A5080660695- Photoacoustic and Ultrasonic Imaging
- Cytokine Signaling Pathways and Interactions
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Thermoregulation and physiological responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Response and Inflammation
- Immune cells in cancer
- Hearing, Cochlea, Tinnitus, Genetics
- Neurogenesis and neuroplasticity mechanisms
- RNA Research and Splicing
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
- Glaucoma and retinal disorders
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- Diabetic Foot Ulcer Assessment and Management
- Phonocardiography and Auscultation Techniques
- Wound Healing and Treatments
- Receptor Mechanisms and Signaling
- Nerve injury and regeneration
- Nuclear Receptors and Signaling
- Noise Effects and Management
- Cancer, Hypoxia, and Metabolism
- Facial Rejuvenation and Surgery Techniques
Oregon Health & Science University
1999-2020
University of Washington
2011-2015
Ophthalmology Associates (United States)
2015
Northeastern University
2014
Portland VA Medical Center
2011
Institute of Occupational Medicine
2010
Massachusetts General Hospital
2004
Beth Israel Deaconess Hospital
1995
Evaluation of infarct volumes and infiltrating immune cell populations in mice after middle cerebral artery occlusion (MCAO) strongly implicates a mixture both pathogenic regulatory subsets stroke pathogenesis recovery. Our goal was to evaluate the contribution B cells development MCAO by comparing functional outcomes wild-type (WT) versus B-cell-deficient μMT<sup>−/−</sup> mice. The results clearly demonstrate larger volumes, higher mortality, more severe deficits, increased numbers...
The blood vessel morphology is known to correlate with several diseases, such as cancer, and important for describing tissue physiological processes, like angiogenesis. Therefore, a quantitative method characterizing the angiography obtained from medical images would have clinical applications. Optical microangiography (OMAG) obtaining three-dimensional of vessels within volume tissue. In this study we propose quantify OMAG spectral domain optical coherence tomography system. A technique...
Hypoxia is a prominent feature of malignant tumors that are characterized by angiogenesis and vascular hyperpermeability. Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) has been shown to be up-regulated in the vicinity necrotic tumor areas, hypoxia potently induces VPF/VEGF expression several cell lines vitro. Here we report hypoxia-induced mediated increased transcription mRNA stability human M21 melanoma cells. RNA-binding/electrophoretic mobility shift assays...
Reduced risk and severity of stroke in adult females is thought to depend on normal endogenous levels estrogen, a well-known neuroprotectant immunomodulator. In male mice, experimental induces immunosuppression the peripheral immune system, characterized by reduction spleen size cell numbers decreased cytokine chemokine expression. However, stroke-induced has not been evaluated female mice. To test hypothesis that estradiol (E2) deficiency exacerbates after focal females, we effect middle...
Estradiol is protective in experimental cerebral ischemia, but the precise mechanisms remain unknown. Signal transducer and activator of transcription-3 (STAT3) a transcription factor that activated by estrogen, translocates to nucleus, induces neuroprotective genes, such as bcl-2 . We determined whether estradiol increases STAT3 activation female rat brain after focal ischemia contributes estradiol-mediated neuroprotection against ischemic injury. Ovariectomized (OVX) rats with without...
Purpose: Soft tissue ischemia is a devastating and unpredictable complication following dermal filler injection. Multiple mechanisms to explain this have been proposed, including vascular compression, vessel damage, intraarterial emboli. To elucidate the mechanism of injury, authors introduce mouse model, imaged with optical microangiography laser speckle contrast imaging technologies, demonstrate in vivo microvascular response soft intravascular Methods: determine effect external...
A multi-functional imaging system capable of determining relative changes in blood flow, hemoglobin concentration, and morphological features the vasculature is demonstrated. The combines two non-invasive techniques, a dual-wavelength laser speckle contrast (2-LSI) an optical microangiography (OMAG) system. 2-LSI used to monitor dynamic flow concentration oxygenated (HbO), deoxygenated (Hb) total (HbT). OMAG acquire high resolution images functional vessel network. area density (VAD)...
Cutaneous wound healing consists of multiple overlapping phases starting with blood coagulation following incision vessels. We utilized label-free optical coherence tomography and microangiography (OMAG) to noninvasively monitor process dynamics microcirculation system in a mouse ear pinna model. Mouse is composed two layers skin separated by layer cartilage because its total thickness around 500 μm, it can be as an ideal model for imaging techniques. These are identical human structure...
We present a novel application of optical microangiography (OMAG) imaging technique for visualization depth-resolved vascular network within retina and choroid as well measurement total retinal blood flow in mice.A fast speed spectral domain OCT system at 820nm with line scan rate 140 kHz was developed to image the posterior segment eyes mice.By applying an OMAG algorithm extract moving signals out background tissue, we are able provide true capillary level choroidal vasculature.The...
In this Letter, we describe a newly developed synchronized dual-wavelength laser speckle contrast imaging system, which contains two cameras that are synchronously triggered to acquire data. The system can data at high spatiotemporal resolution (up 500 Hz for ∼1000×1000 pixels). A mouse model of stroke is used demonstrate the capability fast changes (within tens milliseconds) in oxygenated and deoxygenated hemoglobin concentration, relative blood flow brain, through an intact cranium. This...
The ability to non-invasively monitor and quantify hemodynamic responses down the capillary level is important for improved diagnosis, treatment management of neurovascular disorders, including stroke. We developed an integrated multi-functional imaging system, in which synchronized dual wavelength laser speckle contrast (DWLS) was used as a guiding tool optical microangiography (OMAG) test whether detailed vascular experimental stroke male mice can be evaluated with wide range sensitivity...
Optical microangiography (OMAG) and Doppler optical (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, blood flow velocity direction. These were used to visualize acute chronic microvascular responses upon an injury in vivo. A wound was induced using a 0.5 mm biopsy punch on mouse pinna. The changes microangiography, direction quantified for (<30 min) response structure determined (30 min–60 days). initial...
The "developmental origins of adult disease" hypothesis was originally derived from evidence linking low birth weight to cardiovascular diseases including stroke. Subsequently, it has been expanded include developmental exposures environmental contaminants as risk factors for onset disease.Our goal in this study test the that exposure poly-chlorinated biphenyls (PCBs) alters stroke outcome adults.We exposed rats PCB mixture Aroclor 1254 (A1254) at 0.1 or 1 mg/kg/day maternal diet throughout...
Oxygen availability is regarded as a critical factor to metabolically regulate systemic blood flow. There debate how peripheral flow (PBF) affected and modulated during hypoxia hyperoxia; however in vivo evaluating of functional PBF under oxygen-related physiological perturbation remains challenging. Microscopic observation, the current frequently used imaging modality for characterization often involves use exogenous contrast agents, which would inevitably perturb intrinsic physiologic...
A synchronized dual-wavelength laser speckle contrast imaging (DWLSCI) system and a Doppler optical microangiography (DOMAG) was developed to determine several ischemic parameters in the cochlea due systemic hypoxic challenge. DWLSCI can obtain two-dimensional data, used relative changes cochlear blood flow, change concentrations of oxyhemoglobin (HbO), deoxyhemoglobin (Hb) total hemoglobin (HbT) mice. DOMAG three-dimensional flow with single vessel resolution. It demonstrated that during...
Cholinergic differentiation factors (CDFs) suppress noradrenergic properties and induce cholinergic in sympathetic neurons. The CDFs leukemia inhibitory factor (LIF) ciliary neurotrophic (CNTF) bind to a LIFR·gp130 receptor complex activate Jak/signal transducers activators of transcription Ras/mitogen-activated protein kinases signaling pathways. Little is known about how these properties. We used neurons SK-N-BE(2)M17 neuroblastoma cells investigate CDF down-regulation the norepinephrine...
Reduced cochlear blood flow (CoBF) is a main contributor to hearing loss. Studying CoBF has remained challenge due the lack of available tools. Doppler optical microangiography (DOMAG), method quantify single-vessel absolute flow, and laser flowmetry (LDF), for measuring relative within large volume tissue, were used determining changes in systemic hypoxia mice. DOMAG determined change apical turn (AT) with resolution, while LDF averaged cochlea (hemisphere ∼1 1.5 mm radius). Hypoxia was...