A5103224508- Alzheimer's disease research and treatments
- Glaucoma and retinal disorders
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- Biochemical effects in animals
- Retinal Diseases and Treatments
- Metabolomics and Mass Spectrometry Studies
- 14-3-3 protein interactions
- Endoplasmic Reticulum Stress and Disease
- Cannabis and Cannabinoid Research
- Advanced Proteomics Techniques and Applications
- Pharmacological Effects of Natural Compounds
- Mass Spectrometry Techniques and Applications
- Advanced Glycation End Products research
- Redox biology and oxidative stress
- Cholinesterase and Neurodegenerative Diseases
- Neurotransmitter Receptor Influence on Behavior
- Pharmacogenetics and Drug Metabolism
- Gout, Hyperuricemia, Uric Acid
- Sperm and Testicular Function
- Neuroscience of respiration and sleep
- Genomics, phytochemicals, and oxidative stress
- Aldose Reductase and Taurine
- Autophagy in Disease and Therapy
- Neuroinflammation and Neurodegeneration Mechanisms
Chengdu University of Traditional Chinese Medicine
2014-2025
Guangdong Academy of Forestry
2018-2023
University of Louisville
2012-2022
Weatherford College
2020
Xian Mental Health Center
2019
Zhejiang Provincial Hospital of TCM
2019
Hangzhou Hospital of Traditional Chinese Medicine
2019
Xi'an Jiaotong University
2011-2014
Institute of Pharmacology
2008-2012
Hunan University of Science and Technology
2012
purpose. Based on the evidence of an amplified production reactive oxygen species (ROS) during glaucomatous neurodegeneration, proteomic analysis was performed to determine oxidative modification retinal proteins after experimental elevation intraocular pressure (IOP). methods. IOP induced in rats by hypertonic saline injections into episcleral veins. Protein expression determined two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) protein lysates obtained from eyes matched for...
This study aimed to determine retinal proteomic alterations in human glaucoma, with particular focus on links TNF-␣/TNFR1 signaling.METHODS.Human protein samples were obtained from 20 donors (n ϭ 10) or without glaucoma.Alterations expression individually analyzed by quantitative LC-MS/MS.Quantitative Western blot analysis cleavage phosphorylation site-specific antibodies was used for data validation, and cellular localization of selected proteins determined immunohistochemical the retina an...
Abstract Alzheimer's disease (AD) is a neurodegenerative disorder characterized by neurofibrillary tangles, senile plaques, and loss of synapses. Many studies support the notion that oxidative stress plays an important role in AD pathogenesis. Previous from our laboratory employed redox proteomics to identify oxidatively modified proteins inferior parietal lobule (IPL) hippocampus. The were consistent with biochemical or pathological alterations have been central further investigations...
The toxicity of the ubiquitous pollutant and endogenous metabolite, acrolein, is due in part to covalent protein modifications. Acrolein reacts readily with nucleophiles via Michael addition Schiff base formation. Potential acrolein targets include nucleophilic side chains cysteine, histidine, lysine residues as well free amino terminus proteins. Although cysteine most acrolein-reactive residue, cysteine−acrolein adducts are difficult identify vitro vivo. In this study, model peptides...
Aldehydes are ubiquitous pollutants generated during the combustion of organic materials and present in air, water, food. Several aldehydes also endogenous products lipid peroxidation by-products drug metabolism. Despite well-documented high reactivity unsaturated aldehydes, little is known regarding their cardiovascular effects role cardiac pathology. Accordingly, we examined myocardial model aldehyde acrolein. In closed-chest mice, intravenous acrolein (0.5 mg/kg) induced rapid but...
Objective— Atherosclerotic lesions are associated with the accumulation of reactive aldehydes derived from oxidized lipids. Although inhibition aldehyde metabolism has been shown to exacerbate atherosclerosis and enhance aldehyde-modified proteins in atherosclerotic plaques, no therapeutic interventions have devised prevent lesions. Approach Results— We examined efficacy carnosine, a naturally occurring β-alanyl-histidine dipeptide, preventing toxicity atherogenesis apolipoprotein E–null...
To delineate astrocyte-mediated inflammatory processes in glaucoma, we analyzed proteomic responses of retinal astrocytes an experimental rat model using a cell-specific approach.IOP elevation was induced rats by hypertonic saline injections into episcleral veins. Enriched samples were isolated through the immunomagnetic cell selection process established originally for ganglion (RGC) sampling. Ocular hypertensive and control collected pooling from eyes matched cumulative IOP exposure....
Alzheimer's disease (AD) is a central nervous system disorder pathologically characterized by senile plaques, neurofibrillary tangles, and synapse loss. A small percentage of individuals with normal antemortem psychometric scores, after adjustments f
Soluble urokinase plasminogen activator receptor (suPAR) is an immune-derived circulating signaling molecule that has been implicated in chronic kidney disease, such as focal segmental glomerulosclerosis (FSGS). Typically, native uPAR (isoform 1) translates to a 3-domain protein capable of binding and activating integrins, yet the function additional isoforms generated by alternative splicing unknown. Here, we characterized mouse isoform 2 (msuPAR2), encoding domain I nearly one-half II,...
Bruguiera gymnorrhiza (BG), a medicinal mangrove, and its fruit (a food material), have traditionally been used to treat diarrhea (also known as ulcerative colitis) in folk medicine. However, the mechanism of action against colitis remains ambiguous. This study aimed investigate potential efficacy BGF on experimental colitis. Colitis was induced by oral intake dextran sulfate sodium (DSS) treated with aqueous extract (25, 50 100 mg/kg) for week. The disease activity index (DAI), colon length...
Fluoridation causes an obvious reduction of dental caries by interference with cariogenic streptococci. However, the effect fluoride on group A streptococci that rheumatic fever and acute poststreptococcal glomerulonephritis is not known. We have used proteomic analysis to create a reference proteome map for<i>Streptococcus pyogenes</i> determine fluoride-induced protein changes in Cellular extracellular proteins were resolved two-dimensional polyacrylamide gel electrophoresis identified...