A5031388957- Acute Kidney Injury Research
- Cardiac, Anesthesia and Surgical Outcomes
- Religion, Society, and Development
- Biblical Studies and Interpretation
- Muscle and Compartmental Disorders
- Cardiac Arrest and Resuscitation
- Electrolyte and hormonal disorders
- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- Chronic Kidney Disease and Diabetes
- Intensive Care Unit Cognitive Disorders
- Mechanical Circulatory Support Devices
- Hemodynamic Monitoring and Therapy
- Anesthesia and Sedative Agents
- Anesthesia and Neurotoxicity Research
- Cardiac Ischemia and Reperfusion
- Christian Theology and Mission
- Heart Failure Treatment and Management
- Transplantation: Methods and Outcomes
- Pharmacological Effects and Toxicity Studies
- Hormonal Regulation and Hypertension
- Dialysis and Renal Disease Management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Renal Transplantation Outcomes and Treatments
- Electroconvulsive Therapy Studies
Oregon Health & Science University
2016-2025
Portland VA Medical Center
2018-2025
VA Portland Health Care System
2018-2021
University of Portland
2017-2021
University of Toronto
2020
University of California, San Francisco
2020
Veterans Health Administration
2018
Marymount University
2010
Bridge University
2008
Brigham and Women's Hospital
2005
Emerging evidence suggests that renal endothelial function may be altered in ischemia-reperfusion injury. Acute kidney injury is sexually dimorphic, and estrogen protects tubular after experimental ischemic This study tested the hypothesis during ischemia-reperfusion, alters glomerular to prevent hyperpermeability. Glomerular cells were exposed 8-h oxygen-glucose deprivation (OGD) followed by 4- reoxygenation-glucose repletion. After 4-h repletion, transendothelial permeability Ficoll-70 was...
Iron chelators have been widely used to remove excess toxic iron from patients with secondary overload. However, small molecule-based can cause adverse side effects such as infection, gastrointestinal bleeding, kidney failure, and liver fibrosis. Here we report renal clearable nanochelators for overload disorders. First, after a singledose intravenous injection, the nanochelator shows favorable pharmacokinetic properties, kidney-specific biodistribution rapid excretion (>80% injected dose in...
Significance Statement Rhabdomyolysis causes severe AKI and death in settings such as earthquakes armed conflict. Specific treatment is not available care difficult to provide these austere environments. Skeletal muscle myoglobin a renal toxin that this syndrome. Proximal tubular megalin participates endocytosis may be an mediator. The authors demonstrate mouse model proximal plays critical role rhabdomyolysis-induced AKI. In model, tubule–specific deletion ameliorated AKI, effect was...
Abstract Introduction There is a sex difference in the risk of ischemic acute kidney injury (AKI), and estrogen mediates protective effect female sex. We previously demonstrated that preprocedural chronic restoration physiologic to ovariectomized mice ameliorated AKI after cardiac arrest cardiopulmonary resuscitation (CA/CPR). In present study, we hypothesized male aged would benefit from administration CA/CPR. tested clinically relevant manner by administrating it Methods CA/CPR was...
Abstract Kidney intercalated cells are involved in acid-base homeostasis via vacuolar ATPase expression. Here we report six human cell subtypes, including hybrid principal-intercalated identified from single transcriptomics. Phagosome maturation is a biological process that increases pathway analysis rank following exposure to uropathogenic Escherichia coli two of the subtypes. Real time confocal microscopy visualization murine renal tubules perfused with green fluorescent protein expressing...
Acute kidney injury (AKI) increases the risk of in-hospital death, adds to expense care, and early chronic disease. AKI often follows an acute event such that timely treatment could ameliorate potentially reduce additional Despite therapeutic success dexamethasone in animal models, clinical trials have not demonstrated broad success. To improve safety efficacy for AKI, we developed characterized a novel, kidney-specific nanoparticle enabling specific within-kidney targeting proximal tubular...
Acute cardiorenal syndrome (CRS) represents a critical intersection of cardiac and renal dysfunction with profound clinical implications. Despite its significance, the molecular underpinnings that mediate cellular responses within kidney during CRS remain inadequately understood. We employed single nucleus RNA sequencing (snRNAseq) to dissect transcriptomic landscape following in translational model CRS, arrest/cardiopulmonary resuscitation (CA/PCR) comparison ischemia reperfusion injury...
Severe ischemia induces renal injury less frequently in women than men. In this study, cardiac arrest and cardiopulmonary resuscitation were used to assess whether estradiol is renoprotective via an estrogen receptor (ER)-dependent mechanism.Male female C57BL/6 ER gene-deleted mice underwent 10 min of followed by resuscitation. Serum chemistries stereology measured 24 h after arrest.Estrogen did not affect mean arterial pressure, regional cortical blood flow, gases. Hence, kidneys protected...
Acute cardiorenal syndrome is a common complication of acute cardiovascular disease. Studies kidney injury (AKI) to chronic disease (CKD) transition, including patients suffering disease, report high rates CKD development. Therefore, associates with CKD, but no study has established causation. To define this we used murine cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) model or sham procedure on male mice. CA was induced potassium chloride while CPR consisted chest compressions...
To report 3 cases of accidental dexmedetomidine overdose in the perioperative setting and review pathophysiology alpha2-agonist overdose. case summaries: Three patients accidentally received overdoses dexmedetomidine, one intraoperatively (192 microg over 20 min) 2 postoperatively (4 rather than 0.4 0.2 microg/kg/h; 0.5 microg/kg/min microg/kg/h). Hemodynamic parameters remained stable for all patients. The most notable sign was oversedation diagnosed either clinically or using a bispectral...
Serious complications are common during the intensive care of postoperative cardiac surgery patients. Some these may be influenced by communication process handover from operating room to unit (ICU) team. A structured transfer reduce rate errors and perioperative complications.We hypothesized that a collaborative, comprehensive, intraoperative team ICU would specific set complications. We tested this hypothesis developing introducing comprehensive multidisciplinary process. measured patient...
Cardiorenal syndrome type 1 causes acute kidney injury but is poorly understood; animal models and diagnostic aids are lacking. Robust noninvasive measurements of glomerular filtration rate required for clinical use. Several have been described untested in translational suffer from biologic interference. We developed a mouse model cardiorenal tested the novel near-infrared fluorophore ZW800-1 to assess renal cardiac function. performed murine arrest cardiopulmonary resuscitation followed by...
Introduction Acute kidney injury is a serious,sexually dimorphic perioperative complication, primarily attributed to hypoperfusion. We previously found that estradiol renoprotective after cardiac arrest and cardiopulmonary resuscitation in ovariectomized female mice. Additionally, we neither estrogen receptor alpha nor beta mediated this effect. hypothesized the G protein (GPR30) mediates effect of estrogen. Methods Ovariectomized gonadally intact male wild-type GPR30 gene-deleted mice were...
Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has high mortality1-4 and most frequently caused by whole-body hypoperfusion.5,6 Successful reproduction hypoperfusion in rodent models been fraught with difficulty.7-9,9,10 Models employ focal ischemia have repeatedly demonstrated results do not translate to the clinical setting, larger animal allow for whole body lack access full toolset genetic manipulation possible mouse.11,12 However, recent...
In preclinical investigations, ischemic preconditioning (IPC) protects kidneys from ischemia/reperfusion injury. The direct effects of IPC on glomerular endothelial cells have not been studied in detail. Most investigations focused healthy and animals, it remains unknown whether is renoprotective the setting medical comorbidities such as diabetes. this study, we determined preventive potential cell monolayers, compared these results to monolayers cultured under hyperglycemic conditions. We...