26%) at 24 h 90 mins did untreated castrates (37%), whereas 5...
Background— Paraplegia continues to complicate thoracoabdominal aortic interventions. The elusive mechanism of spinal cord ischemia–reperfusion injury has delayed the development pharmacological adjuncts. Microglia, resident macrophages central nervous system, can have pathological responses after a variety insults. This occur through toll-like receptor 4 (TLR-4) in stroke models. We hypothesize that occlusion results from TLR-4–mediated microglial activation mice. Methods and Results— TLR-4...
Translation from basic science bench research in ischemic stroke to bedside treatment of patients suffering remains a difficult challenge. Despite literally hundreds compounds and interventions that provide benefit experimental models cerebral ischemia, efficacy humans be demonstrated. The reasons for failure translate the extensive positive findings successful clinical trials have been focus discussion years. Some attribute flaws trial design, others question predictive value current animal...
Thrombi that occlude blood vessels can be resolved with fibrinolytic agents degrade fibrin, the polymer forms between and around platelets to provide mechanical stability. Fibrinolysis rates however are often constrained by transport‐limited delivery penetration of fibrinolytics into thrombus. Here, these limitations overcome colloidal microwheel (µwheel) assemblies functionalized tissue‐type plasminogen activator (tPA) assemble, rotate, translate, eventually disassemble via applied magnetic...
The calcium-permeable transient receptor potential M2 (TRPM2) ion channel was recently demonstrated to have a sexually dimorphic contribution ischemic brain injury, with inhibition or knockdown of the protecting male preferentially. We tested hypothesis that androgen signaling is required for this male-specific cell-death pathway. Additionally, we differential activation enzyme poly (ADP-ribose) polymerase-1 (PARP-1) responsible TRPM2 and neuronal injury. observed administration inhibitor...
Perivascular stromal cells (PSCs) are a recently identified cell type that comprises small percentage of the platelet derived growth factor receptor-β+ within CNS perivascular space. PSCs activated following injury to brain or spinal cord, expand in number and contribute fibrotic scar formation site. Beyond fibrosis, their high density lesion core makes them potential significant source signals act on neural adjacent Our developmental analysis PSCs, defined by expression Collagen1a1 maturing...
DAPK1 binding to GluN2B was prominently reported mediate ischemic cell death in vivo. and CaMKII bind the same region, their is mutually exclusive. Here, we show that mutating region on (L1298A/R1300Q) protected against neuronal induced by cardiac arrest followed resuscitation. Importantly, mutation selectively abolished only CaMKII, but not DAPK1, binding. During or excitotoxic insults, further accumulated at excitatory synapses, this accumulation mediated Interestingly, extra-synaptic...
Fura-2 fluorescence was used to investigate the effects of H2O2 on [Ca2+]i in insulin-secreting cell line CRI-G1. (1–10 mm) caused a biphasic increase free [Ca2+]i, an initial rise observed within 3 min and second, much larger following 30-min exposure. Extracellular calcium removal blocked late, but not initial, [Ca2+]i. Thapsigargin did affect either response H2O2, activated capacitive entry, action abolished by 10 μm La3+. Simultaneous recordings membrane potential demonstrated same...
Whole‐cell current‐clamp recordings demonstrate that leptin (0.3–10 n m ) hyperpolarizes CRI‐G1 insulin‐secreting cells. This effect is slow on onset and not reversed washout of the leptin. Voltage‐clamp indicate activates a potassium conductance in presence intracellular ATP (5 mm), but has no its absence. Following activation ATP‐sensitive K + (K current by diazoxide (0.2 ), addition did alter cell membrane potential or further. The leptin‐induced hyperpolarization increased are completely...
In mouse hippocampal CA1 pyramidal neurons, the activity of synaptic small-conductance Ca(2+)-activated K(+) channels type 2 (SK2 channels) provides a negative feedback on N-methyl-D-aspartate receptors (NMDARs), reestablishing Mg(2+) block that reduces Ca(2+) influx. The well-established role NMDARs in ischemia-induced excitotoxicity led us to test neuroprotective effect modulating SK2 channel following cerebral ischemia induced by cardiac arrest and cardiopulmonary resuscitation (CA/CPR)....