A5032027401- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Extracellular vesicles in disease
- Lipid Membrane Structure and Behavior
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Nanoplatforms for cancer theranostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Advanced Polymer Synthesis and Characterization
- Advanced Drug Delivery Systems
- Nanocluster Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Barrier Structure and Function Studies
- Electrochemical Analysis and Applications
- Virus-based gene therapy research
- Neurogenesis and neuroplasticity mechanisms
- Adipose Tissue and Metabolism
- Connexins and lens biology
- Biochemical effects in animals
- Pain Mechanisms and Treatments
- Free Radicals and Antioxidants
- Photoacoustic and Ultrasonic Imaging
- Drug Transport and Resistance Mechanisms
Duquesne University
2017-2024
University of North Carolina at Chapel Hill
2013-2017
Institute of Nanotechnology
2015-2016
CeNTech
2015-2016
Carolina Institute for NanoMedicine
2015
University of Nebraska Medical Center
2010-2013
Wayne State University
2004-2012
Nebraska Medical Center
2012
University of Nebraska–Lincoln
2010
We report the synthesis of temperature- and redox-responsive multiblock copolymers by reversible addition−fragmentation chain transfer (RAFT) polymerization. Well-defined α,ω-bis(dithioester)-functionalized poly(N-isopropylacrylamide) (PNIPAM) poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) were prepared using 1,4-bis(thiobenzoylthiomethyl)benzene 1,4-bis(2-(thiobenzoylthio)prop-2-yl)benzene as RAFT agents, respectively. Dually responsive synthesized in a single aminolysis/oxidation step...
Aims: Active targeted transport of the nanoformulated redox enzyme, catalase, in macrophages attenuates oxidative stress and as such increases survival dopaminergic neurons animal models Parkinson's disease. Optimization drug formulation is crucial for successful delivery living cells. We demonstrated earlier that packaging catalase into a polyion complex micelle ('nanozyme') with synthetic polyelectrolyte block copolymer protected enzyme against degradation improved therapeutic outcomes....
Brain-derived neurotrophic factor (BDNF) is identified as a potent neuroprotective and neuroregenerative agent for many neurological diseases. Regrettably, its delivery to the brain hampered by poor serum stability rapid clearance. Here, novel nanoformulation reported composed of bio-compatible polymer, poly(ethylene glycol)-b-poly(L-glutamic acid) (PEG-PLE), that hosts BDNF molecule in nanoscale complex, termed here Nano-BDNF. Upon simple mixture, Nano-BDNF spontaneously forms uniform...
End-functionalized poly(N-isopropylacrylamide) (PNIPA) was synthesized by living free radical polymerization and conventional used to prepare graft copolymers with poly(ethylenimine) (PEI). The exhibited lower critical solution temperature (LCST) behavior between 30 32 °C formed complexes plasmid DNA. LCST of the in DNA increased slightly ∼34−35 °C. Cytotoxicity evaluated measuring lactate dehydrogenase (LDH) release from cells. temperature-dependent toxicity, higher levels LDH observed at...
Polyplexes sensitive to redox potential gradients represent promising gene delivery vectors. High molecular weight polypeptides containing disulfide bonds in the backbone were synthesized by an oxidative copolymerization of a histidine-rich peptide (HRP) and nuclear localization sequence (NLS) peptide. The synthetic approach allowed easy synthesis reducible copolypeptides (rCPP) with different relative contents HRP NLS sequences. Cytotoxicity transfection activity rCPP-based DNA polyplexes...